AB249179

Anti-PHOX2A antibody [EPR9071] - BSA and Azide free

RabMAb
Recombinant
What is this?

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(1 Publication)

Rabbit Recombinant Monoclonal PHOX2A antibody. Carrier free. Suitable for ICC/IF, WB and reacts with Human, Mouse samples. Cited in 1 publication.

View Alternative Names

ARIX, PMX2A, PHOX2A, Paired mesoderm homeobox protein 2A, ARIX1 homeodomain protein, Aristaless homeobox protein homolog, Paired-like homeobox 2A

3 Images

Lab

Western blot - Anti-PHOX2A antibody [EPR9071] - BSA and Azide free (AB249179)

This data was developed using ab155084, the same antibody clone in a different buffer formulation. Blocking and diluting buffer and concentration : 5% NFDM /TBST. ab181602 was used as a GAPDH loading control. Exposure time : Lane 1-3 : 180 seconds; Lane 4 : 40 seconds. HEK293 and HeLa are negative controls (PMID : 17505528, PMID : 17344216). The expression profile/ molecular weight observed is consistent with what has been described in the literature (PMID : 17344216)

All lanes:

Western blot - Anti-PHOX2A antibody [EPR9071] (<a href='/en-us/products/primary-antibodies/phox2a-antibody-epr9071-ab155084'>ab155084</a>) at 1/1000 dilution

Lane 1:

HEK293 (Human embryonic kidney epithelial cell) whole cell lysate at 20 µg

Lane 2:

HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysate at 20 µg

Lane 3:

SH-SY5Y (Human neuroblastoma epithelial cell) whole cell lysate at 20 µg

Lane 4:

IMR-32 (Human neuroblastoma neuroblast) whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Predicted band size: 30 kDa

false

Immunocytochemistry/ Immunofluorescence - Anti-PHOX2A antibody [EPR9071] - BSA and Azide free (AB249179)

ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-PHOX2A antibody [EPR9071] - BSA and Azide free (AB249179)

This data was developed using ab155084, the same antibody clone in a different buffer formulation.Immunofluorescent analysis of SH-SY-5Y cells, labeling PHOX2A with ab155084 at 1/100 dilution.

Unknown

Western blot - Anti-PHOX2A antibody [EPR9071] - BSA and Azide free (AB249179)

This data was developed using ab155084, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-PHOX2A antibody [EPR9071] (<a href='/en-us/products/primary-antibodies/phox2a-antibody-epr9071-ab155084'>ab155084</a>) at 1/1000 dilution

Lane 1:

SH-SY-5Y cell lysate at 10 µg

Lane 2:

Neuro-2a cell lysate at 10 µg

Secondary

All lanes:

Goat anti-rabbit HRP at 1/2000 dilution

Predicted band size: 30 kDa

false

Unconjugated

Anti-PHOX2A antibody [EPR9071]

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR9071

Isotype

IgG

Carrier free

Yes

Reacts with

Mouse, Human

Applications

ICC/IF, WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Mouse": { "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }

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Product details

ab249179 is the carrier-free version of ab155084.

Species reactivity
Rat: We have preliminary internal testing data to indicate this antibody may not react with this species.
Please contact us for more information.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar^® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar^® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification

Storage buffer

pH: 7.2 - 7.4 Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Storage information

Do Not Freeze

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

PHOX2A also known as Arix among other names is a transcription factor with an approximate mass of 34 kDa. It plays an essential role in the development of the autonomic nervous system. PHOX2A shows specific expression in neuronal tissues particularly in the noradrenergic and cholinergic neurons. Its function involves the regulation of genes that control neuronal differentiation and development.

Biological function summary

PHOX2A is key in the regulation of noradrenergic phenotype important for the formation and maintenance of the autonomic nervous system. It interacts as part of a transcriptional complex working directly to activate the expression of genes necessary for the synthesis of catecholamines such as dopamine beta-hydroxylase. The protein ensures the proper differentiation of neural crest cells into sympathetic and parasympathetic neurons by influencing its genetic targets.

Pathways

PHOX2A is integral to the development of the autonomic nervous system pathway and the noradrenergic biosynthesis pathway. Much of its function is orchestrated through interactions with other transcription factors like PHOX2B. By regulating gene expression within these pathways PHOX2A contributes to the broader network of neural crest cell development impacting cellular processes that underpin nervous system formation and function.

Mutations in PHOX2A have been linked to congenital central hypoventilation syndrome and Hirschsprung disease. In these conditions PHOX2A's relationships with other proteins notably PHOX2B are significant as these proteins share overlapping roles in neural development. Disruptions in PHOX2A consequently impair pathways and processes critical for autonomic control and gut innervation leading to the aforementioned disorders.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

May be involved in regulating the specificity of expression of the catecholamine biosynthetic genes. Acts as a transcription activator/factor. Could maintain the noradrenergic phenotype.

See full target information PHOX2A

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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

International journal of clinical and experimental 13:2973-2983 PubMed33425098

2020

Adsorption of miR-218 by lncRNA HOTAIR regulates PDE7A and affects glioma cell proliferation, invasion, and apoptosis.

Applications

Unspecified application

Species

Unspecified reactive species

Yigong Wei,Kun Zhou,Cheng Wang,Xiaolin Du,Qing Xiao,Changyi Chen

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-PHOX2A antibody [EPR9071] - BSA and Azide free

Western blot - Anti-PHOX2A antibody [EPR9071] - BSA and Azide free (AB249179)

Immunocytochemistry/ Immunofluorescence - Anti-PHOX2A antibody [EPR9071] - BSA and Azide free (AB249179)

Western blot - Anti-PHOX2A antibody [EPR9071] - BSA and Azide free (AB249179)

Related conjugates and formulations (1)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (1)

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