Rabbit Polyclonal PI 3 Kinase p85 alpha antibody. Suitable for IP, WB, IHC-P and reacts with Human, Mouse, Rat samples. Cited in 19 publications. Immunogen corresponding to Recombinant Fragment Protein within Human PIK3R1.
Images
Publications
Filter by
- Cell death & disease 14:5642023MiR-297 inhibits tumour progression of liver cancer by targeting PTBP3.Applications:Unspecified applicationReactive species:Unspecified reactive speciesNa Lu,Jiali Min,Lin Peng,Shengjian Huang,Xiahua Chai,Susu Wang,Jian WangPubMed 37633911
- Journal of orthopaedic surgery and research 18:212023Study on the treatment of postmenopausal osteoporosis with quercetin in Liuwei Dihuang Pill based on network pharmacology.Applications:Unspecified applicationReactive species:Unspecified reactive speciesFuping Zhu,Wuping Li,Linhua Wang,Bing Dai,Zongyi Liu,Hang Wu,Ting DengPubMed 36624462
- Journal of orthopaedic translation 37:113-1252022Niobium promotes fracture healing in rats by regulating the PI3K-Akt signalling pathway: An and study.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJia Tan,Jiaxin Li,Bojun Cao,Junxiang Wu,Dinghao Luo,Zhaoyang Ran,Liang Deng,Xiaoping Li,Wenbo Jiang,Kai Xie,Lei Wang,Yongqiang HaoPubMed 36262960
- BioMed research international 2022:44697662022Qizhi Kebitong Formula Ameliorates Streptozocin-Induced Diabetic Osteoporosis through Regulating the PI3K/Akt/NF-B Pathway.Applications:Unspecified applicationReactive species:Unspecified reactive speciesLulu Tian,Lu Ding,Guoqiang Wang,Yu Guo,Yunyun Zhao,Yuchi Wei,Xingquan Li,Wei Zhang,Jia Mi,Xiangyan Li,Zeyu Wang,Xiuge WangPubMed 36046447
- Bioengineered 13:9345-93562022Fraxetin down-regulates polo-like kinase 4 (PLK4) to inhibit proliferation, migration and invasion of prostate cancer cells through the phosphatidylinositol 3-kinase (PI3K)/protein kinase B (Akt) pathway.Applications:Unspecified applicationReactive species:Unspecified reactive speciesZheng Ma,Yanfang Sun,Weixing PengPubMed 35387563
- Frontiers in immunology 12:7272542021PD-1 Blockade Restores the Proliferation of Peripheral Blood Lymphocyte and Inhibits Lymphocyte Apoptosis in a BALB/c Mouse Model of CP BVDV Acute Infection.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYu Liu,Chenhua Wu,Nannan Chen,Yang Li,Chunling Fan,Shangqi Zhao,Tongtong Bai,Zhibo Zhao,Jinwei Chen,Siyu Su,Zecai Zhang,Yulong Zhou,Zhanbo ZhuPubMed 34552590
- Stem cell research & therapy 12:4132021Hypoxic preconditioning rejuvenates mesenchymal stem cells and enhances neuroprotection following intracerebral hemorrhage via the miR-326-mediated autophagy.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJianyang Liu,Jialin He,Lite Ge,Han Xiao,Yan Huang,Liuwang Zeng,Zheng Jiang,Ming Lu,Zhiping HuPubMed 34294127
- Molecular medicine reports 23:2021Apatinib suppresses the migration, invasion and angiogenesis of hepatocellular carcinoma cells by blocking VEGF and PI3K/AKT signaling pathways.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJifu Song,Zhibin Guan,Chao Song,Maojiang Li,Zhiwei Gao,Yongli ZhaoPubMed 33846786
- International journal of biological sciences 17:1289-13012021Enforced expression of miR-92b blunts lipopolysaccharide-mediated inflammatory injury by activating the PI3K/AKT/β-catenin pathway via targeting PTEN.Applications:Unspecified applicationReactive species:Unspecified reactive speciesKangfeng Jiang,Jing Yang,Chunlian Song,Fengping He,Liangyu Yang,Xiaobing LiPubMed 33867846
- Evidence-based complementary and alternative medicine : eCAM 2021:88468132021Epigallocatechin Gallate Protects against MNNG-Induced Precancerous Lesions of Gastric Carcinoma in Rats via PI3K/Akt/mTOR Pathway.Applications:Unspecified applicationReactive species:Unspecified reactive speciesFeiye Zhu,Yanli Xu,Jieli Pan,Meiya Li,Fangming Chen,Guanqun XiePubMed 33628319
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 79.99% PBS, 20% Glycerol (glycerin, glycerine)- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Recombinant Fragment Protein within Human PIK3R1. The exact immunogen used to generate this antibody is proprietary information. Database link P27986
