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AB133172

Anti-PI-15 antibody

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(1 Publication)

Goat Polyclonal PI-15 antibody. Suitable for IHC-P, WB and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Synthetic Peptide within Human PI15 aa 100-150.

View Alternative Names

CRISP8, P25TI, PI15, Peptidase inhibitor 15, PI-15, 25 kDa trypsin inhibitor, Cysteine-rich secretory protein 8, SugarCrisp, p25TI, CRISP-8

1 Images
Western blot - Anti-PI-15 antibody (AB133172)
  • WB

Unknown

Western blot - Anti-PI-15 antibody (AB133172)

All lanes:

Western blot - Anti-PI-15 antibody (ab133172) at 0.3 µg/mL

All lanes:

HeLa cell lysate in RIPA buffer at 35 µg

Predicted band size: 29 kDa

Observed band size: 33 kDa

true

Key facts

Host species

Goat

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

IHC-P, WB

applications

Immunogen

Synthetic Peptide within Human PI15 aa 100-150. The exact immunogen used to generate this antibody is proprietary information.

O43692

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "5 µg/mL", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.3-1 µg/mL", "WB-species-notes": "<p>1 hour primary incubation is recommended for this product.</p>" }, "Mouse": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rat": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Chicken": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Cow": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Dog": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Pig": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rabbit": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Zebrafish": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Purification notes
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Storage buffer
pH: 7.3 Preservative: 0.02% Sodium azide Constituents: 99% Tris buffered saline, 0.5% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

PI-15 also known as Peptidase Inhibitor 15 is a secreted protein characterized by a mass of approximately 12 kDa. This inhibitor is predominantly expressed in the nervous system especially in the human brain and spinal cord. Its primary role involves the regulation of protease activity. The PI-15 protein inhibits trypsin-like serine proteases modulating proteolytic processes that are critical for cellular function.
Biological function summary

PI-15 participates actively in the modulation of protease activity to maintain cellular homeostasis. It does not form parts of larger complexes but exerts its effects individually by inhibiting serine proteases. This inhibition impacts a range of biological processes including cellular differentiation and tissue remodeling. The inhibitory function serves as a regulatory step in proteolytic cascades protecting cells from excessive protease activity that can be damaging.

Pathways

PI-15 integrates into the regulatory landscape of the protease inhibition pathway. Through this pathway it interacts indirectly with proteins such as plasminogen activators contributing to the control of fibrinolysis and matrix degradation. Another pathway involving PI-15 is the extracellular matrix (ECM) remodeling pathway where it helps in managing ECM turnover by inhibiting serine proteases that degrade matrix components maintaining structural integrity.

PI-15 has relevance in neurological conditions and cancer. Its role in the nervous system links it with neurodegenerative diseases possibly affecting the disease process by altering proteostasis. In cancer altered PI-15 expression may influence tumor progression through its effect on ECM remodeling and cellular invasion. The serine proteases targeted by PI-15 including urokinase-type plasminogen activator have also established connections to cancer metastasis emphasizing the importance of PI-15 in cancer biology.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Serine protease inhibitor which displays weak inhibitory activity against trypsin (PubMed : 8882727). May play a role in facial patterning during embryonic development (By similarity).
See full target information PI15
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Scientific reports 9:15244 PubMed31645712

2019

Epithelial EP4 plays an essential role in maintaining homeostasis in colon.

Applications

Unspecified application

Species

Unspecified reactive species

Yoshihide Matsumoto,Yuki Nakanishi,Takuto Yoshioka,Yuichi Yamaga,Tomonori Masuda,Yuichi Fukunaga,Makoto Sono,Takaaki Yoshikawa,Munemasa Nagao,Osamu Araki,Satoshi Ogawa,Norihiro Goto,Yukiko Hiramatsu,Richard M Breyer,Akihisa Fukuda,Hiroshi Seno
View all publications
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Product promise

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