AB227719

Anti-PHOX2B antibody - N-terminal

Name: Anti-PHOX2B antibody - N-terminal (ab227719) | Abcam
Brand: Abcam Limited
SKU: AB227719
Price: 525 USD
Availability: InStock

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(1 Publication)

Rabbit Polyclonal PHOX2B antibody. N-terminal. Suitable for IP, WB and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Synthetic Peptide within Human PHOX2B.

View Alternative Names

PMX2B, PHOX2B, Paired mesoderm homeobox protein 2B, Neuroblastoma Phox, PHOX2B homeodomain protein, Paired-like homeobox 2B, NBPhox

3 Images

Supplier Data

Western blot - Anti-PHOX2B antibody - N-terminal (AB227719)

12% SDS-PAGE gel.

All lanes:

Western blot - Anti-PHOX2B antibody - N-terminal (ab227719) at 1/1000 dilution

Lane 1:

IMR32 (human neuroblast cell line) whole cell extract at 30 µg

Lane 2:

SK-N-AS (human neuroblastoma cell line) whole cell extract at 30 µg

Predicted band size: 32 kDa

false

Supplier Data

Western blot - Anti-PHOX2B antibody - N-terminal (AB227719)

12% SDS-PAGE gel.

All lanes:

Western blot - Anti-PHOX2B antibody - N-terminal (ab227719) at 1/1000 dilution

All lanes:

SH-SY5Y (human neuroblastoma cell line from bone marrow) whole cell extract at 30 µg

Predicted band size: 32 kDa

false

Supplier Data

Immunoprecipitation - Anti-PHOX2B antibody - N-terminal (AB227719)

PHOX2B was immunoprecipitated from IMR32 (human neuroblast cell line) whole cell extract with 5 μg ab227719. Western blot was performed from the immunoprecipitate using ab227719. Anti-Rabbit IgG was used as a secondary reagent.

Lane 1 : Control IgG IP in IMR32 whole cell extract.

Lane 2 : ab227719 IP in IMR32 whole cell extract.

All lanes:

Immunoprecipitation - Anti-PHOX2B antibody - N-terminal (ab227719)

Predicted band size: 32 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

WB, IP

applications

Immunogen

Synthetic Peptide within Human PHOX2B. The exact immunogen used to generate this antibody is proprietary information.

Q99453

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "1/100 - 1/500", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "<p></p>" }, "Mouse": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Xenopus laevis": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7 Preservative: 0.025% Proclin 300 Constituents: 79% PBS, 20% Glycerol (glycerin, glycerine)

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

PHOX2B also known as Paired-like Homeobox 2B is a transcription factor that plays a significant role in neural development. It is encoded by the PHOX2B gene and has a molecular weight of approximately 31 kDa. This protein is highly expressed in the central and peripheral nervous systems where it influences the differentiation and function of neural crest-derived cells. PHOX2B is particularly active in the brainstem regions that control vital autonomic functions.

Biological function summary

PHOX2B influences the development of noradrenergic neurons and parasympathetic ganglia. It often functions as part of a transcription factor complex interacting with other proteins to drive the expression of genes required for the autonomic nervous system. The control PHOX2B exerts over neuronal differentiation makes it essential for maintaining the proper functioning of neurons responsible for involuntary bodily processes.

Pathways

PHOX2B is integral to the development and maintenance of the autonomic nervous and respiratory systems. It interacts closely with RET a receptor tyrosine kinase within the neurotrophic signaling pathways that regulate neuronal growth and survival. Additionally the protein plays a role in the endothelin signaling pathway further demonstrating its importance in neurodevelopment.

Alterations in PHOX2B have been linked to disorders such as congenital central hypoventilation syndrome (CCHS) and neuroblastoma. CCHS results from mutations in the PHOX2B gene affecting respiratory control. In the context of cancer PHOX2B neuroblastoma pertains to its potential role in tumor formation alongside MYCN amplification which is a common alteration in neuroblastoma. Understanding PHOX2B’s interactions provides insights into its potential as a target for therapeutic interventions.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Involved in the development of several major noradrenergic neuron populations, including the locus coeruleus. Transcription factor which could determine a neurotransmitter phenotype in vertebrates. Enhances second-messenger-mediated activation of the dopamine beta-hydrolase and c-fos promoters, and of several enhancers including cAMP-response element and serum-response element.

See full target information PHOX2B

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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Molecular therapy oncolytics 23:181-191 PubMed34729395

2021

Therapeutically targeting oncogenic CRCs facilitates induced differentiation of NB by RA and the BET bromodomain inhibitor.

Applications

Unspecified application

Species

Unspecified reactive species

Satyanarayana Alleboina,Nour Aljouda,Mellessa Miller,Kevin W Freeman

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-PHOX2B antibody - N-terminal

Western blot - Anti-PHOX2B antibody - N-terminal (AB227719)

Western blot - Anti-PHOX2B antibody - N-terminal (AB227719)

Immunoprecipitation - Anti-PHOX2B antibody - N-terminal (AB227719)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (1)

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