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Anti-PHOX2B antibody [EPR14423] - C-terminal (ab183741) is a rabbit monoclonal antibody that is used to detect PHOX2B in Western Blot, IHC-P, ICC/IF. Suitable for Human, Mouse samples.



- Using biophysical QC, antibody identity is confirmed at a molecular level for unrivalled batch-batch consistency

-Over 10 publications


Images

Immunocytochemistry/ Immunofluorescence - Anti-PHOX2B antibody [EPR14423] - C-terminal (AB183741), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PHOX2B antibody [EPR14423] - C-terminal (AB183741), expandable thumbnail
  • Western blot - Anti-PHOX2B antibody [EPR14423] - C-terminal (AB183741), expandable thumbnail
  • Immunocytochemistry/ Immunofluorescence - Anti-PHOX2B antibody [EPR14423] - C-terminal (AB183741), expandable thumbnail
  • Immunocytochemistry/ Immunofluorescence - Anti-PHOX2B antibody [EPR14423] - C-terminal (AB183741), expandable thumbnail

Publications

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
ICC/IFWBIHC-P
Human
Tested
Expected
Tested
Mouse
Expected
Tested
Expected

Tested
Tested

Species
Human
Dilution info
1/100
Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Expected
Expected

Species
Mouse
Dilution info
Use at an assay dependent concentration.
Notes

-

Tested
Tested

Species
Mouse
Dilution info
1/1000 - 1/2000
Notes

-

Expected
Expected

Species
Human
Dilution info
Use at an assay dependent concentration.
Notes

-

Tested
Tested

Species
Human
Dilution info
1/1000
Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Expected
Expected

Species
Mouse
Dilution info
Use at an assay dependent concentration.
Notes

-

Associated Products

Select an associated product type

1 product for Alternative Product

Target data

Function

Involved in the development of several major noradrenergic neuron populations, including the locus coeruleus. Transcription factor which could determine a neurotransmitter phenotype in vertebrates. Enhances second-messenger-mediated activation of the dopamine beta-hydrolase and c-fos promoters, and of several enhancers including cAMP-response element and serum-response element.

Alternative names

PMX2B, PHOX2B, Paired mesoderm homeobox protein 2B, Neuroblastoma Phox, PHOX2B homeodomain protein, Paired-like homeobox 2B, NBPhox

Recommended products

Anti-PHOX2B antibody [EPR14423] - C-terminal (ab183741) is a rabbit monoclonal antibody that is used to detect PHOX2B in Western Blot, IHC-P, ICC/IF. Suitable for Human, Mouse samples.



- Using biophysical QC, antibody identity is confirmed at a molecular level for unrivalled batch-batch consistency

-Over 10 publications

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number
EPR14423
Purification technique
Affinity purification Protein A
Specificity

The mouse recommendation is based on the WB results. We do not guarantee IHC-P for mouse.

Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle

Notes

What is this antibody validated in?


Anti-PHOX2B antibody [EPR14423] - C-terminal (ab183741) is a rabbit recombinant monoclonal antibody and is validated for use in Western Blot (WB), Immunohistochemistry (IHC-P), Immunocytochemistry/immunofluorescence (ICC/IF), in Human, Mouse samples.

What is the molecular weight of PHOX2B?


Anti-PHOX2B [EPR14423] - C-terminal (ab183741) specifically detects a band for PHOX2B (UniProt: Q99453) at a molecular weight of 32kDa.

Recommended positive controls


WB: Neuro-2a and SH-SY5Y cell lysate.IHC-P: Human neuroblastoma tissue.ICC/IF: HeLa, Neuro-2a and SH-SY5Y cells.

Trusted by the scientific community


Anti-PHOX2B [EPR14423] - C-terminal (ab183741) was first used in a scientific publication in 2014 and has been cited over 10 times in peer-reviewed journals.

Trial sizes available!


Test your antibody or perform pre-screening before committing to a larger quantity. Sold in 10µl. Discover our selection of trial-size antibodies.Other related products


We have a range of other formats of antibody clone [EPR14423] also available for your convenience:
ab183741, Alexa Fluor® 488 - Alexa Fluor® 488 Anti-PHOX2B antibody [EPR14423] - C-terminal ab210459, Alexa Fluor® 594 - Alexa Fluor® 594 Anti-PHOX2B antibody [EPR14423] - C-terminal ab210658, Carrier free - Anti-PHOX2B antibody [EPR14423] - BSA and Azide free ab216456, Alexa Fluor® 647 - Alexa Fluor® 647 Anti-PHOX2B antibody [EPR14423] - C-terminal ab311130, Alexa Fluor® 568 - Alexa Fluor® 568 Anti-PHOX2B antibody [EPR14423] - C-terminal ab313043, Alexa Fluor® 555 - Alexa Fluor® 555 Anti-PHOX2B antibody [EPR14423] - C-terminal ab313244, Alexa Fluor® 750 - Alexa Fluor® 750 Anti-PHOX2B antibody [EPR14423] - C-terminal ab321283



Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

PHOX2B also known as Paired-like Homeobox 2B is a transcription factor that plays a significant role in neural development. It is encoded by the PHOX2B gene and has a molecular weight of approximately 31 kDa. This protein is highly expressed in the central and peripheral nervous systems where it influences the differentiation and function of neural crest-derived cells. PHOX2B is particularly active in the brainstem regions that control vital autonomic functions.

