AB40755

Anti-PI 3 Kinase p85 alpha antibody [EP380Y]

Name: Anti-PI 3 Kinase p85 alpha antibody [EP380Y] (ab40755) | Abcam
Brand: Abcam Limited
SKU: AB40755
Price: 200 USD
Availability: InStock
Rating: 4 (3 reviews)

20ul selling size
RabMAb
Recombinant
KO Validated
What is this?

(3 Reviews)

(51 Publications)

Rabbit Recombinant Monoclonal PI 3 Kinase p85 alpha antibody. Suitable for WB and reacts with Human, Mouse, Rat samples. Cited in 51 publications.

View Alternative Names

GRB1, PIK3R1, Phosphatidylinositol 3-kinase regulatory subunit alpha, PI3-kinase regulatory subunit alpha, PI3K regulatory subunit alpha, PtdIns-3-kinase regulatory subunit alpha, Phosphatidylinositol 3-kinase 85 kDa regulatory subunit alpha, PI3-kinase subunit p85-alpha, PtdIns-3-kinase regulatory subunit p85-alpha

3 Images

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Western blot - Anti-PI 3 Kinase p85 alpha antibody [EP380Y] (AB40755)

All lanes:

Western blot - Anti-PI 3 Kinase p85 alpha antibody [EP380Y] (ab40755) at 1/2000 dilution

Lane 1:

A431 cell lysate at 10 µg

Lane 2:

MCF-7 cell lysate at 10 µg

Lane 3:

Raji cell lysate at 10 µg

Predicted band size: 83 kDa

Observed band size: 85 kDa

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Lab

Western blot - Anti-PI 3 Kinase p85 alpha antibody [EP380Y] (AB40755)

Lane 1 : Wild-type HAP1 cell lysate (20 μg)
Lane 2 : PI 3 Kinase p85 alpha knockout HAP1 cell lysate (20 μg)
Lane 3 : Jurkat cell lysate (20 μg)
Lane 4 : THP1 cell lysate (20 μg)
Lanes 1 - 4 : Merged signal (red and green). Green - ab40755 observed at 90 kDa. Red - loading control, ab181602, observed at 37 kDa.

ab40755 was shown to recognize PI 3 Kinase p85 alpha in wild-type HAP1 cells along with additional cross-reactive bands. No Band was observed when PI 3 Kinase p85 alpha knockout samples were examined. Wild-type and PI 3 Kinase p85 alpha knockout samples were subjected to SDS-PAGE. ab40755 and ab181602 (loading control to GAPDH) were diluted 1/1000 and 1/2000 respectively and incubated overnight at 4°C. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye^® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye^® 680RD) preadsorbed (ab216776) secondary antibodies at 1/10,000 dilution for 1 hour at room temperature before imaging.

All lanes:

Western blot - Anti-PI 3 Kinase p85 alpha antibody [EP380Y] (ab40755)

Predicted band size: 83 kDa

false

Lab

Western blot - Anti-PI 3 Kinase p85 alpha antibody [EP380Y] (AB40755)

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

Lysates were freshly made and used for Western blotting immediately to minimize protein degradation.

Compared with ab40755, ab191606 has higher sensitivity. We recommend ab124805 as an alternative for testing western blot.

We suggest optimizing experimental protocols (increasing lysate amount, using lower dilution or higher sensitivity ECL substrate) to improve results when using ab197896 in western blot.

Lane 1:

Western blot - Anti-PI 3 Kinase p85 alpha antibody [EP380Y] (ab40755) at 1/1000 dilution

Lane 2:

Western blot - Anti-PI 3 Kinase p85 alpha antibody [EPR18702] (<a href='/en-us/products/primary-antibodies/pi-3-kinase-p85-alpha-antibody-epr18702-ab191606'>ab191606</a>) at 1/1000 dilution

All lanes:

Jurkat (human T cell leukemia T lymphocyte) whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Observed band size: 85 kDa

false

Exposure time: 40s

Carrier free

Anti-PI 3 Kinase p85 alpha antibody [EP380Y] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EP380Y

Isotype

IgG

Carrier free

Reacts with

Human

Applications

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/2000", "WB-species-notes": "" }, "Mouse": { "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rat": { "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

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Product details

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Preservative: 0.05% Sodium azide Constituents: 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

PI 3 Kinase p85 alpha also known as PI3KR1 or phospho PI3K is a regulatory subunit of phosphoinositide 3-kinase (PI3K) that plays a fundamental role in various signaling pathways. The p85 protein has a molecular weight of approximately 85 kDa. This protein is widely expressed in many cell types facilitating essential cellular functions by forming a heterodimer with the class I PI3K catalytic subunit often implicated in regulating lipid signaling pathways.

Biological function summary

P85 alpha modulates intracellular signaling by interacting with other proteins in a complex formation with catalytic subunit p110. This interaction enables the initiation of downstream signaling cascades controlling cell growth proliferation and survival. PI 3 kinase activity mediated by p85 alpha is integral to cellular homeostasis and metabolism helping to execute cellular responses to a variety of growth factors and hormones.

Pathways

PI 3 Kinase p85 alpha integrates into two principal signaling networks: the PI3K-Akt and mTOR pathways. These pathways are instrumental for regulating anabolic processes and cell survival. The p85 form of PI3K interacts with additional proteins such as Akt and mTOR coordinating cellular energy balance promoting protein synthesis and inhibiting apoptosis. Through these interactions the protein ensures that cells respond accurately to nutritional and stress signals.

