Rabbit Recombinant Monoclonal Phosphotyrosine antibody. Carrier free. Suitable for IP, ELISA, Dot, WB, ICC/IF, Flow Cyt (Intra) and reacts with Modified Amino Acid, Synthetic peptide samples.
Images
![Immunoprecipitation - Anti-Phosphotyrosine antibody [EPR16871] - BSA and Azide free (AB240219), expandable thumbnail](https://content.abcam.com/products/images/phosphotyrosine-antibody-epr16871-bsa-and-azide-free-ab240219--immunoprecipitation-img46428.jpg/_jcr_content/renditions/webp-475.webp "Immunoprecipitation - Anti-Phosphotyrosine antibody [EPR16871] - BSA and Azide free (AB240219)")
![Immunocytochemistry/ Immunofluorescence - Anti-Phosphotyrosine antibody [EPR16871] - BSA and Azide free (AB240219), expandable thumbnail](https://content.abcam.com/products/images/phosphotyrosine-antibody-epr16871-bsa-and-azide-free-ab240219--immunocytochemistry-immunofluorescence-img88372.jpg/_jcr_content/renditions/webp-475.webp "Immunocytochemistry/ Immunofluorescence - Anti-Phosphotyrosine antibody [EPR16871] - BSA and Azide free (AB240219)")
![ELISA - Anti-Phosphotyrosine antibody [EPR16871] - BSA and Azide free (AB240219), expandable thumbnail](https://content.abcam.com/products/images/phosphotyrosine-antibody-epr16871-bsa-and-azide-free-ab240219--elisa-img63318.jpg/_jcr_content/renditions/webp-475.webp "ELISA - Anti-Phosphotyrosine antibody [EPR16871] - BSA and Azide free (AB240219)")
![Dot Blot - Anti-Phosphotyrosine antibody [EPR16871] - BSA and Azide free (AB240219), expandable thumbnail](https://content.abcam.com/products/images/phosphotyrosine-antibody-epr16871-bsa-and-azide-free-ab240219--dot-blot-img17988.jpg/_jcr_content/renditions/webp-475.webp "Dot Blot - Anti-Phosphotyrosine antibody [EPR16871] - BSA and Azide free (AB240219)")
![Flow Cytometry (Intracellular) - Anti-Phosphotyrosine antibody [EPR16871] - BSA and Azide free (AB240219), expandable thumbnail](https://content.abcam.com/products/images/phosphotyrosine-antibody-epr16871-bsa-and-azide-free-ab240219--flow-cytometry-intracellular-img108181.jpg/_jcr_content/renditions/webp-475.webp "Flow Cytometry (Intracellular) - Anti-Phosphotyrosine antibody [EPR16871] - BSA and Azide free (AB240219)")
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| IP | ELISA | Dot | WB | ICC/IF | Flow Cyt (Intra) | |
|---|---|---|---|---|---|---|
| Modified Amino Acid | Tested | Tested | Not recommended | Expected | Tested | Tested |
| Synthetic peptide | Not recommended | Not recommended | Tested | Not recommended | Not recommended | Not recommended |
IP
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Modified Amino Acid | Dilution info - | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Synthetic peptide | Dilution info - | Notes - |
ELISA
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Modified Amino Acid | Dilution info - | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Synthetic peptide | Dilution info - | Notes - |
Dot
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Synthetic peptide | Dilution info - | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Modified Amino Acid | Dilution info - | Notes - |
WB
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Modified Amino Acid | Dilution info Use at an assay dependent concentration. | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Synthetic peptide | Dilution info - | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Modified Amino Acid | Dilution info - | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Synthetic peptide | Dilution info - | Notes - |
Flow Cyt (Intra)
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Modified Amino Acid | Dilution info - | Notes ab199376 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Synthetic peptide | Dilution info - | Notes - |
Associated Products
Select an associated product type
9 products for Alternative Product
3 products for Alternative Version
Target data
Alternative names
L-Phosphotyrosine, L-Tyrosine-O-phosphate, O-Phospho-L-tyrosine, O-Phosphotyrosine, Phospho-L-tyrosine, Phosphonotyrosine
Recommended products
Rabbit Recombinant Monoclonal Phosphotyrosine antibody. Carrier free. Suitable for IP, ELISA, Dot, WB, ICC/IF, Flow Cyt (Intra) and reacts with Modified Amino Acid, Synthetic peptide samples.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Carrier free
- Yes
- Clone number
- EPR16871
- Purification technique
- Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
- Storage information
- Do Not Freeze
Notes
ab240219 is the carrier-free version of Anti-Phosphotyrosine antibody [EPR16871] ab179530.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Phosphotyrosine also known simply as pY is a post-translational modification where a phosphate group binds to the hydroxyl group of a tyrosine amino acid in proteins. This small modification can have a huge impact on proteins' function and interaction. Phosphotyrosine has a molecular weight of approximately 216 Da when it stands on its own. This modification can occur in various proteins expressed widely across tissues playing a role in signaling cell division and morphological changes.
