AB5592

Anti-Phosphotyrosine antibody [IG2]

Name: Anti-Phosphotyrosine antibody [IG2] (ab5592) | Abcam
Brand: Abcam Limited
SKU: AB5592
Price: 525 USD
Availability: InStock

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(2 Publications)

Mouse Monoclonal Phosphotyrosine antibody. Suitable for ICC, IP, Flow Cyt, ELISA, WB, ICC/IF and reacts with Modified Amino Acid samples. Cited in 2 publications. Immunogen corresponding to Chemical / Small Molecule corresponding to Phosphotyrosine.

2 Images

Immunocytochemistry/ Immunofluorescence - Anti-Phosphotyrosine antibody [IG2] (AB5592)

ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-Phosphotyrosine antibody [IG2] (AB5592)

Immunolocalization of phosphotyrosine in HeLa cells using ab5592.

ELISA - Anti-Phosphotyrosine antibody [IG2] (AB5592)

ELISA

Supplier Data

ELISA - Anti-Phosphotyrosine antibody [IG2] (AB5592)

Direct ELISA of a 96 well plate coated with 100 uL per well of phosphotyrosine, phosphoserine or phosphothreonine at 5 μg/mL, incubated with 100 μL per well of ab5592

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

IG2

Isotype

IgG1

Carrier free

Applications

IP, ELISA, ICC, Flow Cyt, ICC/IF, WB

applications

Specificity

ab5592 detects phosphotyrosine from most species and tissues. ab5592 will not react with any other phosphorylated amino acids, proteins or nucleotides.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICC" : {"fullname" : "Immunocytochemistry", "shortname":"ICC"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "ELISA" : {"fullname" : "ELISA", "shortname":"ELISA"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Modified Amino Acid": { "ICC-species-checked": "guaranteed", "ICC-species-dilution-info": "1/50 - 1/200", "ICC-species-notes": "", "IP-species-checked": "guaranteed", "IP-species-dilution-info": "2 µg/mL", "IP-species-notes": "", "FlowCyt-species-checked": "guaranteed", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<a href='/en-us/products/primary-antibodies/mouse-igg1-kappa-monoclonal-15-6e10a7-isotype-control-ab170190'>ab170190</a> - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.", "ELISA-species-checked": "testedAndGuaranteed", "ELISA-species-dilution-info": "1 µg/mL", "ELISA-species-notes": "", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "1/100 - 1/1000", "WB-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/50 - 1/200", "ICCIF-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

Preservative: 0.05% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine)

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Phosphotyrosine also known simply as pY is a post-translational modification where a phosphate group binds to the hydroxyl group of a tyrosine amino acid in proteins. This small modification can have a huge impact on proteins' function and interaction. Phosphotyrosine has a molecular weight of approximately 216 Da when it stands on its own. This modification can occur in various proteins expressed widely across tissues playing a role in signaling cell division and morphological changes.

Biological function summary

Phosphorylated tyrosine residues modify the function and activity of proteins within cells. It is a critical component of signal transduction pathways and can alter protein functions when becoming part of large protein complexes. It often regulates receptor proteins and intracellular kinases modifying their ability to interact with other proteins like SH2 and PTB domain-containing proteins. Phosphotyrosine antibodies like anti-phosphotyrosine Ig2 are helpful research tools for detecting these modifications.

Pathways

The modification of tyrosine to phosphotyrosine participates significantly in pathways such as the MAPK/ERK pathway and the PI3K/AKT pathway. These pathways are important for various cellular processes including growth and survival. Proteins like EGFR and PDGFR regulate signaling cascades by phosphorylation of tyrosine residues and often interact with phosphotyrosine and phosphotyrosine antibodies importantly.

The dysregulation of phosphotyrosine levels contributes to conditions like cancer and diabetes. Aberrant activation or overexpression of phosphotyrosine residues on receptors like HER2 and insulin receptors can promote uncontrolled cell proliferation often seen in tumors and contribute to insulin resistance respectively. As such phosphotyrosine serves not only as a biological target but also as a potential therapeutic target in the treatment of these disorders.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

See full target information Phosphotyrosine

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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Pharmacology research & perspectives 13:e70167 PubMed40842442

2025

6-Nitrodopamine Release From Mouse Seminal Vesicles Is Dependent on Endothelial Nitric Oxide Synthase (eNOS) Activation.

Applications

Unspecified application

Species

Unspecified reactive species

José Britto-Júnior,Pérola Rafaella Cedano Godoy,Denis Oliveira Lima,Valéria B de Souza,André A Schenka,Fernanda V Mariano,Felipe Fernandes Jacintho,Idam Hermawan,Hiroaki Shimokawa,Masato Tsutsui,Silvana Chiavegatto,Edson Antunes,Gilberto De Nucci

Nature communications 16:5875 PubMed40595687

2025

Arginine regulates the mucoid phenotype of hypervirulent Klebsiella pneumoniae.

Applications

Unspecified application

Species

Unspecified reactive species

Brooke E Ryan,Caitlyn L Holmes,Drew J Stark,Grace E Shepard,Emma G Mills,Saroj Khadka,Daria Van Tyne,Michael A Bachman,Laura A Mike

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Phosphotyrosine antibody [IG2]

Immunocytochemistry/ Immunofluorescence - Anti-Phosphotyrosine antibody [IG2] (AB5592)

ELISA - Anti-Phosphotyrosine antibody [IG2] (AB5592)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Applications

Specificity

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Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (2)

Product promise