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AB231908

Anti-PHPT1 antibody

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(1 Publication)

Rabbit Polyclonal PHPT1 antibody. Suitable for WB, IHC-P and reacts with Mouse, Rat samples. Cited in 1 publication. Immunogen corresponding to Recombinant Full Length Protein corresponding to Mouse Phpt1.

View Alternative Names

Php14, Phpt1, 14 kDa phosphohistidine phosphatase, Phosphohistidine phosphatase 1, Protein histidine phosphatase, PHPT1, PHP

4 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PHPT1 antibody (AB231908)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PHPT1 antibody (AB231908)

Formalin-fixed, paraffin-embedded mouse brain tissue stained for PHPT1 using ab231908 at 20 μg/ml in immunohistochemical analysis. DAB staining.

Western blot - Anti-PHPT1 antibody (AB231908)
  • WB

Supplier Data

Western blot - Anti-PHPT1 antibody (AB231908)

All lanes:

Western blot - Anti-PHPT1 antibody (ab231908) at 2 µg/mL

All lanes:

Mouse cerebrum lysate

Predicted band size: 14 kDa

false

Western blot - Anti-PHPT1 antibody (AB231908)
  • WB

Supplier Data

Western blot - Anti-PHPT1 antibody (AB231908)

All lanes:

Western blot - Anti-PHPT1 antibody (ab231908) at 2 µg/mL

All lanes:

Recombinant mouse PHPT1 protein

Predicted band size: 14 kDa

false

Western blot - Anti-PHPT1 antibody (AB231908)
  • WB

Supplier Data

Western blot - Anti-PHPT1 antibody (AB231908)

All lanes:

Western blot - Anti-PHPT1 antibody (ab231908) at 1 µg/mL

All lanes:

Rat testis lysate

Predicted band size: 14 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Mouse, Rat

Applications

WB, IHC-P

applications

Immunogen

Recombinant Full Length Protein corresponding to Mouse Phpt1.

Q9DAK9

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.2-2 µg/mL", "WB-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "5-20 µg/mL", "IHCP-species-notes": "<p></p>" }, "Rat": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.2-2 µg/mL", "WB-species-notes": "<p></p>", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Cow": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Purification notes
ab231908 was purified by antigen-specific affinity chromatography followed by Protein A affinity chromatography.
Storage buffer
pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 55.77% Glycerol (glycerin, glycerine)
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The target protein PHPT1 also known as phosphohistidine phosphatase 1 plays a mechanical role by removing phosphate groups from phosphohistidine residues in proteins. This dephosphorylation activity is essential for its function and interaction with other proteins. PHPT1 has a molecular mass of approximately 14 kDa. Scientists have observed that it is expressed in several tissues including the liver kidney and skeletal muscle indicating its importance in varied physiological contexts.
Biological function summary

This protein modulates signal transduction pathways by dephosphorylating proteins which can affect cellular signals and functions. Although not part of a larger protein complex PHPT1 acts by influencing histidine phosphorylation status therefore impacting cellular communication. The regulation of phosphorylation states by PHPT1 affects processes like growth differentiation and metabolism underlining its significance in cellular functions.

Pathways

PHPT1 engages in histidine phosphorylation pathways a lesser-known aspect compared to serine threonine and tyrosine phosphorylations. It plays a significant role in cellular signaling networks where related proteins include kinases that add phosphate groups to histidine residues. By modifying the phosphorylation state of certain proteins PHPT1 influences important pathways such as those involving signaling cascades that regulate cell growth and apoptosis.

Research links PHPT1 to cancer and metabolic disorders. In cancer altered activity or expression of PHPT1 can disrupt normal signaling pathways leading to unregulated cell growth. It connects to proteins such as histidine kinases which modify activation states of proteins involved in cell proliferation. In metabolic disorders PHPT1 dysfunction may contribute to impaired metabolic regulation through inappropriate signaling influencing pathways related to energy balance and storage.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Exhibits phosphohistidine phosphatase activity.
See full target information Phpt1
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

International journal of molecular sciences 26: PubMed41009702

2025

Mechanism of Regulation of NaCl Homeostasis in the Distal Colon During Obesity.

Applications

Unspecified application

Species

Unspecified reactive species

Balasubramanian Palaniappan,John Crutchley,Raja Singh Paulraj,Alip Borthakur,Subha Arthur
View all publications
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