AB190824

Anti-Phosphotyrosine antibody [RM111]

Recombinant
What is this?

Be the first to review this product! Submit a review

(4 Publications)

Rabbit Recombinant Monoclonal Phosphotyrosine antibody. Suitable for IP, ChIP, Flow Cyt, ELISA, WB, IHC-P, ICC/IF and reacts with Modified Amino Acid samples. Cited in 4 publications. Immunogen corresponding to Chemical / Small Molecule corresponding to Phosphotyrosine.

4 Images

Immunocytochemistry/ Immunofluorescence - Anti-Phosphotyrosine antibody [RM111] (AB190824)

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-Phosphotyrosine antibody [RM111] (AB190824)

Immunocytochemistry of serum-starved A431 cells nontreated (Left) or treated with EGF (Right) labeling Phosphotyrosine-EGFR using ab190824 at 1/500 dilution (followed by a PE conjugated secondary antibody, red) and DAPI (blue).

Supplier Data

Immunoprecipitation - Anti-Phosphotyrosine antibody [RM111] (AB190824)

Immunoprecipitation of EGF-treated A431 cells pulling down Phosphotyrosine-EGFR by ab190824 at 1/1000 dilution; this was followed by western blot with an anti-EGFR rabbit monoclonal antibody.
(1) Whole lysate control;

(2) IP by rabbit IgG control;

(3) IP by ab190824

All lanes:

Immunoprecipitation - Anti-Phosphotyrosine antibody [RM111] (ab190824)

false

Supplier Data

Western blot - Anti-Phosphotyrosine antibody [RM111] (AB190824)

The blot was was subjected twice to increasing exposure time in Lanes 3 and 4, and Lanes 5 and 6.

All lanes:

Western blot - Anti-Phosphotyrosine antibody [RM111] (ab190824) at 1/5000 dilution

Lanes 1, 3 and 5:

Non treated serum-starved A431 cell lysate

Lanes 2, 4 and 6:

EGF treated serum-starved A431 cell lysate

false

ELISA - Anti-Phosphotyrosine antibody [RM111] (AB190824)

ELISA

Supplier Data

ELISA - Anti-Phosphotyrosine antibody [RM111] (AB190824)

Analysis of ab190824 reaction to phosphorylated or nonphosphorylated Serine, Threonine, and Tyrosine. ab190824 only reacted to phosphorylated Tyrosine.

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

RM111

Isotype

IgG

Carrier free

Applications

ChIP, WB, ICC/IF, ELISA, IP, IHC-P, Flow Cyt

applications

Specificity

This antibody reacts to tyrosine-phosphorylated proteins. No cross reactivity with non phosphorylated tyrosine, phospho-serine, and phospho-threonine

style

left-column

style

left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "ChIP" : {"fullname" : "ChIP", "shortname":"ChIP"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "ELISA" : {"fullname" : "ELISA", "shortname":"ELISA"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Modified Amino Acid": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "1/200 - 1/1000", "IP-species-notes": "", "ChIP-species-checked": "guaranteed", "ChIP-species-dilution-info": "1/200 - 1/1000", "ChIP-species-notes": "", "FlowCyt-species-checked": "guaranteed", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<a href='/en-us/products/primary-antibodies/rabbit-igg-monoclonal-epr25a-isotype-control-ab172730'>ab172730</a> - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.", "ELISA-species-checked": "testedAndGuaranteed", "ELISA-species-dilution-info": "", "ELISA-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/5000", "WB-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "1/200 - 1/500", "IHCP-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/200 - 1/500", "ICCIF-species-notes": "" } } }

style

left-column

style

left-column

Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Purification notes

Purified from an animal origin–free culture supernatant.

