Anti-PI 3 Kinase catalytic subunit alpha/PIK3CA antibody [SP139]

• Flow Cyt (Intra)

Unknown

Flow Cytometry (Intracellular) - Anti-PI 3 Kinase catalytic subunit alpha/PIK3CA antibody [SP139] (AB135384)

Flow Cytometry analysis of HeLa (human cervix adenocarcinoma epithelial cell) cells labeling PI 3 Kinase catalytic subunit alpha/PIK3CA with purified ab135384 at 1 : 250 dilution (1.00 µg/ml) Red. Cells were fixed with 4% paraformaldehyde . A Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) secondary antibody was used at 1 : 2000 dilution. Isotype control - Rabbit monoclonal IgG (ab172730) / Black. Unlabeled control - Unlabelled cells / Blue.

• IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PI 3 Kinase catalytic subunit alpha/PIK3CA antibody [SP139] (AB135384)

Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human breast carcinoma tissue labelling PI 3 Kinase catalytic subunit alpha/PIK3CA with ab135384 at 1/100 dilution.

• ICC

Lab

Immunocytochemistry - Anti-PI 3 Kinase catalytic subunit alpha/PIK3CA antibody [SP139] (AB135384)

Immunocytochemistry/ Immunofluorescence analysis of HeLa (human cervix adenocarcinoma epithelial cell) cells labeling PI 3 Kinase catalytic subunit alpha/PIK3CA with purified ab135384 at 1/25 dilution (10 μg/mL). Cells were fixed in 4% paraformaldehyde and permeabilized with 0.1% Triton X-100. Cells were counterstained with ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor^® 594) 1/200 dilution (2.5 μg/mL). Goat anti rabbit IgG (Alexa Fluor^® 488, ab150077) was used as the secondary antibody at 1/1000 dilution (2 μg/mL). DAPI nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control. Confocal image showing cytoplasmic staining in HeLa cells.

• Flow Cyt (Intra)

Unknown

Flow Cytometry (Intracellular) - Anti-PI 3 Kinase catalytic subunit alpha/PIK3CA antibody [SP139] (AB135384)

Flow Cytometry analysis of C6(Rat glial tumor glial cell) cells labeling PI 3 Kinase catalytic subunit alpha/PIK3CA with purified ab135384 at 1 : 250 dilution (1.00 µg/ml) Red. Cells were fixed with 4% paraformaldehyde . A Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) secondary antibody was used at 1 : 2000 dilution. Isotype control - Rabbit monoclonal IgG (ab172730) / Black. Unlabeled control - Unlabelled cells / Blue.

• ICC/IF

Lab

Immunocytochemistry/ Immunofluorescence - Anti-PI 3 Kinase catalytic subunit alpha/PIK3CA antibody [SP139] (AB135384)

Immunocytochemistry/ Immunofluorescence analysis of C6 (rat glial tumor glial cell) cells labeling PI 3 Kinase catalytic subunit alpha/PIK3CA with purified ab135384 at 1 : 25(10 µg/ml). Cells were fixed in 4% paraformaldehyde and permeabilized with 0.1% Triton X-100. Cells were counterstained with ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1 : 200 (2.5 µg/ml). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) was used as the secondary antibody at 1 : 1000 (2 µg/ml) dilution. DAPI nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.

• IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PI 3 Kinase catalytic subunit alpha/PIK3CA antibody [SP139] (AB135384)

Immunohistochemical analysis of paraffin-embedded Rat stomach tissue labelling PI 3 Kinase catalytic subunit alpha/PIK3CA with ab135384 at 1/100 dilution, followed by Goat Anti-Rabbit & Mouse IgG (HRP). Mainly membranous staining on the rat stomach, performed on a Leica Biosystems BOND® RX instrument.

The section was incubated with ab135384 for 10 mins at room temperature. Counter stained with Hematoxylin. Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit & Mouse IgG (HRP).

• IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PI 3 Kinase catalytic subunit alpha/PIK3CA antibody [SP139] (AB135384)

Immunohistochemical analysis of paraffin-embedded Human breast carcinoma tissue labelling PI 3 Kinase catalytic subunit alpha/PIK3CA with ab135384 at 1/100 dilution, followed by Goat Anti-Rabbit & Mouse IgG (HRP). Mainly membranous staining on the human breast carcinoma, performed on a Leica Biosystems BOND® RX instrument.

The section was incubated with ab135384 for 10 mins at room temperature. Counter stained with Hematoxylin. Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit & Mouse IgG (HRP).