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AB276060

Anti-PHPT1 antibody [001] - BSA and Azide free

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Rabbit Recombinant Monoclonal PHPT1 antibody. Carrier free. Suitable for ICC, IHC-P, Flow Cyt (Intra) and reacts with Human samples. Immunogen corresponding to Recombinant Fragment Protein within Human PHPT1 aa 1 to C-terminus.

View Alternative Names

PHP14, CGI-202, HSPC141, PHPT1, 14 kDa phosphohistidine phosphatase, Phosphohistidine phosphatase 1, Protein histidine phosphatase, Protein janus-A homolog, PHP

3 Images
Flow Cytometry (Intracellular) - Anti-PHPT1 antibody [001] - BSA and Azide free (AB276060)
  • Flow Cyt (Intra)

Unknown

Flow Cytometry (Intracellular) - Anti-PHPT1 antibody [001] - BSA and Azide free (AB276060)

Intracellular flow cytometric analysis of Human PHPT1 expression on THP1 cells. The cells were treated according to manufacturers manuall, stained with ab276060, then a FITC-conjugated secondary antibody (black line) compared with a negative control (grey line). The Fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of intact cells.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PHPT1 antibody [001] - BSA and Azide free (AB276060)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PHPT1 antibody [001] - BSA and Azide free (AB276060)

Immunochemical staining of human PHPT1 in human skeletal muscle with ab276060 at 1/5000 dilution, formalin-fixed paraffin embedded sections.

Immunocytochemistry - Anti-PHPT1 antibody [001] - BSA and Azide free (AB276060)
  • ICC

Unknown

Immunocytochemistry - Anti-PHPT1 antibody [001] - BSA and Azide free (AB276060)

Immunofluorescence staining of Human PHPT1 in HeLa cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS, blocked with 10% serum, and incubated with ab276060 at 1/60 dilution at 37°C 1 hour. Then cells were stained with the Alexa Fluor® 488-conjugated Goat Anti-rabbit IgG secondary antibody (green) and counterstained with DAPI (blue). Positive staining was localized to cytoplasm.

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

001

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

IHC-P, Flow Cyt (Intra), ICC

applications

Immunogen

Recombinant Fragment Protein within Human PHPT1 aa 1 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

Q9NRX4

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICC" : {"fullname" : "Immunocytochemistry", "shortname":"ICC"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICC-species-checked": "testedAndGuaranteed", "ICC-species-dilution-info": "1/20 - 1/100", "ICC-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/2500 - 1/10000", "IHCP-species-notes": "<p></p>", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "1/25 - 1/100", "FlowCytIntra-species-notes": "<p></p>" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The target protein PHPT1 also known as phosphohistidine phosphatase 1 plays a mechanical role by removing phosphate groups from phosphohistidine residues in proteins. This dephosphorylation activity is essential for its function and interaction with other proteins. PHPT1 has a molecular mass of approximately 14 kDa. Scientists have observed that it is expressed in several tissues including the liver kidney and skeletal muscle indicating its importance in varied physiological contexts.
Biological function summary

This protein modulates signal transduction pathways by dephosphorylating proteins which can affect cellular signals and functions. Although not part of a larger protein complex PHPT1 acts by influencing histidine phosphorylation status therefore impacting cellular communication. The regulation of phosphorylation states by PHPT1 affects processes like growth differentiation and metabolism underlining its significance in cellular functions.

Pathways

PHPT1 engages in histidine phosphorylation pathways a lesser-known aspect compared to serine threonine and tyrosine phosphorylations. It plays a significant role in cellular signaling networks where related proteins include kinases that add phosphate groups to histidine residues. By modifying the phosphorylation state of certain proteins PHPT1 influences important pathways such as those involving signaling cascades that regulate cell growth and apoptosis.

Research links PHPT1 to cancer and metabolic disorders. In cancer altered activity or expression of PHPT1 can disrupt normal signaling pathways leading to unregulated cell growth. It connects to proteins such as histidine kinases which modify activation states of proteins involved in cell proliferation. In metabolic disorders PHPT1 dysfunction may contribute to impaired metabolic regulation through inappropriate signaling influencing pathways related to energy balance and storage.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Exhibits phosphohistidine phosphatase activity.
See full target information PHPT1
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Product promise

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