Storage buffer

Preservative: 0.09% Sodium azide Constituents: PBS, 50% Glycerol (glycerin, glycerine), 1% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

style

left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Phosphotyrosine also known simply as pY is a post-translational modification where a phosphate group binds to the hydroxyl group of a tyrosine amino acid in proteins. This small modification can have a huge impact on proteins' function and interaction. Phosphotyrosine has a molecular weight of approximately 216 Da when it stands on its own. This modification can occur in various proteins expressed widely across tissues playing a role in signaling cell division and morphological changes.

Biological function summary

Phosphorylated tyrosine residues modify the function and activity of proteins within cells. It is a critical component of signal transduction pathways and can alter protein functions when becoming part of large protein complexes. It often regulates receptor proteins and intracellular kinases modifying their ability to interact with other proteins like SH2 and PTB domain-containing proteins. Phosphotyrosine antibodies like anti-phosphotyrosine Ig2 are helpful research tools for detecting these modifications.

Pathways

The modification of tyrosine to phosphotyrosine participates significantly in pathways such as the MAPK/ERK pathway and the PI3K/AKT pathway. These pathways are important for various cellular processes including growth and survival. Proteins like EGFR and PDGFR regulate signaling cascades by phosphorylation of tyrosine residues and often interact with phosphotyrosine and phosphotyrosine antibodies importantly.

The dysregulation of phosphotyrosine levels contributes to conditions like cancer and diabetes. Aberrant activation or overexpression of phosphotyrosine residues on receptors like HER2 and insulin receptors can promote uncontrolled cell proliferation often seen in tumors and contribute to insulin resistance respectively. As such phosphotyrosine serves not only as a biological target but also as a potential therapeutic target in the treatment of these disorders.

style

left-column

style

left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

style

left-column

Target data

See full target information Phosphotyrosine

style

left-column

Publications (4)

Recent publications for all applications. Explore the full list and refine your search

The New phytologist 237:471-482 PubMed36266960

2022

Natural variation of ZmLNG1 alters organ shapes in maize.

Applications

Unspecified application

Species

Unspecified reactive species

Qin Wang,Jiongjiong Fan,Jia Cong,Mengjiao Chen,Jie Qiu,Jie Liu,Xiaoyan Zhao,Ruipeng Huang,Hongjun Liu,Xuehui Huang

Experimental and therapeutic medicine 24:480 PubMed35761813

2022

Vitamin E-coated dialyzer alleviates erythrocyte deformability dysfunction in patients with end-stage renal disease undergoing hemodialysis.

Applications

Unspecified application

Species

Unspecified reactive species

Ying Zhang,Wei Gao,Xia Li

Frontiers in physiology 12:712400 PubMed34504438

2021

Regulates the Proliferation and Migration of Vascular Smooth Muscle Cells in the Restenosis Process Post-PTA in Lower Extremity Arteries.

Applications

Unspecified application

Species

Unspecified reactive species

Mi Zhou,Lixing Qi,Yongquan Gu

The Journal of biological chemistry 294:15577-15592 PubMed31439665

2019

17β-Estradiol nongenomically induces vascular endothelial HS release by promoting phosphorylation of cystathionine γ-lyase.

Applications

Unspecified application

Species

Unspecified reactive species

Xingyan Xu,Qing Yan,Xiaoyun Liu,Ping Li,Xiaosa Li,Yiwen Chen,Tommaso Simoncini,Junxiu Liu,Dongxing Zhu,Xiaodong Fu

View all publications

style

left-column

style

left-column

style

right-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Phosphotyrosine antibody [RM111]

Immunocytochemistry/ Immunofluorescence - Anti-Phosphotyrosine antibody [RM111] (AB190824)

Immunoprecipitation - Anti-Phosphotyrosine antibody [RM111] (AB190824)

Western blot - Anti-Phosphotyrosine antibody [RM111] (AB190824)

ELISA - Anti-Phosphotyrosine antibody [RM111] (AB190824)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Applications

Specificity

Alternative Products

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Complementary Products

Assay Kits

Secondary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (4)

Product promise