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Rabbit Recombinant Monoclonal PI 3 Kinase Class 2A/Cpk antibody. Carrier free. Suitable for WB and reacts with Human, Mouse, Rat samples. Immunogen corresponding to Synthetic Peptide within Human PIK3C2A conjugated to Keyhole Limpet Haemocyanin.

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Images

Western blot - Anti-PI 3 Kinase Class 2A/Cpk antibody [HL1612] - BSA and Azide free (AB313861), expandable thumbnail
  • Western blot - Anti-PI 3 Kinase Class 2A/Cpk antibody [HL1612] - BSA and Azide free (AB313861), expandable thumbnail
  • Western blot - Anti-PI 3 Kinase Class 2A/Cpk antibody [HL1612] - BSA and Azide free (AB313861), expandable thumbnail

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7.4
Constituents: 100% PBS

Form
Liquid
Clonality
Monoclonal

Immunogen

  • Synthetic Peptide within Human PIK3C2A conjugated to Keyhole Limpet Haemocyanin. Database link O00443

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
WB
Human
Tested
Mouse
Tested
Rat
Tested

Tested
Tested

Species
Human
Dilution info
1/500.00000 - 1/3000.00000
Notes

-

Species
Mouse
Dilution info
1/500.00000 - 1/3000.00000
Notes

-

Species
Rat
Dilution info
1/500.00000 - 1/3000.00000
Notes

-

Target data

Function

Generates phosphatidylinositol 3-phosphate (PtdIns3P) and phosphatidylinositol 3,4-bisphosphate (PtdIns(3,4)P2) that act as second messengers. Has a role in several intracellular trafficking events. Functions in insulin signaling and secretion. Required for translocation of the glucose transporter SLC2A4/GLUT4 to the plasma membrane and glucose uptake in response to insulin-mediated RHOQ activation. Regulates insulin secretion through two different mechanisms: involved in glucose-induced insulin secretion downstream of insulin receptor in a pathway that involves AKT1 activation and TBC1D4/AS160 phosphorylation, and participates in the late step of insulin granule exocytosis probably in insulin granule fusion. Synthesizes PtdIns3P in response to insulin signaling. Functions in clathrin-coated endocytic vesicle formation and distribution. Regulates dynamin-independent endocytosis, probably by recruiting EEA1 to internalizing vesicles. In neurosecretory cells synthesizes PtdIns3P on large dense core vesicles. Participates in calcium induced contraction of vascular smooth muscle by regulating myosin light chain (MLC) phosphorylation through a mechanism involving Rho kinase-dependent phosphorylation of the MLCP-regulatory subunit MYPT1. May play a role in the EGF signaling cascade. May be involved in mitosis and UV-induced damage response. Required for maintenance of normal renal structure and function by supporting normal podocyte function. Involved in the regulation of ciliogenesis and trafficking of ciliary components (PubMed:31034465).

Additional Targets

Pik3c2a

Alternative names

Phosphatidylinositol 4-phosphate 3-kinase C2 domain-containing subunit alpha, PI3K-C2-alpha, PtdIns-3-kinase C2 subunit alpha, Phosphoinositide 3-kinase-C2-alpha, PIK3C2A

Recommended products

Rabbit Recombinant Monoclonal PI 3 Kinase Class 2A/Cpk antibody. Carrier free. Suitable for WB and reacts with Human, Mouse, Rat samples. Immunogen corresponding to Synthetic Peptide within Human PIK3C2A conjugated to Keyhole Limpet Haemocyanin.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • Synthetic Peptide within Human PIK3C2A conjugated to Keyhole Limpet Haemocyanin. Database link O00443
Carrier free
Yes
Clone number
HL1612
Purification technique
Affinity purification Protein A
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Aliquoting information
Upon delivery aliquot

Notes

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

PI 3 Kinase Class 2A also known as Cpk is a significant enzyme involved in the regulation of various cellular processes. The target weighing approximately 170 kDa acts as a lipid kinase that phosphorylates phosphatidylinositol converting PI to PI3P. Cpk expresses mainly in tissues like the liver muscle and heart where it contributes to cellular homeostasis and metabolic regulation.

