JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB326610

Anti-PHT1 antibody [EPR26040-123] - BSA and Azide free

Be the first to review this product! Submit a review

|

(0 Publication)

Rabbit Recombinant Monoclonal S15A4 antibody. Carrier free. Suitable for WB and reacts with Human samples.

View Alternative Names

PHT1, PTR4, FP12591, Solute carrier family 15 member 4, Peptide transporter 4, Peptide/histidine transporter 1, hPHT1

4 Images
Western blot - Anti-PHT1 antibody [EPR26040-123] - BSA and Azide free (AB326610)
  • WB

Supplier Data

Western blot - Anti-PHT1 antibody [EPR26040-123] - BSA and Azide free (AB326610)

This data was developed using ab324433, the same antibody clone in a different buffer formulation.

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

The expression profile/ molecular weight observed is consistent with what has been described in the literature (PMID : 33560415; PMID : 37527038).

This blot was developed using a high-sensitivity ECL substrate allowing for the detection of proteins in the mid-femtogram range.

Samples are non-boiled as boiling may cause protein aggregation.

The identity of the lower MW band at approximately 40 kDa is unknown.

In Western blot Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) staining at 1/200000 dilution.

All lanes:

Western blot - Anti-SLC15A4/PHT1 antibody [EPR26040-123] (<a href='/en-us/products/primary-antibodies/slc15a4-pht1-antibody-epr26040-123-ab324433'>ab324433</a>) at 1/1000 dilution

Lane 1:

293T (human embryonic kidney epithelial cell) transfected with scrambled siRNA control whole cell lysate at 40 µg

Lane 2:

293T transfected with siRNA specifically targeting PHT1 whole cell lysate at 40 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Observed band size: 50-100 kDa,36 kDa

true

Exposure time: 103s

Western blot - Anti-PHT1 antibody [EPR26040-123] - BSA and Azide free (AB326610)
  • WB

Supplier Data

Western blot - Anti-PHT1 antibody [EPR26040-123] - BSA and Azide free (AB326610)

This data was developed using ab324433, the same antibody clone in a different buffer formulation.

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

The expression profile/ molecular weight observed is consistent with what has been described in the literature (PMID : 33560415; PMID : 37527038).

Samples are non-boiled as boiling may cause protein aggregation.

PHT1 is a glycoprotein of approximately 50-100 kDa and detected as 37-50 kDa band after treated with Deglycosylation Peptide : N-glycosidase F (PNGase F).

In Western blot Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) staining at 1/200000 dilution.

All lanes:

Western blot - Anti-SLC15A4/PHT1 antibody [EPR26040-123] (<a href='/en-us/products/primary-antibodies/slc15a4-pht1-antibody-epr26040-123-ab324433'>ab324433</a>) at 1/1000 dilution

Lane 1:

Untreated THP-1 (human monocytic leukemia monocyte) whole cell lysate at 40 µg

Lane 2:

THP-1 treated with Peptide:N-glycosidase F (PNGase F) whole cell lysate at 40 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Observed band size: 50-100 kDa,36 kDa

false

Exposure time: 180s

Western blot - Anti-PHT1 antibody [EPR26040-123] - BSA and Azide free (AB326610)
  • WB

Supplier Data

Western blot - Anti-PHT1 antibody [EPR26040-123] - BSA and Azide free (AB326610)

This data was developed using ab324433, the same antibody clone in a different buffer formulation.

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

Low expression : colon (PMID : 11741232 PMID : 11741260)

The expression profile/ molecular weight observed is consistent with what has been described in the literature (PMID : 33560415; PMID : 37527038).

This blot of lanes1-4 and lane 7 was developed using a high-sensitivity ECL substrate allowing for the detection of proteins in the mid-femtogram range.

Samples are non-boiled as boiling may cause protein aggregation.

The identity of the higher MW band at approximately 300 kDa in lane 7 is unknown.

The identity of the lower MW band at approximately 40 kDa in lane 3 is unknown.

Lanes 1-4 and lane 7 are incubated with Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 and lanes 5-6 are incubated with Goat Anti-Rabbit IgG (H+L) Peroxidase conjugated (ab97051) at 1/20000.

In Western blot Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) staining at 1/200000 dilution.

