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AB250730

Anti-PHYHD1 antibody [EPR13313(B)] - BSA and Azide free

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(1 Publication)

Rabbit Recombinant Monoclonal PHYHD1 antibody. Carrier free. Suitable for ICC/IF, WB, Flow Cyt (Intra) and reacts with Human samples. Cited in 1 publication.

View Alternative Names

Phytanoyl-CoA dioxygenase domain-containing protein 1, Protein PHYHD1, PHYHD1

3 Images
Flow Cytometry (Intracellular) - Anti-PHYHD1 antibody [EPR13313(B)] - BSA and Azide free (AB250730)
  • Flow Cyt (Intra)

Supplier Data

Flow Cytometry (Intracellular) - Anti-PHYHD1 antibody [EPR13313(B)] - BSA and Azide free (AB250730)

This data was developed using ab184174, the same antibody clone in a different buffer formulation.

Intracellular Flow Cytometry analysis of HepG2 cells fixed in 2% paraformaldehyde labeling PHYHD1 with ab184174 at 1/400 dilution (red). Goat andti rabbit IgG (FITC) was used at 1/150 dilution as secondary antibody. Rabbit monoclonal IgG was used as an isotype control (green).

Immunocytochemistry/ Immunofluorescence - Anti-PHYHD1 antibody [EPR13313(B)] - BSA and Azide free (AB250730)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-PHYHD1 antibody [EPR13313(B)] - BSA and Azide free (AB250730)

This data was developed using ab184174, the same antibody clone in a different buffer formulation.

Immunofluorescent analysis of HepG2 cells fixed in 4% paraformaldehyde labeling PHYHD1 with ab184174 at 1/500 dilution. Goat anti-rabbit IgG(Alexa Fluor® 555) was used at 1/200 dilution for secondary antibody and the cells were counterstained with DAPI.

Western blot - Anti-PHYHD1 antibody [EPR13313(B)] - BSA and Azide free (AB250730)
  • WB

Supplier Data

Western blot - Anti-PHYHD1 antibody [EPR13313(B)] - BSA and Azide free (AB250730)

This data was developed using ab184174, the same antibody clone in a different buffer formulation.

Blocking and dilution buffer : 5% NFDM/TBST.

All lanes:

Western blot - Anti-PHYHD1 antibody [EPR13313(B)] (<a href='/en-us/products/primary-antibodies/phyhd1-antibody-epr13313b-ab184174'>ab184174</a>) at 1/10000 dilution

Lane 1:

Human fetal liver tissue lysate at 20 µg

Lane 2:

Human fetal kidney tissue lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 32 kDa

false

  • Unconjugated

    Anti-PHYHD1 antibody [EPR13313(B)]

  • 660 APC

    APC Anti-PHYHD1 antibody [EPR13313(B)]

  • 519 Alexa Fluor® 488

    Alexa Fluor® 488 Anti-PHYHD1 antibody [EPR13313(B)]

  • 565 Alexa Fluor® 555

    Alexa Fluor® 555 Anti-PHYHD1 antibody [EPR13313(B)]

  • 603 Alexa Fluor® 568

    Alexa Fluor® 568 Anti-PHYHD1 antibody [EPR13313(B)]

  • 617 Alexa Fluor® 594

    Alexa Fluor® 594 Anti-PHYHD1 antibody [EPR13313(B)]

  • 665 Alexa Fluor® 647

    Alexa Fluor® 647 Anti-PHYHD1 antibody [EPR13313(B)]

  • 578 PE

    PE Anti-PHYHD1 antibody [EPR13313(B)]

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR13313(B)

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

ICC/IF, Flow Cyt (Intra), WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "<p></p>" } } }
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Product details

ab250730 is the carrier-free version of ab184174.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The PHYHD1 protein also known as Phytanoyl-CoA Dioxygenase Domain Containing 1 functions as an enzyme involved in metabolic processes. It carries out oxidation reactions specifically hydroxylation on specific substrate molecules. PHYHD1 has a molecular mass of approximately 44 kDa making it a moderate-sized protein. Expressed in various tissues including the liver and kidneys PHYHD1 plays a significant role in managing metabolic activity within these regions contributing to cellular homeostasis and overall metabolic health.
Biological function summary

PHYHD1 participates in cellular metabolism by catalyzing reactions that modify fatty acids. This protein is involved in the breakdown of phytanic acid a complex fatty acid. In these reactions PHYHD1 may function independently or as part of larger protein complexes facilitating intricate steps that convert fatty acids into more usable forms. This process represents a significant part of lipid metabolism illustrating PHYHD1's role in maintaining the balance of lipid levels and energy within the cell.

Pathways

Researchers identified PHYHD1 in pathways related to fatty acid metabolism and chain elongation. In the context of the peroxisomal alpha-oxidation pathway PHYHD1 interacts with important proteins such as Peroxin and Phytanoyl-CoA alpha-hydroxylase which together assist in processing certain fatty acids. Additionally PHYHD1 links to other metabolic pathways involving oxidation reactions highlighting its central role in modulating lipid and energy metabolism.

Studies associate PHYHD1 with conditions such as Refsum Disease and disorders of fatty acid metabolism. Refsum Disease a genetic disorder involving the accumulation of phytanic acid sees a loss or reduction in PHYHD1 activity leading to severe symptoms. Through these associations PHYHD1 interacts with proteins like the phytanoyl-CoA hydroxylase enzyme exemplifying its critical role in the breakdown of fatty acids and linking its activity directly to disease state development and progression.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

2-oxoglutarate(2OG)-dependent dioxygenase that catalyzes the conversion of 2-oxoglutarate to succinate and CO(2) in an iron-dependent manner (PubMed : 21530488). However, does not couple 2OG turnover to the hydroxylation of acyl-coenzyme A derivatives, implying that it is not directly involved in phytanoyl coenzyme-A metabolism (PubMed : 21530488). Does not show detectable activity towards fatty acid CoA thioesters (PubMed : 21530488).. Isoform 2. Isoform 2 probably lacks enzyme activity.. Isoform 3. Isoform 3 probably lacks enzyme activity.
See full target information PHYHD1
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Nature cell biology 24:1541-1557 PubMed36192632

2022

Alternative RNA splicing modulates ribosomal composition and determines the spatial phenotype of glioblastoma cells.

Applications

Unspecified application

Species

Unspecified reactive species

Tatyana D Larionova,Soniya Bastola,Tatiana E Aksinina,Ksenia S Anufrieva,Jia Wang,Victoria O Shender,Dmitriy E Andreev,Tatiana F Kovalenko,Georgij P Arapidi,Polina V Shnaider,Anastasia N Kazakova,Yaroslav A Latyshev,Victor V Tatarskiy,Alexander A Shtil,Pascale Moreau,Francis Giraud,Chaoxi Li,Yichan Wang,Maria P Rubtsova,Olga A Dontsova,Michael Condro,Benjamin M Ellingson,Mikhail I Shakhparonov,Harley I Kornblum,Ichiro Nakano,Marat S Pavlyukov
View all publications
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com