Consider this alternative
Reactivity data
Select an application| IP | WB | IHC-P | |
|---|---|---|---|
| Human | Tested | Tested | Expected |
| Mouse | Expected | Tested | Tested |
| Rat | Expected | Tested | Expected |
| Cow | Predicted | Predicted | Predicted |
| Rhesus monkey | Predicted | Predicted | Predicted |
IP
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/100 - 1/500 | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info Use at an assay dependent concentration. | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Cow, Rhesus monkey | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 1/500 - 1/3000 | Notes - |
Species Rat | Dilution info 1/500 - 1/3000 | Notes - |
Species Human | Dilution info 1/500 - 1/3000 | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Cow, Rhesus monkey | Dilution info - | Notes - |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 1/100 - 1/1000 | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Rat | Dilution info Use at an assay dependent concentration. | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Cow, Rhesus monkey | Dilution info - | Notes - |
Associated Products
Select an associated product type
7 products for Alternative Product
Target data
Function
Binds to activated (phosphorylated) protein-Tyr kinases, through its SH2 domain, and acts as an adapter, mediating the association of the p110 catalytic unit to the plasma membrane. Necessary for the insulin-stimulated increase in glucose uptake and glycogen synthesis in insulin-sensitive tissues. Plays an important role in signaling in response to FGFR1, FGFR2, FGFR3, FGFR4, KITLG/SCF, KIT, PDGFRA and PDGFRB. Likewise, plays a role in ITGB2 signaling (PubMed:17626883, PubMed:19805105, PubMed:7518429). Modulates the cellular response to ER stress by promoting nuclear translocation of XBP1 isoform 2 in a ER stress- and/or insulin-dependent manner during metabolic overloading in the liver and hence plays a role in glucose tolerance improvement (PubMed:20348923).
Alternative names
GRB1, PIK3R1, Phosphatidylinositol 3-kinase regulatory subunit alpha, PI3-kinase regulatory subunit alpha, PI3K regulatory subunit alpha, PtdIns-3-kinase regulatory subunit alpha, Phosphatidylinositol 3-kinase 85 kDa regulatory subunit alpha, PI3-kinase subunit p85-alpha, PtdIns-3-kinase regulatory subunit p85-alpha
Recommended products
Rabbit Polyclonal PI 3 Kinase p85 alpha antibody. Suitable for IP, WB, IHC-P and reacts with Human, Mouse, Rat samples. Cited in 19 publications. Immunogen corresponding to Recombinant Fragment Protein within Human PIK3R1.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 79.99% PBS, 20% Glycerol (glycerin, glycerine)- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Recombinant Fragment Protein within Human PIK3R1. The exact immunogen used to generate this antibody is proprietary information. Database link P27986
- Purification technique
- Affinity purification Immunogen
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.
Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
PI 3 Kinase p85 alpha also known as PI3KR1 or phospho PI3K is a regulatory subunit of phosphoinositide 3-kinase (PI3K) that plays a fundamental role in various signaling pathways. The p85 protein has a molecular weight of approximately 85 kDa. This protein is widely expressed in many cell types facilitating essential cellular functions by forming a heterodimer with the class I PI3K catalytic subunit often implicated in regulating lipid signaling pathways.
- Biological function summary
P85 alpha modulates intracellular signaling by interacting with other proteins in a complex formation with catalytic subunit p110. This interaction enables the initiation of downstream signaling cascades controlling cell growth proliferation and survival. PI 3 kinase activity mediated by p85 alpha is integral to cellular homeostasis and metabolism helping to execute cellular responses to a variety of growth factors and hormones.
