Rabbit Recombinant Monoclonal PI 3 Kinase p85 alpha antibody. Carrier free. Suitable for WB and reacts with Human samples.
Images
![Western blot - Anti-PI 3 Kinase p85 alpha antibody [EP380Y] - BSA and Azide free (AB182617), expandable thumbnail](https://content.abcam.com/products/images/pi-3-kinase-p85-alpha-antibody-ep380y-bsa-and-azide-free-ab182617--western-blot-img7174.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-PI 3 Kinase p85 alpha antibody [EP380Y] - BSA and Azide free (AB182617)")
![Western blot - Anti-PI 3 Kinase p85 alpha antibody [EP380Y] - BSA and Azide free (AB182617), expandable thumbnail](https://content.abcam.com/products/images/pi-3-kinase-p85-alpha-antibody-ep380y-bsa-and-azide-free-ab182617--western-blot-img18043.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-PI 3 Kinase p85 alpha antibody [EP380Y] - BSA and Azide free (AB182617)")
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| WB | |
|---|---|
| Human | Tested |
| Mouse | Predicted |
| Rat | Predicted |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info - | Notes - |
Associated Products
Select an associated product type
1 product for Alternative Product
Target data
Function
Binds to activated (phosphorylated) protein-Tyr kinases, through its SH2 domain, and acts as an adapter, mediating the association of the p110 catalytic unit to the plasma membrane. Necessary for the insulin-stimulated increase in glucose uptake and glycogen synthesis in insulin-sensitive tissues. Plays an important role in signaling in response to FGFR1, FGFR2, FGFR3, FGFR4, KITLG/SCF, KIT, PDGFRA and PDGFRB. Likewise, plays a role in ITGB2 signaling (PubMed:17626883, PubMed:19805105, PubMed:7518429). Modulates the cellular response to ER stress by promoting nuclear translocation of XBP1 isoform 2 in a ER stress- and/or insulin-dependent manner during metabolic overloading in the liver and hence plays a role in glucose tolerance improvement (PubMed:20348923).
Alternative names
GRB1, PIK3R1, Phosphatidylinositol 3-kinase regulatory subunit alpha, PI3-kinase regulatory subunit alpha, PI3K regulatory subunit alpha, PtdIns-3-kinase regulatory subunit alpha, Phosphatidylinositol 3-kinase 85 kDa regulatory subunit alpha, PI3-kinase subunit p85-alpha, PtdIns-3-kinase regulatory subunit p85-alpha
Recommended products
Rabbit Recombinant Monoclonal PI 3 Kinase p85 alpha antibody. Carrier free. Suitable for WB and reacts with Human samples.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Carrier free
- Yes
- Clone number
- EP380Y
- Purification technique
- Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
- Storage information
- Do Not Freeze
Notes
ab182617 is the carrier-free version of Anti-PI 3 Kinase p85 alpha antibody [EP380Y] ab40755.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
PI 3 Kinase p85 alpha also known as PI3KR1 or phospho PI3K is a regulatory subunit of phosphoinositide 3-kinase (PI3K) that plays a fundamental role in various signaling pathways. The p85 protein has a molecular weight of approximately 85 kDa. This protein is widely expressed in many cell types facilitating essential cellular functions by forming a heterodimer with the class I PI3K catalytic subunit often implicated in regulating lipid signaling pathways.
- Biological function summary
P85 alpha modulates intracellular signaling by interacting with other proteins in a complex formation with catalytic subunit p110. This interaction enables the initiation of downstream signaling cascades controlling cell growth proliferation and survival. PI 3 kinase activity mediated by p85 alpha is integral to cellular homeostasis and metabolism helping to execute cellular responses to a variety of growth factors and hormones.
- Pathways
PI 3 Kinase p85 alpha integrates into two principal signaling networks: the PI3K-Akt and mTOR pathways. These pathways are instrumental for regulating anabolic processes and cell survival. The p85 form of PI3K interacts with additional proteins such as Akt and mTOR coordinating cellular energy balance promoting protein synthesis and inhibiting apoptosis. Through these interactions the protein ensures that cells respond accurately to nutritional and stress signals.
