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Rabbit Recombinant Monoclonal PI 3 Kinase catalytic subunit alpha/PIK3CA antibody. Suitable for IP, WB and reacts with Mouse, Rat, Human samples. Cited in 3 publications.

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Images

Immunoprecipitation - Anti-PI 3 Kinase catalytic subunit alpha/PIK3CA antibody [EPR19693] (AB183957), expandable thumbnail
  • Western blot - Anti-PI 3 Kinase catalytic subunit alpha/PIK3CA antibody [EPR19693] (AB183957), expandable thumbnail
  • Western blot - Anti-PI 3 Kinase catalytic subunit alpha/PIK3CA antibody [EPR19693] (AB183957), expandable thumbnail
  • Western blot - Anti-PI 3 Kinase catalytic subunit alpha/PIK3CA antibody [EPR19693] (AB183957), expandable thumbnail

Publications

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IPWB
Human
Expected
Tested
Mouse
Tested
Tested
Rat
Expected
Tested

Tested
Tested

Species
Mouse
Dilution info
1/30
Notes

-

Expected
Expected

Species
Rat, Human
Dilution info
Use at an assay dependent concentration.
Notes

-

Tested
Tested

Species
Mouse
Dilution info
1/1000
Notes

-

Species
Rat
Dilution info
1/1000
Notes

-

Species
Human
Dilution info
1/1000
Notes

-

Associated Products

Select an associated product type

6 products for Alternative Product

Target data

Function

Phosphoinositide-3-kinase (PI3K) phosphorylates phosphatidylinositol (PI) and its phosphorylated derivatives at position 3 of the inositol ring to produce 3-phosphoinositides (PubMed:15135396, PubMed:23936502, PubMed:28676499). Uses ATP and PtdIns(4,5)P2 (phosphatidylinositol 4,5-bisphosphate) to generate phosphatidylinositol 3,4,5-trisphosphate (PIP3) (PubMed:15135396, PubMed:28676499). PIP3 plays a key role by recruiting PH domain-containing proteins to the membrane, including AKT1 and PDPK1, activating signaling cascades involved in cell growth, survival, proliferation, motility and morphology. Participates in cellular signaling in response to various growth factors. Involved in the activation of AKT1 upon stimulation by receptor tyrosine kinases ligands such as EGF, insulin, IGF1, VEGFA and PDGF. Involved in signaling via insulin-receptor substrate (IRS) proteins. Essential in endothelial cell migration during vascular development through VEGFA signaling, possibly by regulating RhoA activity. Required for lymphatic vasculature development, possibly by binding to RAS and by activation by EGF and FGF2, but not by PDGF. Regulates invadopodia formation through the PDPK1-AKT1 pathway. Participates in cardiomyogenesis in embryonic stem cells through a AKT1 pathway. Participates in vasculogenesis in embryonic stem cells through PDK1 and protein kinase C pathway. In addition to its lipid kinase activity, it displays a serine-protein kinase activity that results in the autophosphorylation of the p85alpha regulatory subunit as well as phosphorylation of other proteins such as 4EBP1, H-Ras, the IL-3 beta c receptor and possibly others (PubMed:23936502, PubMed:28676499). Plays a role in the positive regulation of phagocytosis and pinocytosis (By similarity).

Alternative names

PI3-kinase subunit alpha, PI3K-alpha, PI3Kalpha, PtdIns-3-kinase subunit alpha, Phosphoinositide 3-kinase alpha, Phosphoinositide-3-kinase catalytic alpha polypeptide, Serine/threonine protein kinase PIK3CA, PtdIns-3-kinase subunit p110-alpha, p110alpha, PIK3CA

Recommended products

Rabbit Recombinant Monoclonal PI 3 Kinase catalytic subunit alpha/PIK3CA antibody. Suitable for IP, WB and reacts with Mouse, Rat, Human samples. Cited in 3 publications.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number
EPR19693
Purification technique
Affinity purification Protein A
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle

Notes

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

PI 3 kinase catalytic subunit alpha also known as PIK3CA or PI3K-alpha is a protein kinase with significant roles in cellular signaling. This protein has a mass of approximately 124 kDa and is part of the PI3 kinase family. PIK3CA is primarily expressed in various tissues including the liver breast and pancreas where it plays an important role in the regulation of cell growth and survival. The structure of PI3 kinase involves a complex of p110 catalytic subunits and p85 regulatory subunits which facilitate its enzymatic functions.

Biological function summary

The PIK3CA protein phosphorylates the inositol ring of phosphoinositides generating phosphatidylinositol (345)-trisphosphate (PIP3) a lipid second messenger involved in numerous signaling pathways. PIK3CA often acts within a complex interacting with regulatory subunits such as p85 to control its activity. The complex formation enhances its stability and localization to the cell membrane where it can exert its enzymatic actions effectively. In its biological capacity PIK3CA plays a role in cellular proliferation survival and metabolism.

Pathways

The PI3K/Akt signaling pathway involves PIK3CA as an important mediator. This pathway is integral to numerous cellular processes including metabolism growth and survival. PIK3CA links closely with Akt a downstream protein kinase that propagates the signal initiated by PIP3 generation. The mTOR pathway is another critical route involving PIK3CA where it regulates translation and cellular growth. These pathways emphasize the importance of PIK3CA in maintaining cellular homeostasis and responding to external growth signals.