- Biological function summary
Phosphorylated tyrosine residues modify the function and activity of proteins within cells. It is a critical component of signal transduction pathways and can alter protein functions when becoming part of large protein complexes. It often regulates receptor proteins and intracellular kinases modifying their ability to interact with other proteins like SH2 and PTB domain-containing proteins. Phosphotyrosine antibodies like anti-phosphotyrosine Ig2 are helpful research tools for detecting these modifications.
- Pathways
The modification of tyrosine to phosphotyrosine participates significantly in pathways such as the MAPK/ERK pathway and the PI3K/AKT pathway. These pathways are important for various cellular processes including growth and survival. Proteins like EGFR and PDGFR regulate signaling cascades by phosphorylation of tyrosine residues and often interact with phosphotyrosine and phosphotyrosine antibodies importantly.
- Associated diseases and disorders
The dysregulation of phosphotyrosine levels contributes to conditions like cancer and diabetes. Aberrant activation or overexpression of phosphotyrosine residues on receptors like HER2 and insulin receptors can promote uncontrolled cell proliferation often seen in tumors and contribute to insulin resistance respectively. As such phosphotyrosine serves not only as a biological target but also as a potential therapeutic target in the treatment of these disorders.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
6 product images
![Immunoprecipitation - Anti-Phosphotyrosine antibody [EPR16871] - BSA and Azide free (ab240219), expandable thumbnail](https://content.abcam.com/products/images/phosphotyrosine-antibody-epr16871-bsa-and-azide-free-ab240219--immunoprecipitation-img46428.jpg "Immunoprecipitation - Anti-Phosphotyrosine antibody [EPR16871] - BSA and Azide free (ab240219)")
Immunoprecipitation - Anti-Phosphotyrosine antibody [EPR16871] - BSA and Azide free (ab240219)
Phosphotyrosine was immunoprecipitated from 1mg of A431 (Human epidermoid carcinoma) whole cell extract treated with 1mM pervanadate for 30 minutes with Anti-Phosphotyrosine antibody [EPR16871] ab179530 at 1/100 dilution. Western blot was performed from the immunoprecipitate using Anti-Phosphotyrosine antibody [EPR16871] ab179530 at 1/1000 dilution. Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG, was used as secondary antibody at 1/1500 dilution. Lane 1: A431 treated with 1mM pervanadate for 30 minutes whole cell extract. Lane 2: PBS instead of A431 whole cell extract.
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Multiple bands represent phosph-tyrosine containing proteins precipitated and detected by Anti-Phosphotyrosine antibody [EPR16871] ab179530.This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-Phosphotyrosine antibody [EPR16871] ab179530).
All lanes: Immunoprecipitation - Anti-Phosphotyrosine antibody [EPR16871] (Anti-Phosphotyrosine antibody [EPR16871] ab179530)
![Immunocytochemistry/ Immunofluorescence - Anti-Phosphotyrosine antibody [EPR16871] - BSA and Azide free (ab240219), expandable thumbnail](https://content.abcam.com/products/images/phosphotyrosine-antibody-epr16871-bsa-and-azide-free-ab240219--immunocytochemistry-immunofluorescence-img88372.jpg "Immunocytochemistry/ Immunofluorescence - Anti-Phosphotyrosine antibody [EPR16871] - BSA and Azide free (ab240219)")
Immunocytochemistry/ Immunofluorescence - Anti-Phosphotyrosine antibody [EPR16871] - BSA and Azide free (ab240219)
Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized C2C12 (Mouse myoblast cell line) cells labeling Phosphotyrosine with Anti-Phosphotyrosine antibody [EPR16871] ab179530 at 1/100 dilution, followed by Goat anti-rabbit IgG (Alexa Fluor® 488) (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) secondary antibody at 1/400 dilution (green). Cytoplasm staining on C2C12 cells is observed. The expression increased after treatment with H2O2 (2mM) for 10 minutes. The nuclear counterstain is DAPI (blue). Tubulin is detected with Anti-alpha Tubulin antibody [DM1A] - Loading Control ab7291 (anti-Tubulin mouse mAb) at 1/500 dilution and Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) preadsorbed ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/500 dilution (red).
The negative controls are as follows:-
-ve control 1: - Anti-Phosphotyrosine antibody [EPR16871] ab179530 at 1/100 dilution followed by Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) preadsorbed ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/500 dilution.
-ve control 2: - Anti-alpha Tubulin antibody [DM1A] - Loading Control ab7291 (anti-Tubulin mouse mAb) at 1/500 dilution followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077 (Alexa Fluor®488 Goat Anti-Rabbit IgG H&L) at 1/400 dilution.This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-Phosphotyrosine antibody [EPR16871] ab179530).