- Associated diseases and disorders
Alterations in PI 3 Kinase p85 alpha function have been linked to cancer and type 2 diabetes. Aberrant signaling involving PI3K-Akt-mTOR pathways due to mutations in PI3KR1 can contribute to tumor progression and chemoresistance. Moreover dysfunction of this pathway affects insulin signaling connecting p85 alpha to diabetic pathologies. Proteins such as IRS-1 (insulin receptor substrate 1) act alongside p85 alpha in mediating these disease-associated processes emphasizing its importance in maintaining physiological balance.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
2 product images
![Western blot - Anti-PI 3 Kinase p85 alpha antibody [EP380Y] - BSA and Azide free (ab182617), expandable thumbnail](https://content.abcam.com/products/images/pi-3-kinase-p85-alpha-antibody-ep380y-bsa-and-azide-free-ab182617--western-blot-img7174.jpg "Western blot - Anti-PI 3 Kinase p85 alpha antibody [EP380Y] - BSA and Azide free (ab182617)")
Western blot - Anti-PI 3 Kinase p85 alpha antibody [EP380Y] - BSA and Azide free (ab182617)
This data was developed using Anti-PI 3 Kinase p85 alpha antibody [EP380Y] ab40755, the same antibody clone in a different buffer formulation.
Lane 1: Wild-type HAP1 cell lysate (20 μg)
Lane 2: PI 3 Kinase p85 alpha knockout HAP1 cell lysate (20 μg)
Lane 3: Jurkat cell lysate (20 μg)
Lane 4: THP1 cell lysate (20 μg)
Lanes 1 - 4: Merged signal (red and green). Green - Anti-PI 3 Kinase p85 alpha antibody [EP380Y] ab40755 observed at 90 kDa. Red - loading control, Anti-GAPDH antibody [EPR16891] - Loading Control ab181602, observed at 37 kDa.
Anti-PI 3 Kinase p85 alpha antibody [EP380Y] ab40755 was shown to recognize PI 3 Kinase p85 alpha in wild-type HAP1 cells along with additional cross-reactive bands. No Band was observed when PI 3 Kinase p85 alpha knockout samples were examined. Wild-type and PI 3 Kinase p85 alpha knockout samples were subjected to SDS-PAGE. Anti-PI 3 Kinase p85 alpha antibody [EP380Y] ab40755 and Anti-GAPDH antibody [EPR16891] - Loading Control ab181602 (loading control to GAPDH) were diluted 1/1000 and 1/2000 respectively and incubated overnight at 4°C. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776) secondary antibodies at 1/10,000 dilution for 1 hour at room temperature before imaging.
All lanes: Western blot - Anti-PI 3 Kinase p85 alpha antibody [EP380Y] (Anti-PI 3 Kinase p85 alpha antibody [EP380Y] ab40755)
Predicted band size: 83 kDa
![Western blot - Anti-PI 3 Kinase p85 alpha antibody [EP380Y] - BSA and Azide free (ab182617), expandable thumbnail](https://content.abcam.com/products/images/pi-3-kinase-p85-alpha-antibody-ep380y-bsa-and-azide-free-ab182617--western-blot-img18043.jpg "Western blot - Anti-PI 3 Kinase p85 alpha antibody [EP380Y] - BSA and Azide free (ab182617)")
Western blot - Anti-PI 3 Kinase p85 alpha antibody [EP380Y] - BSA and Azide free (ab182617)
This data was developed using Anti-PI 3 Kinase p85 alpha antibody [EP380Y] ab40755, the same antibody clone in a different buffer formulation.
All lanes: Western blot - Anti-PI 3 Kinase p85 alpha antibody [EP380Y] (Anti-PI 3 Kinase p85 alpha antibody [EP380Y] ab40755) at 1/2000 dilution
Lane 1: A431 cell lysate at 10 µg
Lane 2: MCF-7 cell lysate at 10 µg
Lane 3: Raji cell lysate at 10 µg
Predicted band size: 83 kDa
Observed band size: 85 kDa
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