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Mouse Monoclonal PI 3 Kinase catalytic subunit gamma/PI3K-gamma antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Human, African green monkey samples. Cited in 42 publications. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human PIK3CG.

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Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PI 3 Kinase catalytic subunit gamma/PI3K-gamma antibody [OTI4G10] (AB140307), expandable thumbnail
  • Western blot - Anti-PI 3 Kinase catalytic subunit gamma/PI3K-gamma antibody [OTI4G10] (AB140307), expandable thumbnail
  • Immunocytochemistry/ Immunofluorescence - Anti-PI 3 Kinase catalytic subunit gamma/PI3K-gamma antibody [OTI4G10] (AB140307), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PI 3 Kinase catalytic subunit gamma/PI3K-gamma antibody [OTI4G10] (AB140307), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PI 3 Kinase catalytic subunit gamma/PI3K-gamma antibody [OTI4G10] (AB140307), expandable thumbnail

Publications

Key facts

Isotype
IgG2a
Host species
Mouse
Storage buffer

pH: 7.3
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol (glycerin, glycerine), 1% BSA

Form
Liquid
Clonality
Monoclonal

Immunogen

  • Recombinant Full Length Protein corresponding to Human PIK3CG. Database link P48736

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IHC-PWBICC/IF
Human
Tested
Tested
Expected
Mouse
Predicted
Predicted
Predicted
African green monkey
Expected
Expected
Tested
Pig
Predicted
Predicted
Predicted

Tested
Tested

Species
Human
Dilution info
1/150
Notes

Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Expected
Expected

Species
African green monkey
Dilution info
Use at an assay dependent concentration.
Notes

-

Predicted
Predicted

Species
Mouse, Pig
Dilution info
-
Notes

-

Tested
Tested

Species
Human
Dilution info
1/2000
Notes

-

Expected
Expected

Species
African green monkey
Dilution info
Use at an assay dependent concentration.
Notes

-

Predicted
Predicted

Species
Mouse, Pig
Dilution info
-
Notes

-

Tested
Tested

Species
African green monkey
Dilution info
1/100
Notes

-

Expected
Expected

Species
Human
Dilution info
Use at an assay dependent concentration.
Notes

-

Predicted
Predicted

Species
Mouse, Pig
Dilution info
-
Notes

-

Associated Products

Select an associated product type

2 products for Alternative Product

Target data

Function

Phosphoinositide-3-kinase (PI3K) that phosphorylates PtdIns(4,5)P2 (Phosphatidylinositol 4,5-bisphosphate) to generate phosphatidylinositol 3,4,5-trisphosphate (PIP3). PIP3 plays a key role by recruiting PH domain-containing proteins to the membrane, including AKT1 and PDPK1, activating signaling cascades involved in cell growth, survival, proliferation, motility and morphology. Links G-protein coupled receptor activation to PIP3 production. Involved in immune, inflammatory and allergic responses. Modulates leukocyte chemotaxis to inflammatory sites and in response to chemoattractant agents. May control leukocyte polarization and migration by regulating the spatial accumulation of PIP3 and by regulating the organization of F-actin formation and integrin-based adhesion at the leading edge. Controls motility of dendritic cells. Together with PIK3CD is involved in natural killer (NK) cell development and migration towards the sites of inflammation. Participates in T-lymphocyte migration. Regulates T-lymphocyte proliferation, activation, and cytokine production. Together with PIK3CD participates in T-lymphocyte development. Required for B-lymphocyte development and signaling. Together with PIK3CD participates in neutrophil respiratory burst. Together with PIK3CD is involved in neutrophil chemotaxis and extravasation. Together with PIK3CB promotes platelet aggregation and thrombosis. Regulates alpha-IIb/beta-3 integrins (ITGA2B/ ITGB3) adhesive function in platelets downstream of P2Y12 through a lipid kinase activity-independent mechanism. May have also a lipid kinase activity-dependent function in platelet aggregation. Involved in endothelial progenitor cell migration. Negative regulator of cardiac contractility. Modulates cardiac contractility by anchoring protein kinase A (PKA) and PDE3B activation, reducing cAMP levels. Regulates cardiac contractility also by promoting beta-adrenergic receptor internalization by binding to GRK2 and by non-muscle tropomyosin phosphorylation. Also has serine/threonine protein kinase activity: both lipid and protein kinase activities are required for beta-adrenergic receptor endocytosis. May also have a scaffolding role in modulating cardiac contractility. Contributes to cardiac hypertrophy under pathological stress. Through simultaneous binding of PDE3B to RAPGEF3 and PIK3R6 is assembled in a signaling complex in which the PI3K gamma complex is activated by RAPGEF3 and which is involved in angiogenesis. In neutrophils, participates in a phospholipase C-activating N-formyl peptide-activated GPCR (G protein-coupled receptor) signaling pathway downstream of RASGRP4-mediated Ras-activation, to promote neutrophil functional responses (By similarity).

Alternative names

PI3-kinase subunit gamma, PI3K-gamma, PI3Kgamma, PtdIns-3-kinase subunit gamma, Phosphoinositide-3-kinase catalytic gamma polypeptide, Serine/threonine protein kinase PIK3CG, p120-PI3K, PtdIns-3-kinase subunit p110-gamma, p110gamma, PIK3CG

Recommended products

Mouse Monoclonal PI 3 Kinase catalytic subunit gamma/PI3K-gamma antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Human, African green monkey samples. Cited in 42 publications. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human PIK3CG.

Key facts

Isotype
IgG2a
Form
Liquid
Clonality
Monoclonal
Immunogen
  • Recombinant Full Length Protein corresponding to Human PIK3CG. Database link P48736
Clone number
OTI4G10
Purification technique
Affinity purification
Concentration
Loading...
Purification notes

Purified from cell culture supernatant.

