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AB276061

Anti-PHPT1 antibody [007]

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Rabbit Recombinant Monoclonal PHPT1 antibody. Carrier free. Suitable for IP, WB and reacts with Human samples. Immunogen corresponding to Recombinant Fragment Protein within Human PHPT1 aa 1 to C-terminus.

View Alternative Names

PHP14, CGI-202, HSPC141, PHPT1, 14 kDa phosphohistidine phosphatase, Phosphohistidine phosphatase 1, Protein histidine phosphatase, Protein janus-A homolog, PHP

2 Images
Immunoprecipitation - Anti-PHPT1 antibody [007] (AB276061)
  • IP

Unknown

Immunoprecipitation - Anti-PHPT1 antibody [007] (AB276061)

PHPT1 was immunoprecipitated from 0.5 mg of HeLa whole cell lysate using ab276061 at 1/100 dilution followed by Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Develope dusing the ECL technique.

All lanes:

Immunoprecipitation - Anti-PHPT1 antibody [007] (ab276061)

Predicted band size: 14 kDa

false

Western blot - Anti-PHPT1 antibody [007] (AB276061)
  • WB

Unknown

Western blot - Anti-PHPT1 antibody [007] (AB276061)

Performed under reducing conditions.

All lanes:

Western blot - Anti-PHPT1 antibody [007] (ab276061) at 1/500 dilution

Lane 1:

HepG2 whole cell lysate at 30 µg

Lane 2:

THP-1 whole cell lysate at 30 µg

Lane 3:

MCF-7 whole cell lysate at 30 µg

Secondary

All lanes:

Goat Anti- Rabbit IgG H&L (Dylight 800) at 1/10000 dilution

Predicted band size: 14 kDa

false

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

007

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

IP, WB

applications

Immunogen

Recombinant Fragment Protein within Human PHPT1 aa 1 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

Q9NRX4

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "1-5 µg/mL", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/2000", "WB-species-notes": "<p></p>" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The target protein PHPT1 also known as phosphohistidine phosphatase 1 plays a mechanical role by removing phosphate groups from phosphohistidine residues in proteins. This dephosphorylation activity is essential for its function and interaction with other proteins. PHPT1 has a molecular mass of approximately 14 kDa. Scientists have observed that it is expressed in several tissues including the liver kidney and skeletal muscle indicating its importance in varied physiological contexts.
Biological function summary

This protein modulates signal transduction pathways by dephosphorylating proteins which can affect cellular signals and functions. Although not part of a larger protein complex PHPT1 acts by influencing histidine phosphorylation status therefore impacting cellular communication. The regulation of phosphorylation states by PHPT1 affects processes like growth differentiation and metabolism underlining its significance in cellular functions.

Pathways

PHPT1 engages in histidine phosphorylation pathways a lesser-known aspect compared to serine threonine and tyrosine phosphorylations. It plays a significant role in cellular signaling networks where related proteins include kinases that add phosphate groups to histidine residues. By modifying the phosphorylation state of certain proteins PHPT1 influences important pathways such as those involving signaling cascades that regulate cell growth and apoptosis.

Research links PHPT1 to cancer and metabolic disorders. In cancer altered activity or expression of PHPT1 can disrupt normal signaling pathways leading to unregulated cell growth. It connects to proteins such as histidine kinases which modify activation states of proteins involved in cell proliferation. In metabolic disorders PHPT1 dysfunction may contribute to impaired metabolic regulation through inappropriate signaling influencing pathways related to energy balance and storage.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Exhibits phosphohistidine phosphatase activity.
See full target information PHPT1
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Product promise

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