AB238305

Anti-Phosphothreonine antibody [RM102]

Name: Anti-Phosphothreonine antibody [RM102] (ab238305) | Abcam
Brand: Abcam Limited
SKU: AB238305
Price: 575 USD
Availability: InStock

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(2 Publications)

Rabbit Monoclonal Phosphothreonine antibody. Suitable for IP, ELISA, WB, ICC/IF and reacts with Modified Amino Acid samples. Cited in 2 publications.

4 Images

Immunocytochemistry/ Immunofluorescence - Anti-Phosphothreonine antibody [RM102] (AB238305)

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-Phosphothreonine antibody [RM102] (AB238305)

Serum-starved A431 (human epidermoid carcinoma cell line) cells treated with Calyculin A/Okadaic Acid stained for Phosphothreonine using ab238305 at a 1/500 dilution in ICC/IF, followed by a PE conjugated secondary antibody (red). Counterstained with DAPI (blue).

Left panel : Non-treated cells.

Right panel : Cells treated with Calyculin A/Okadaic Acid.

Supplier Data

Immunoprecipitation - Anti-Phosphothreonine antibody [RM102] (AB238305)

Immunoprecipitation of Calyculin A/Okadaic Acid treated A431 (Human epidermoid carcinoma cell line) cells by ab238305 at a 1/500 dilution, and then blotted with ab238305.

Lane 1 : Whole lysate control.
Lane 2 : IP with rabbit IgG control.
Lane 3 : IP with ab238305.

All lanes:

Immunoprecipitation - Anti-Phosphothreonine antibody [RM102] (ab238305)

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ELISA - Anti-Phosphothreonine antibody [RM102] (AB238305)

ELISA

Supplier Data

ELISA - Anti-Phosphothreonine antibody [RM102] (AB238305)

ab238305 recognizes Phosphorylated threonine in peptides with different sequences.

It has minimal cross-reactivity with phosphorylated serine.

Supplier Data

Western blot - Anti-Phosphothreonine antibody [RM102] (AB238305)

All lanes:

Western blot - Anti-Phosphothreonine antibody [RM102] (ab238305) at 1/2000 dilution

Lane 1:

Serum starved A431 (human epidermoid carcinoma cell line) cell lysate

Lane 2:

Serum starved A431 cells treated with Calyculin A/Okadaic Acid lysate

false

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

RM102

Isotype

IgG

Carrier free

Applications

ICC/IF, WB, IP, ELISA

applications

Specificity

ab238305 reacts threonine-phosphorylated proteins. No cross reactivity with nonphosphorylated threonine, phosphoserine, and phosphotyrosine. It shows slight cross-reactivity with a few phospho-serine-containing peptides.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "ELISA" : {"fullname" : "ELISA", "shortname":"ELISA"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Modified Amino Acid": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "1/100 - 1/500", "IP-species-notes": "", "ELISA-species-checked": "testedAndGuaranteed", "ELISA-species-dilution-info": "", "ELISA-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/2000", "WB-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100 - 1/500", "ICCIF-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Purification notes

Purified from an animal origin–free culture supernatant.

Storage buffer

Preservative: 0.09% Sodium azide Constituents: PBS, 50% Glycerol (glycerin, glycerine), 1% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Phosphothreonine often called phospho-threonine refers to a modified amino acid where a phosphate group attaches to threonine. It usually forms during protein post-translational modifications. This modification commonly occurs in eukaryotic cells and plays a significant role in regulating protein function. The mass of phosphothreonine itself is approximately 181 Da. Phosphothreonine residues are found in various proteins where they serve as important components for controlling multiple cellular processes.

Biological function summary

Phosphorylation of threonine residues influences protein activity localization and interactions. These phosphorylated threonine residues often exist as part of a larger protein complex influencing the dynamics of protein interactions. In cellular signal transduction they act as key regulatory switches. The modification is a reversible process which allows dynamic control over protein functionality in response to cellular signals.

Pathways

Signaling cascades often rely on phosphorylation events involving threonine which includes pathways like MAPK and PI3K/AKT. These pathways are essential for cellular responses to external stimuli and involve several other proteins such as RAS and AKT themselves. Through such pathways the phosphorylation state of threonine residues affects decisions about cell survival proliferation or differentiation.

Dysregulation of threonine phosphorylation links to conditions like cancer and diabetes. Abnormal phosphorylation patterns can lead to unregulated cell growth and survival common in tumorigenesis. Proteins such as p53 and insulin receptor substrates interact with phosphorylated threonine residues in these contexts. Investigating these interactions provides insights into the pathological mechanisms and potential therapeutic targets.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

See full target information Phosphothreonine

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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Experimental hematology & oncology 14:47 PubMed40156013

2025

A one-base therapeutic insertion in the HBG2 distal promoter reactivates γ-globin expression.

Applications

Unspecified application

Species

Unspecified reactive species

Xiuqin Bao,Yuanyi Gao,Xiaoyi Chen,Zhongju Wang,Xiaoqin Feng,Liren Wang,Jing Du,Yuhua Ye,Feijing Chen,Li Du,Aihua Yin,Xiangmin Xu

FASEB journal : official publication of the Federation of American Societies for Experimental Biology 35:e21827 PubMed34383980

2021

Atypic SUMOylation of Nor1/NR4A3 regulates neural cell viability and redox sensitivity.

Applications

Unspecified application

Species

Unspecified reactive species

Jonathan Gagnon,Véronique Caron,Laurent Gyenizse,André Tremblay

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Phosphothreonine antibody [RM102]

Immunocytochemistry/ Immunofluorescence - Anti-Phosphothreonine antibody [RM102] (AB238305)

Immunoprecipitation - Anti-Phosphothreonine antibody [RM102] (AB238305)

ELISA - Anti-Phosphothreonine antibody [RM102] (AB238305)

Western blot - Anti-Phosphothreonine antibody [RM102] (AB238305)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Applications

Specificity

Reactivity data

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (2)

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