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle

Notes

Clone OTI4G10 (formerly 4G10).

Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.

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Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

PI 3 Kinase catalytic subunit gamma also known as PI3K-gamma is an enzyme important in cellular signaling. PI3K-gamma is part of the PI3K family which includes different classes of signal transducing enzymes. The catalytic subunit with a molecular weight of approximately 110 kDa becomes activated primarily in response to activation of G protein-coupled receptors. It is abundantly expressed in hematopoietic cells but also found in other tissues like the heart and lungs indicating its significant role in diverse cellular contexts. The enzyme functions by phosphorylating phosphoinositides an important step in initiating various downstream signaling pathways.

Biological function summary

PI3K-gamma plays an important role in mediating immune responses. It is a part of a larger dimeric complex coupling with a regulatory subunit to modulate its activity and specificity. This complex formation is vital in regulating leukocyte chemotaxis endothelial cell migration and cardiac function. Signaling through PI3K-gamma influences the activation and migration of immune cells aiding in the body's defense mechanism against infections and inflammation. Additionally the protein regulates heart contractility contributing to cardiac physiology.

Pathways

PI3K-gamma is an essential component in the PI3K-Akt signaling pathway. This pathway modulates various cellular processes including cell proliferation survival and metabolism. Reports link PI3K-gamma to the regulation of mTOR pathway which further affects protein synthesis and autophagy. Within these pathways it aligns with proteins such as Akt and mTORC1 facilitating signals that control cell growth and resistance to apoptosis. These pathways demonstrate the enzyme's involvement in maintenance of cellular homeostasis and adaptability.

Associated diseases and disorders

PI3K-gamma has been implicated in inflammatory diseases and cardiovascular disorders. Enhanced PI3K-gamma activity often correlates with hyperactive immune responses contributing to conditions like rheumatoid arthritis and asthma. Its aberrant signaling also associates with heart failure due to its regulatory role in cardiac myocyte function. In the context of these diseases there is interaction with proteins such as NF-kB and Bcl-2 which get involved in pro-inflammatory signaling and cell survival respectively. The regulation of PI3K-gamma offers potential therapeutic targets for managing these conditions effectively.

Product promise

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5 product images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PI 3 Kinase catalytic subunit gamma/PI3K-gamma antibody [OTI4G10] (ab140307), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PI 3 Kinase catalytic subunit gamma/PI3K-gamma antibody [OTI4G10] (ab140307)

    Paraffin-embedded human ovary adenocarcinoma tissue stained for PI 3 Kinase catalytic subunit gamma/PI3K-gamma using ab140307 at 1/150 dilution in immunohistochemical analysis.

  • Western blot - Anti-PI 3 Kinase catalytic subunit gamma/PI3K-gamma antibody [OTI4G10] (ab140307), expandable thumbnail

    Western blot - Anti-PI 3 Kinase catalytic subunit gamma/PI3K-gamma antibody [OTI4G10] (ab140307)

    All lanes: Western blot - Anti-PI 3 Kinase catalytic subunit gamma/PI3K-gamma antibody [OTI4G10] (ab140307) at 1/2000 dilution

    Lane 1: HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) cell lysate transfected with pCMV6-ENTRY control cDNA at 5 µg

    Lane 2: HEK-293T cell lysate transfected with pCMV6-ENTRY PI 3 Kinase catalytic subunit gamma/PI3K-gamma cDNA at 5 µg

    Predicted band size: 126 kDa

  • Immunocytochemistry/ Immunofluorescence - Anti-PI 3 Kinase catalytic subunit gamma/PI3K-gamma antibody [OTI4G10] (ab140307), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-PI 3 Kinase catalytic subunit gamma/PI3K-gamma antibody [OTI4G10] (ab140307)

    COS-7 (african green monkey kidney fibroblast-like cell line) cells transiently transfected by pCMV6-ENTRY PI 3 Kinase catalytic subunit gamma/PI3K-gamma cDNA, labelling PI 3 Kinase catalytic subunit gamma/PI3K-gamma (green) with ab140307 at 1/100 dilution in ICC/IF.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PI 3 Kinase catalytic subunit gamma/PI3K-gamma antibody [OTI4G10] (ab140307), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PI 3 Kinase catalytic subunit gamma/PI3K-gamma antibody [OTI4G10] (ab140307)

    PI 3 Kinase catalytic subunit gamma/PI3K-gamma Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) staining of Human liver tissue using mouse Anti-PI 3 Kinase catalytic subunit gamma/PI3K-gamma antibody

    Negative control image: IHC image of PI3K gamma staining in a section of formalin-fixed paraffin-embedded normal human liver* performed on a Leica Biosystems BOND® RX instrument using the standard protocol. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20mins. The section was then incubated with ab140307, 10ug/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. The inset secondary-only control image is taken from an identical assay without primary antibody.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

    *Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PI 3 Kinase catalytic subunit gamma/PI3K-gamma antibody [OTI4G10] (ab140307), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PI 3 Kinase catalytic subunit gamma/PI3K-gamma antibody [OTI4G10] (ab140307)

    PI 3 Kinase catalytic subunit gamma/PI3K-gamma Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) staining of Human pancreas tissue using mouse Anti-PI 3 Kinase catalytic subunit gamma/PI3K-gamma antibody

    IHC image of PI3K gamma staining in a section of formalin-fixed paraffin-embedded normal human pancreas* performed on a Leica Biosystems BOND® RX instrument using the standard protocol. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20mins. The section was then incubated with ab140307, 10ug/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. The inset secondary-only control image is taken from an identical assay without primary antibody.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

    *Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre

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