Associated diseases and disorders

Mutations and aberrations in PIK3CA associate with several cancers especially breast and colorectal cancers. The altered activity of PIK3CA can cause uncontrolled cell proliferation due to constant activation of the PI3K/Akt signaling pathway. This disrupts normal cellular apoptosis and fosters tumor growth. Additionally PIK3CA mutations are linked to certain genetic disorders such as PIK3CA-related overgrowth spectrum (PROS) where overactivation alters normal tissue growth and development. The interaction of PIK3CA with proteins like PTEN a tumor suppressor involved in cancer pathways also highlights its significance in oncogenic processes.

Product promise

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Full details and terms and conditions can be found here:
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4 product images

  • Immunoprecipitation - Anti-PI 3 Kinase catalytic subunit alpha/PIK3CA antibody [EPR19693] (ab183957), expandable thumbnail

    Immunoprecipitation - Anti-PI 3 Kinase catalytic subunit alpha/PIK3CA antibody [EPR19693] (ab183957)

    PI 3 Kinase catalytic subunit alpha/PIK3CA was immunoprecipitated from 0.35 mg of mouse hypothalamus lysate with ab183957 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab183957 at 1/500 dilution. VeriBlot for IP Detection Reagent (HRP) (VeriBlot for IP Detection Reagent (HRP) ab131366), was used for detection at 1/1000 dilution.

    Lane 1: Mouse hypothalamus lysate, 10μg (Input).
    Lane 2: ab183957 IP in mouse hypothalamus lysate.
    Lane 3: Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of ab183957 in mouse hypothalamus lysate.

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.
    Exposure time: 3 minutes.

    All lanes: Immunoprecipitation - Anti-PI 3 Kinase catalytic subunit alpha/PIK3CA antibody [EPR19693] (ab183957)

    Predicted band size: 124 kDa

    Observed band size: 110 kDa

  • Western blot - Anti-PI 3 Kinase catalytic subunit alpha/PIK3CA antibody [EPR19693] (ab183957), expandable thumbnail

    Western blot - Anti-PI 3 Kinase catalytic subunit alpha/PIK3CA antibody [EPR19693] (ab183957)

    Lane 1: Wild type HAP1 whole cell lysate (20 μg)
    Lane 2: PI 3 Kinase catalytic subunit alpha/PIK3CA knockout HAP1 whole cell lysate (20 μg)
    Lane 3: HeLa whole cell lysate (20 μg)
    Lane 4: Jurkat whole cell lysate (20 μg)

    Lanes 1 - 4: Merged signal (red and green). Green - ab183957 observed at 125 kDa. Red - loading control, Anti-GAPDH antibody [mAbcam 9484] - Loading Control ab9484, observed at 37 kDa.

    ab183957 was shown to recognize PI 3 Kinase catalytic subunit alpha/PIK3CA in wild type cells as signal was lost at the expected MW in PI 3 Kinase catalytic subunit alpha/PIK3CA knockout cells. Additional cross-reactive bands were observed in the wild-type and knockout cells. Wild-type and PI 3 Kinase catalytic subunit alpha/PIK3CA knockout samples were subjected to SDS-PAGE. ab183957 and Anti-GAPDH antibody [mAbcam 9484] - Loading Control ab9484 (Mouse anti-GAPDH loading control) were incubated overnight at 4°C at 1/1000 dilution and 1/20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776 secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.

    All lanes: Western blot - Anti-PI 3 Kinase catalytic subunit alpha/PIK3CA antibody [EPR19693] (ab183957)

    Predicted band size: 124 kDa

  • Western blot - Anti-PI 3 Kinase catalytic subunit alpha/PIK3CA antibody [EPR19693] (ab183957), expandable thumbnail

    Western blot - Anti-PI 3 Kinase catalytic subunit alpha/PIK3CA antibody [EPR19693] (ab183957)

    Blocking/Dilution buffer: 5% NFDM/TBST.

    Exposure time: Lane 1: 3 minutes; Lane 2 and 3: 5 seconds; Lane 4: 3 seconds.

    The observed molecular weight is consistent with what has been described in the literature (PMID: 19701705).

    All lanes: Western blot - Anti-PI 3 Kinase catalytic subunit alpha/PIK3CA antibody [EPR19693] (ab183957) at 1/1000 dilution

    Lane 1: F9 (Mouse embryonic testicular cancer cell line) whole cell lysate at 20 µg

    Lane 2: M1 (Mouse myeloblast myeloid leukemia cell line) whole cell lysate at 20 µg

    Lane 3: Mouse hypothalamus lysate at 20 µg

    Lane 4: Mouse fetal brain lysate at 20 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution

    Predicted band size: 124 kDa

    Observed band size: 110 kDa

  • Western blot - Anti-PI 3 Kinase catalytic subunit alpha/PIK3CA antibody [EPR19693] (ab183957), expandable thumbnail

    Western blot - Anti-PI 3 Kinase catalytic subunit alpha/PIK3CA antibody [EPR19693] (ab183957)

    Blocking/Dilution buffer: 5% NFDM/TBST.

    The observed molecular weight is consistent with what has been described in the literature (PMID: 19701705).

    All lanes: Western blot - Anti-PI 3 Kinase catalytic subunit alpha/PIK3CA antibody [EPR19693] (ab183957) at 1/1000 dilution

    All lanes: P0 Rat brain lysate at 20 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution

    Predicted band size: 124 kDa

    Observed band size: 110 kDa

    Exposure time: 1min

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Product protocols

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