Biological function summary

PHOX2B influences the development of noradrenergic neurons and parasympathetic ganglia. It often functions as part of a transcription factor complex interacting with other proteins to drive the expression of genes required for the autonomic nervous system. The control PHOX2B exerts over neuronal differentiation makes it essential for maintaining the proper functioning of neurons responsible for involuntary bodily processes.

Pathways

PHOX2B is integral to the development and maintenance of the autonomic nervous and respiratory systems. It interacts closely with RET a receptor tyrosine kinase within the neurotrophic signaling pathways that regulate neuronal growth and survival. Additionally the protein plays a role in the endothelin signaling pathway further demonstrating its importance in neurodevelopment.

Associated diseases and disorders

Alterations in PHOX2B have been linked to disorders such as congenital central hypoventilation syndrome (CCHS) and neuroblastoma. CCHS results from mutations in the PHOX2B gene affecting respiratory control. In the context of cancer PHOX2B neuroblastoma pertains to its potential role in tumor formation alongside MYCN amplification which is a common alteration in neuroblastoma. Understanding PHOX2B’s interactions provides insights into its potential as a target for therapeutic interventions.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

6 product images

  • Immunocytochemistry/ Immunofluorescence - Anti-PHOX2B antibody [EPR14423] - C-terminal (ab183741), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-PHOX2B antibody [EPR14423] - C-terminal (ab183741)

    Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized Neuro-2a (mouse neuroblastoma neuroblast) cell line labelling PHOX2B with ab183741 at 1/100 (10 μg/ml) dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) antibody at 1/1000 dilution (Green).

    Confocal image showing nuclear staining in Neuro-2a cell line.

    Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 dilution (Red). The nuclear counterstain was DAPI (Blue).

    Secondary antibody only control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) at 1/1000 dilution.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PHOX2B antibody [EPR14423] - C-terminal (ab183741), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PHOX2B antibody [EPR14423] - C-terminal (ab183741)

    Immunohistochemical analysis of paraffin-embedded Human neuroblastoma tissue labeling PHOX2B with ab183741 at 1/1000 dilution. The slide is counterstained with Hematoxylin.

    Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.

  • Western blot - Anti-PHOX2B antibody [EPR14423] - C-terminal (ab183741), expandable thumbnail

    Western blot - Anti-PHOX2B antibody [EPR14423] - C-terminal (ab183741)

    All lanes: Western blot - Anti-PHOX2B antibody [EPR14423] - C-terminal (ab183741) at 1/1000 dilution

    Lane 1: Neuro-2a cell lysate at 20 µg

    Lane 2: SH-SY5Y cell lysate at 20 µg

    Secondary

    All lanes: Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 32 kDa

  • Immunocytochemistry/ Immunofluorescence - Anti-PHOX2B antibody [EPR14423] - C-terminal (ab183741), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-PHOX2B antibody [EPR14423] - C-terminal (ab183741)

    Immunofluorescence analysis of acetone-fixed HeLa cells labeling PHOX2B with ab183741 at 1/100 dilution (red). Goat anti-rabbit IgG (Alexa Fluor® 555) at 1/200 dilution was used as the secondary antibody. The slide on the right is stained with Dapi (blue).

  • Immunocytochemistry/ Immunofluorescence - Anti-PHOX2B antibody [EPR14423] - C-terminal (ab183741), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-PHOX2B antibody [EPR14423] - C-terminal (ab183741)

    Immunofluorescence analysis of 4% paraformaldehyde-fixed SH-SY5Y cells labeling PHOX2B with ab183741 at 1/100 dilution (red). Goat anti-rabbit IgG (Alexa Fluor® 555) at 1/200 dilution was used as the secondary antibody. The slide on the right is stained with Dapi (blue).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PHOX2B antibody [EPR14423] - C-terminal (ab183741), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PHOX2B antibody [EPR14423] - C-terminal (ab183741)

    PHOX2B Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) staining of Human adrenal tissue using rabbit Anti-PHOX2B antibody

    Immunohistochemical analysis of formalin fixed paraffin embedded human adrenal labelling PHOX2B with ab183741 at a concentration of 3μg/ml. The immunostaining was performed on a Ventana DISCOVERY ULTRA (Roche Tissue Diagnostics) instrument with a OptiView DAB IHC Detection Kit. Heat mediated antigen retrieval was performed with DISCOVERY cell conditioning solution (CC1) 100°C, pH8.5 for 32mins.

    ab183741 Anti-PHOX2B antibody [EPR14423] antibody was incubated for 16mins at 37°C. Sections were counterstained with Hematoxylin II. Image inset shows absence of staining in secondary antibody only control.

    Customers are encouraged to optimise antigen retrieval conditions, antibody concentration, incubation times and temperature for best results in their own IHC assay workflow (automated and manual).

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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