Alterations in PI 3 Kinase p85 alpha function have been linked to cancer and type 2 diabetes. Aberrant signaling involving PI3K-Akt-mTOR pathways due to mutations in PI3KR1 can contribute to tumor progression and chemoresistance. Moreover dysfunction of this pathway affects insulin signaling connecting p85 alpha to diabetic pathologies. Proteins such as IRS-1 (insulin receptor substrate 1) act alongside p85 alpha in mediating these disease-associated processes emphasizing its importance in maintaining physiological balance.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Binds to activated (phosphorylated) protein-Tyr kinases, through its SH2 domain, and acts as an adapter, mediating the association of the p110 catalytic unit to the plasma membrane. Necessary for the insulin-stimulated increase in glucose uptake and glycogen synthesis in insulin-sensitive tissues. Plays an important role in signaling in response to FGFR1, FGFR2, FGFR3, FGFR4, KITLG/SCF, KIT, PDGFRA and PDGFRB. Likewise, plays a role in ITGB2 signaling (PubMed : 17626883, PubMed : 19805105, PubMed : 7518429). Modulates the cellular response to ER stress by promoting nuclear translocation of XBP1 isoform 2 in a ER stress- and/or insulin-dependent manner during metabolic overloading in the liver and hence plays a role in glucose tolerance improvement (PubMed : 20348923).

See full target information PIK3R1

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Publications (51)

Recent publications for all applications. Explore the full list and refine your search

Cell division 20:16 PubMed40563088

2025

Mitochondrial fission regulator 2 promotes cell proliferation, migration and invasion in hepatocellular carcinoma through regulating PI3K/AKT signaling pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Qiang Sun,Kaikun Liu,Fangjie Zheng,Xinwei Chen

Phytotherapy research : PTR 37:4133-4148 PubMed37189016

2023

Astragaloside IV attenuates high glucose-induced NF-κB-mediated inflammation through activation of PI3K/AKT-ERK-dependent Nrf2/ARE signaling pathway in glomerular mesangial cells.

Applications

Unspecified application

Species

Unspecified reactive species

Xue Su,Hengjiang Guo,Yuying Zhou,Aili Cao,Qian Shen,Bingbing Zhu,Xingmei Yao,Yunman Wang,Hao Wang,Li Wang

Cell calcium 111:102718 PubMed36934559

2023

p85α regulatory subunit isoform controls PI3-kinase and TRPC6 membrane translocation.

Applications

Unspecified application

Species

Unspecified reactive species

Pinaki Chaudhuri,Priya Putta,Linda M Graham,Michael A Rosenbaum

iScience 26:105923 PubMed36685035

2023

Streptococcus pneumoniae promotes lung cancer development and progression.

Applications

Unspecified application

Species

Unspecified reactive species

Ning Li,Huifen Zhou,Van K Holden,Janaki Deepak,Pushpa Dhilipkannah,Nevins W Todd,Sanford A Stass,Feng Jiang

Nutrients 14: PubMed36432439

2022

Isoflavone-Enriched Soybean Leaves (Glycine Max) Alleviate Cognitive Impairment Induced by Ovariectomy and Modulate PI3K/Akt Signaling in the Hippocampus of C57BL6 Mice.

Applications

Unspecified application

Species

Unspecified reactive species

Dae Young Yoo,Soonwoong Jung,Jae Soon Kang,Ji Hyeong Baek,Ki Hun Park,Dong Hoon Lee,Sang Soo Kang,Hyun Joon Kim

Oncology reports 48: PubMed35920185

2022

Synergistic effects of thalidomide and cisplatin are mediated via the PI3K/AKT and JAK1/STAT3 signaling pathways in cervical cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Cairu Liu,Haiqin Feng,Lihong Song,Shuirui Li,Yiping Wu,Liping Yang

Frontiers in aging neuroscience 14:889983 PubMed35656538

2022

Intermittent Theta Burst Stimulation Ameliorates Cognitive Deficit and Attenuates Neuroinflammation PI3K/Akt/mTOR Signaling Pathway in Alzheimer's-Like Disease Model.

Applications

Unspecified application

Species

Unspecified reactive species

Andjela Stekic,Milica Zeljkovic,Marina Zaric Kontic,Katarina Mihajlovic,Marija Adzic,Ivana Stevanovic,Milica Ninkovic,Ivana Grkovic,Tihomir V Ilic,Nadezda Nedeljkovic,Milorad Dragic

Journal of Cancer 13:1837-1847 PubMed35399731

2022

LPCAT1 promotes gefitinib resistance via upregulation of the EGFR/PI3K/AKT signaling pathway in lung adenocarcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Jianghua Ding,Xinjing Ding,Zhaohui Leng

Neurotoxicity research 40:154-172 PubMed34997457

2022

Involvement of FoxO1, Sp1, and Nrf2 in Upregulation of Negative Regulator of ROS by 15d-PGJ Attenuates HO-Induced IL-6 Expression in Rat Brain Astrocytes.

Applications

Unspecified application

Species

Unspecified reactive species

Chen-Yu Wang,Chien-Chung Yang,Li-Der Hsiao,Chuen-Mao Yang

Oxidative medicine and cellular longevity 2021:4576071 PubMed34422208

2021

Ameliorative Effects and Mechanism of Buyang Huanwu Decoction on Pulmonary Vascular Remodeling: Network and Experimental Analyses.

Applications

Unspecified application

Species

Unspecified reactive species

Yucai Chen,Lidan Cui,Can Wang,Jianing Liu,Jian Guo

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-PI 3 Kinase p85 alpha antibody [EP380Y]

Western blot - Anti-PI 3 Kinase p85 alpha antibody [EP380Y] (AB40755)

Western blot - Anti-PI 3 Kinase p85 alpha antibody [EP380Y] (AB40755)

Western blot - Anti-PI 3 Kinase p85 alpha antibody [EP380Y] (AB40755)

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Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (51)

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