Exposure time : Lanes 1-4 : 48 seconds; Lanes 6-7 : 180 seconds

All lanes:

Western blot - Anti-SLC15A4/PHT1 antibody [EPR26040-123] (<a href='/en-us/products/primary-antibodies/slc15a4-pht1-antibody-epr26040-123-ab324433'>ab324433</a>) at 1/1000 dilution

Lane 1:

Human testis tissue lysate at 48 µg

Lane 2:

Human liver tissue lysate at 48 µg

Lane 3:

Human tonsil tissue lysate at 48 µg

Lane 4:

Human colon tissue lysate at 48 µg

Lane 5:

THP-1 (human monocytic leukemia monocyte) whole cell lysate at 48 µg

Lane 6:

A-673 (human muscle Ewings sarcoma cell line) whole cell lysate at 48 µg

Lane 7:

Human skeletal muscle tissue lysate at 48 µg

Secondary

Lanes 1, 2, 3, 4 and 7:

Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

Lanes 5 - 6:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Observed band size: 50-100 kDa,36 kDa

true

Western blot - Anti-PHT1 antibody [EPR26040-123] - BSA and Azide free (AB326610)
  • WB

Supplier Data

Western blot - Anti-PHT1 antibody [EPR26040-123] - BSA and Azide free (AB326610)

This data was developed using ab324433, the same antibody clone in a different buffer formulation.

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

The expression profile/ molecular weight observed is consistent with what has been described in the literature (PMID : 33560415; PMID : 37527038).

Samples are non-boiled as boiling may cause protein aggregation.

The identity of the lower MW band at approximately 40 kDa in lane 1 and lane 3 is unknown.

In Western blot Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) staining at 1/200000 dilution.

All lanes:

Western blot - Anti-SLC15A4/PHT1 antibody [EPR26040-123] (<a href='/en-us/products/primary-antibodies/slc15a4-pht1-antibody-epr26040-123-ab324433'>ab324433</a>) at 1/1000 dilution

Lane 1:

293T (human embryonic kidney epithelial cell) whole lysate at 40 µg

Lane 2:

293T mitochondrial fraction at 40 µg

Lane 3:

293T non-mitochondrial fraction at 40 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Observed band size: 50-100 kDa,36 kDa,16 kDa

false

Exposure time: 136s

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR26040-123

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Specificity

For this product, the high-sensitive substrate is more recommended.

style
left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>" } } }
style
left-column

Product details

ab326610 is the carrier-free version of ab324433

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

style
left-column

Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
style
left-column
style
left-column
style
left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
style
left-column

Target data

Proton-coupled amino-acid transporter that mediates the transmembrane transport of L-histidine and some di- and tripeptides from inside the lysosome to the cytosol, and plays a key role in innate immune response (PubMed : 16289537, PubMed : 25238095, PubMed : 29224352, PubMed : 39719710). Preferably binds to short peptides with a basic residue at the first position and a hydrophobic residue at the second position (PubMed : 39719710). Able to transport a variety of di- and tripeptides, including carnosine and some peptidoglycans (PubMed : 29224352, PubMed : 31073693). Transporter activity is pH-dependent and maximized in the acidic lysosomal environment (By similarity). Involved in the detection of microbial pathogens by toll-like receptors (TLRs) and NOD-like receptors (NLRs), probably by mediating transport of bacterial peptidoglycans across the endolysosomal membrane : catalyzes the transport of certain bacterial peptidoglycans, such as muramyl dipeptide (MDP), the NOD2 ligand, and L-alanyl-gamma-D-glutamyl-meso-2,6-diaminoheptanedioate (tri-DAP), the NOD1 ligand (PubMed : 25238095, PubMed : 29224352). Required for TLR7, TLR8 and TLR9-mediated type I interferon (IFN-I) productions in plasmacytoid dendritic cells (pDCs) (PubMed : 25238095). Independently of its transporter activity, also promotes the recruitment of innate immune adapter TASL to endolysosome downstream of TLR7, TLR8 and TLR9 : TASL recruitment leads to the specific recruitment and activation of IRF5 (PubMed : 32433612, PubMed : 39719710). Required for isotype class switch recombination to IgG2c isotype in response to TLR9 stimulation (By similarity). Required for mast cell secretory-granule homeostasis by limiting mast cell functions and inflammatory responses (By similarity).
See full target information SLC15A4
style
left-column
style
left-column
style
right-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com