Biological function summary

This enzyme family modulates intracellular signaling cascades influencing processes such as cell growth survival and metabolism. Cpk forms part of a larger complex that interacts with adaptor proteins facilitating its recruitment to cellular membranes. This interaction is important for its signaling activity and function in maintaining cellular equilibrium.

Pathways

PI 3 Kinase Class 2A plays an integral role in the insulin signaling pathway and the TOR signaling pathway. In the insulin pathway it collaborates with proteins like Akt to modulate glucose uptake and storage in cells. Similarly in the TOR pathway Cpk contributes to nutrient sensing and cell growth regulation interconnected with proteins such as mTOR affecting protein synthesis and autophagy.

Associated diseases and disorders

Dysregulation of PI 3 Kinase Class 2A links to cancer and type 2 diabetes. Alterations in its activity may lead to abnormal cell proliferation and survival characteristics of cancerous transformations associated with proteins like PTEN. Meanwhile its role in glucose metabolism ties it to insulin resistance and diabetes pathogenesis where interactions with insulin receptor substrates become important for disease progression.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

3 product images

  • Western blot - Anti-PI 3 Kinase Class 2A/Cpk antibody [HL1612] - BSA and Azide free (ab313861), expandable thumbnail

    Western blot - Anti-PI 3 Kinase Class 2A/Cpk antibody [HL1612] - BSA and Azide free (ab313861)

    All lanes: Western blot - Anti-PI 3 Kinase Class 2A/Cpk antibody [HL1612] - BSA and Azide free (ab313861) at 1/1000 dilution

    Lane 1: HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell extract at 30 µg

    Lane 2: HepG2 (Human liver hepatocellular carcinoma cell line) whole cell extract at 30 µg

    Secondary

    All lanes: HRP-conjugated anti-rabbit IgG antibody

    Developed using the ECL technique.

  • Western blot - Anti-PI 3 Kinase Class 2A/Cpk antibody [HL1612] - BSA and Azide free (ab313861), expandable thumbnail

    Western blot - Anti-PI 3 Kinase Class 2A/Cpk antibody [HL1612] - BSA and Azide free (ab313861)

    All lanes: Western blot - Anti-PI 3 Kinase Class 2A/Cpk antibody [HL1612] - BSA and Azide free (ab313861) at 1/500 dilution

    Lane 1: Neuro-2a (Mouse neuroblastoma cell line) whole cell extract at 30 µg

    Lane 2: NIH/3T3 (Mouse embryonic fibroblast cell line) whole cell extract at 30 µg

    Lane 3: RAW 264.7 (Mouse macrophage cell line transformed with Abelson murine leukemia virus) whole cell extract at 30 µg

    Lane 4: C2C12 (Mouse myoblast cell line) whole cell extract at 30 µg

    Secondary

    All lanes: HRP-conjugated anti-rabbit IgG antibody

    Predicted band size: 191 kDa

  • Western blot - Anti-PI 3 Kinase Class 2A/Cpk antibody [HL1612] - BSA and Azide free (ab313861), expandable thumbnail

    Western blot - Anti-PI 3 Kinase Class 2A/Cpk antibody [HL1612] - BSA and Azide free (ab313861)

    All lanes: Western blot - Anti-PI 3 Kinase Class 2A/Cpk antibody [HL1612] - BSA and Azide free (ab313861) at 1/500 dilution

    All lanes: PC-12 (Rat adrenal gland pheochromocytoma cell line) whole cell extract at 30 µg

    Secondary

    All lanes: HRP-conjugated anti-rabbit IgG antibody

    Developed using the ECL technique.

    Predicted band size: 191 kDa

Downloads

Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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