AB97862

Anti-PI 3 Kinase p55 gamma antibody

Name: Anti-PI 3 Kinase p55 gamma antibody (ab97862) | Abcam
Brand: Abcam Limited
SKU: AB97862
Price: 455 USD
Availability: InStock

(1 Review)

(3 Publications)

Rabbit Polyclonal PI 3 Kinase p55 gamma antibody. Suitable for WB, IHC-P and reacts with Human samples. Cited in 3 publications. Immunogen corresponding to Recombinant Fragment Protein within Human PIK3R3 aa 200-450.

View Alternative Names

Phosphatidylinositol 3-kinase regulatory subunit gamma, PI3-kinase regulatory subunit gamma, PI3K regulatory subunit gamma, PtdIns-3-kinase regulatory subunit gamma, Phosphatidylinositol 3-kinase 55 kDa regulatory subunit gamma, p55PIK, PI3-kinase subunit p55-gamma, PtdIns-3-kinase regulatory subunit p55-gamma, PIK3R3

2 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PI 3 Kinase p55 gamma antibody (AB97862)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PI 3 Kinase p55 gamma antibody (AB97862)

ab97862 at 1/500 dilution staining PI 3 Kinase p55 gamma in SAS xenograft by Immunohistochemistry, Paraffin-embedded tissue.

Unknown

Western blot - Anti-PI 3 Kinase p55 gamma antibody (AB97862)

7.5% SDS-PAGE.

All lanes:

Western blot - Anti-PI 3 Kinase p55 gamma antibody (ab97862) at 1/1000 dilution

All lanes:

A431 whole cell lysate at 30 µg

Predicted band size: 54 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

WB, IHC-P

applications

Immunogen

Recombinant Fragment Protein within Human PIK3R3 aa 200-450. The exact immunogen used to generate this antibody is proprietary information.

Q92569

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/1000", "IHCP-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Rat": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Cow": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7 Preservative: 0.01% Thimerosal (merthiolate) Constituents: 10% Glycerol (glycerin, glycerine), 1.21% Tris, 0.75% Glycine

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

PI 3 Kinase p55 gamma also known as PIK3R3 is a regulatory subunit of the PI 3-kinase complex. It is a component of the heterodimeric enzyme which includes a catalytic subunit class I and this regulatory subunit. Its molecular mass is around 50-60 kDa. The p55 gamma protein is highly expressed in tissues such as the brain and skeletal muscles. It plays a significant role in intracellular signaling processes mediated by various extracellular signals.

Biological function summary

The p55 gamma protein acts as part of the phosphoinositide 3-kinase (PI3K) complex. This complex transmits signals from growth factors and cytokines contributing to the regulation of cell growth survival and metabolism. The p55 form functions as a regulatory subunit influencing the kinase activity of the catalytic counterpart. It is often found in association with activated receptor tyrosine kinases (RTKs) and aids in the recruitment of the catalytic subunit to the plasma membrane.

Pathways

PI 3 Kinase p55 gamma participates in the PI3K/AKT signaling pathway and the insulin signaling pathway. These pathways are critical for regulating metabolic activities cell growth and survival. It often regulates the activity of AKT also known as Protein Kinase B which in turn affects several downstream signaling proteins and pathways.

The PI 3 Kinase p55 gamma has associations with cancer and insulin resistance due to its role in cell proliferation and metabolic pathways. Abnormal signaling and mutations affecting p55 gamma can lead to oncogenic processes and altered insulin metabolism. The target's interaction with proteins such as AKT makes it a point of interest in understanding cancer pathogenesis and diabetic conditions as disruptions in its signaling can result in abnormal growth and insulin response.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Binds to activated (phosphorylated) protein-tyrosine kinases through its SH2 domain and regulates their kinase activity. During insulin stimulation, it also binds to IRS-1.

See full target information PIK3R3

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Publications (3)

Recent publications for all applications. Explore the full list and refine your search

Journal of experimental & clinical cancer research 39:283 PubMed33317596

2020

CircRNA_0000392 promotes colorectal cancer progression through the miR-193a-5p/PIK3R3/AKT axis.

Applications

Unspecified application

Species

Unspecified reactive species

Hanchen Xu,Yujing Liu,Peiqiu Cheng,Chunyan Wang,Yang Liu,Wenjun Zhou,Yangxian Xu,Guang Ji

Cellular physiology and biochemistry : internation 46:1930-1938 PubMed29719293

2018

PIK3R3 Promotes Metastasis of Pancreatic Cancer via ZEB1 Induced Epithelial-Mesenchymal Transition.

Applications

Unspecified application

Species

Unspecified reactive species

Yun-Peng Peng,Yi Zhu,Ling-Di Yin,Ji-Shu Wei,Xin-Chun Liu,Xiao-Le Zhu,Yi Miao

Clinical cancer research : an official journal of the American Association for Cancer Research 23:575-586 PubMed27503199

2016

Genetic and Methylation-Induced Loss of miR-181a2/181b2 within chr9q33.3 Facilitates Tumor Growth of Cervical Cancer through the PIK3R3/Akt/FoxO Signaling Pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Qian Mei,Xiang Li,Kang Zhang,Zhiqiang Wu,Xiaolei Li,Yuanguang Meng,Mingzhou Guo,Guangbin Luo,Xiaobing Fu,Weidong Han

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-PI 3 Kinase p55 gamma antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PI 3 Kinase p55 gamma antibody (AB97862)

Western blot - Anti-PI 3 Kinase p55 gamma antibody (AB97862)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Alternative Products

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Complementary Products

Primary Antibodies

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Secondary Antibodies

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (3)

Product promise