AB85974

Anti-PHRF1 antibody

Name: Anti-PHRF1 antibody (ab85974) | Abcam
Brand: Abcam Limited
SKU: AB85974
Price: 510 USD
Availability: InStock

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(3 Publications)

Rabbit Polyclonal PHRF1 antibody. Suitable for IP, WB and reacts with Human, Mouse samples. Cited in 3 publications. Immunogen corresponding to Synthetic Peptide within Human PHRF1 aa 1400-1450.

View Alternative Names

KIAA1542, PHRF1, PHD and RING finger domain-containing protein 1

2 Images

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Immunoprecipitation - Anti-PHRF1 antibody (AB85974)

Detection of PHRF1 in Immunoprecipitates of HeLa whole cell lysate (1 mg for IP, 20% of IP loaded) using ab85974 at 3 µg/mg lysate for IP and at 1 µg/ml for subsequent Western blot detection. Detection : Chemiluminescence with an exposure time of 10 seconds.

All lanes:

Immunoprecipitation - Anti-PHRF1 antibody (ab85974)

Predicted band size: 179 kDa

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Western blot - Anti-PHRF1 antibody (AB85974)

All lanes:

Western blot - Anti-PHRF1 antibody (ab85974) at 0.04 µg/mL

Lane 1:

HeLa whole cell lysate at 50 µg

Lane 2:

HeLa whole cell lysate at 15 µg

Lane 3:

HeLa whole cell lysate at 5 µg

Lane 4:

293T whole cell lysate at 50 µg

Lane 5:

NIH3T3 whole cell lysate at 50 µg

Predicted band size: 179 kDa

Observed band size: 179 kDa

true

Exposure time: 10s

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Mouse, Human

Applications

IP, WB

applications

Immunogen

Synthetic Peptide within Human PHRF1 aa 1400-1450. The exact immunogen used to generate this antibody is proprietary information.

Q9P1Y6

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "2-5 µg/mg of lysate", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/2000 - 1/10000", "WB-species-notes": "" }, "Mouse": { "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/2000 - 1/10000", "WB-species-notes": "" }, "Chimpanzee": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Gorilla": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 6.8 - 7.4 Preservative: 0.09% Sodium azide Constituents: Tris buffered saline, 0.1% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

PHRF1 also known as PHRF1 actin binding protein functions as an E3 ubiquitin ligase within cells. It has a molecular mass of approximately 91 kDa and is widely expressed across various tissues with higher expression levels observed in the nucleus. PHRF1 interacts with and modifies other proteins through ubiquitination which signals them for degradation or alters their activity within the cell.

Biological function summary

This protein influences cellular processes by interacting with transcriptional co-repressors and partners in transcriptional repression. PHRF1 plays a significant role in regulating gene expression often forming complexes with other proteins involved in chromatin remodeling and transcriptional regulation. Its activity helps maintain proper gene expression levels which is critical for cellular functions and development.

Pathways

PHRF1 participates in key biological pathways such as the TGF-beta signaling pathway. It collaborates with SMAD proteins which are important in the TGF-beta signaling cascade influencing cell growth and differentiation. Another important connection is with the Hippo signaling pathway where PHRF1 interacts with proteins involved in controlling organ size and tissue homeostasis.

Mutations or misregulation of PHRF1 have been linked to cancer development particularly breast and lung cancers. Its role in regulating gene expression and cellular growth connects PHRF1 to tumor suppression mechanisms. Additionally it interacts with proteins like HIC1 and CHD8 which modulate tumorigenic pathways and might have implications in cancer progression.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

See full target information PHRF1

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Publications (3)

Recent publications for all applications. Explore the full list and refine your search

Nucleic acids research 53: PubMed40052822

2025

Mono-ubiquitination of TopBP1 by PHRF1 enhances ATR activation and genomic stability.

Applications

Unspecified application

Species

Unspecified reactive species

Fei Zhao,Chenghui Cai,Huanyao Gao,Jaeyoung Moon,Grania Christyani,Sisi Qin,Yalan Hao,Tongzheng Liu,Zhenkun Lou,Wootae Kim

Molecular cell 82:3030-3044.e8 PubMed35764091

2022

An oncogenic JMJD6-DGAT1 axis tunes the epigenetic regulation of lipid droplet formation in clear cell renal cell carcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Jin Zhou,Jeremy M Simon,Chengheng Liao,Cheng Zhang,Lianxin Hu,Giada Zurlo,Xijuan Liu,Cheng Fan,Austin Hepperla,Liwei Jia,Vanina Toffessi Tcheuyap,Hua Zhong,Roy Elias,Jin Ye,W Mike Henne,Payal Kapur,Deepak Nijhawan,James Brugarolas,Qing Zhang

Oncotarget 7:64360-64370 PubMed27608840

2016

Overexpression of PHRF1 attenuates the proliferation and tumorigenicity of non-small cell lung cancer cells.

Applications

Species

Unspecified reactive species

Yadong Wang,Haiyu Wang,Teng Pan,Li Li,Jiangmin Li,Haiyan Yang

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-PHRF1 antibody

Immunoprecipitation - Anti-PHRF1 antibody (AB85974)

Western blot - Anti-PHRF1 antibody (AB85974)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (3)

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Product promise