- Pathways
PI 3 Kinase p85 alpha integrates into two principal signaling networks: the PI3K-Akt and mTOR pathways. These pathways are instrumental for regulating anabolic processes and cell survival. The p85 form of PI3K interacts with additional proteins such as Akt and mTOR coordinating cellular energy balance promoting protein synthesis and inhibiting apoptosis. Through these interactions the protein ensures that cells respond accurately to nutritional and stress signals.
- Associated diseases and disorders
Alterations in PI 3 Kinase p85 alpha function have been linked to cancer and type 2 diabetes. Aberrant signaling involving PI3K-Akt-mTOR pathways due to mutations in PI3KR1 can contribute to tumor progression and chemoresistance. Moreover dysfunction of this pathway affects insulin signaling connecting p85 alpha to diabetic pathologies. Proteins such as IRS-1 (insulin receptor substrate 1) act alongside p85 alpha in mediating these disease-associated processes emphasizing its importance in maintaining physiological balance.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
5 product images
Western blot - Anti-PI 3 Kinase p85 alpha antibody (ab227204)
7.5% SDS-PAGE
All lanes: Western blot - Anti-PI 3 Kinase p85 alpha antibody (ab227204) at 1/1000 dilution
Lane 1: Jurkat (human T cell leukemia cell line from peripheral blood) whole cell extract at 30 µg
Lane 2: K562 (human chronic myelogenous leukemia cell line from bone marrow ) whole cell extract at 30 µg
Lane 3: THP-1 (human monocytic leukemia cell line) whole cell extract at 30 µg
SecondaryAll lanes: HRP-conjugated anti-rabbit IgG
Developed using the ECL technique.
Predicted band size: 83 kDa
Immunoprecipitation - Anti-PI 3 Kinase p85 alpha antibody (ab227204)
PI 3 Kinase p85 alpha was immunoprecipitated from Jurkat (human T cell leukemia cell line from peripheral blood) whole cell extract with 5 μg ab227204. Western blot was performed from the immunoprecipitate using ab227204 at 1/500 dilution.
Lane 1: Control IgG IP in Jurkat whole cell extract.
Lane 2: ab227204 IP in Jurkat whole cell extract.
10% SDS-PAGE.
All lanes: Immunoprecipitation - Anti-PI 3 Kinase p85 alpha antibody (ab227204)
Developed using the ECL technique.
Predicted band size: 83 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PI 3 Kinase p85 alpha antibody (ab227204)
Paraffin-embedded mouse duodenum stained for PI 3 Kinase p85 alpha with ab227204 at 1/500 dilution in immunohistochemical analysis.
Western blot - Anti-PI 3 Kinase p85 alpha antibody (ab227204)
7.5% SDS-PAGE
All lanes: Western blot - Anti-PI 3 Kinase p85 alpha antibody (ab227204) at 1/500 dilution
Lane 1: Neuro-2a (mouse neuroblastoma cell line) whole cell extract at 30 µg
Lane 2: C8D30 whole cell extract at 30 µg
Lane 3: NIH/3T3 (mouse embryo fibroblast cell line) whole cell extract at 30 µg
Lane 4: Raw 264.7 (mouse macrophage cell line transformed with Abelson murine leukemia virus) whole cell extract at 30 µg
Lane 5: C2C12 (mouse myoblast cell line) whole cell extract at 30 µg
SecondaryAll lanes: HRP-conjugated anti-rabbit IgG
Developed using the ECL technique.
Predicted band size: 83 kDa
Western blot - Anti-PI 3 Kinase p85 alpha antibody (ab227204)
7.5% SDS-PAGE
All lanes: Western blot - Anti-PI 3 Kinase p85 alpha antibody (ab227204) at 1/1000 dilution
Lane 1: PC-12 (rat adrenal gland pheochromocytoma cell line) whole cell extract at 30 µg
Lane 2: Rat2 (rat fibroblast cell line) whole cell extract at 30 µg
SecondaryAll lanes: HRP-conjugated anti-rabbit IgG
Developed using the ECL technique.
Predicted band size: 83 kDa
Downloads
Product protocols
- Visit the general protocols
- Visit troubleshooting
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
For licensing inquiries, please contact partnerships@abcam.com