![ELISA - Anti-Phosphotyrosine antibody [EPR16871] - BSA and Azide free (ab240219), expandable thumbnail](https://content.abcam.com/products/images/phosphotyrosine-antibody-epr16871-bsa-and-azide-free-ab240219--elisa-img63318.jpg "ELISA - Anti-Phosphotyrosine antibody [EPR16871] - BSA and Azide free (ab240219)")
ELISA - Anti-Phosphotyrosine antibody [EPR16871] - BSA and Azide free (ab240219)
Serially diluted Anti-Phosphotyrosine antibody [EPR16871] ab179530 was bound to immobilised phospho- or control peptides (STAT1 (phospho S727), STAT1 control, STAT5 (phospho T694), STAT5 control); 1 microgram per mL).
The antibody was detected by Goat anti-Rabbit HRPO and signal was developed by TMB substrate.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-Phosphotyrosine antibody [EPR16871] ab179530).
![Dot Blot - Anti-Phosphotyrosine antibody [EPR16871] - BSA and Azide free (ab240219), expandable thumbnail](https://content.abcam.com/products/images/phosphotyrosine-antibody-epr16871-bsa-and-azide-free-ab240219--dot-blot-img17988.jpg "Dot Blot - Anti-Phosphotyrosine antibody [EPR16871] - BSA and Azide free (ab240219)")
Dot Blot - Anti-Phosphotyrosine antibody [EPR16871] - BSA and Azide free (ab240219)
Dot blot analysis of INSR/IGF-1R (pY1009) phospho peptide (lane 1) and INSR/IGF-1R non-phospho peptide (lane 2) labelling Phosphotyrosine with Anti-Phosphotyrosine antibody [EPR16871] ab179530 at a dilution of 1/1000. A peroxidase-conjugated goat anti-rabbit IgG (H+L) was used as the secondary antibody (1/2500).
Blocking and dilution buffer: 5% NFDM/TBST.
Exposure time: 3 minutes.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-Phosphotyrosine antibody [EPR16871] ab179530).
![Flow Cytometry (Intracellular) - Anti-Phosphotyrosine antibody [EPR16871] - BSA and Azide free (ab240219), expandable thumbnail](https://content.abcam.com/products/images/phosphotyrosine-antibody-epr16871-bsa-and-azide-free-ab240219--flow-cytometry-intracellular-img108181.jpg "Flow Cytometry (Intracellular) - Anti-Phosphotyrosine antibody [EPR16871] - BSA and Azide free (ab240219)")
Flow Cytometry (Intracellular) - Anti-Phosphotyrosine antibody [EPR16871] - BSA and Azide free (ab240219)
Intracellular flow cytometric analysis of 2% paraformaldehyde-fixed Pervanadate (50mM, 30min.) treated (orange)/untreated (red) A431 (Human epidermoid carcinoma) cells labeling Phosphotyrosine with Anti-Phosphotyrosine antibody [EPR16871] ab179530 at 1/160 dilution compared with a rabbit monoclonal IgG control (black) and an unlabelled control (cells without incubation with primary antibody and secondary antibody; blue). Goat anti rabbit IgG (FITC) at 1/150 dilution was used as the secondary antibody.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-Phosphotyrosine antibody [EPR16871] ab179530).
![ELISA - Anti-Phosphotyrosine antibody [EPR16871] - BSA and Azide free (ab240219), expandable thumbnail](https://content.abcam.com/products/images/phosphotyrosine-antibody-epr16871-bsa-and-azide-free-ab240219--elisa-img7637.jpg "ELISA - Anti-Phosphotyrosine antibody [EPR16871] - BSA and Azide free (ab240219)")
ELISA - Anti-Phosphotyrosine antibody [EPR16871] - BSA and Azide free (ab240219)
ELISA analysis of various antigens (1 µg/ml) using Anti-Phosphotyrosine antibody [EPR16871] ab179530 at 1/6400 dilution followed by Alkaline Phosphatase-conjugated Goat Anti-Rabbit IgG (H+L) at 1/2500 dilution.
S/N = signal-to-noise ratio of phospho- versus nonphospho-peptides.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-Phosphotyrosine antibody [EPR16871] ab179530).
![ELISA - Anti-Phosphotyrosine antibody [EPR16871] - BSA and Azide free (ab240219), expandable thumbnail](https://content.abcam.com/products/images/phosphotyrosine-antibody-epr16871-bsa-and-azide-free-ab240219--elisa-img7637.jpg/_jcr_content/renditions/webp-475.webp "ELISA - Anti-Phosphotyrosine antibody [EPR16871] - BSA and Azide free (ab240219)")
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