Rabbit Recombinant Monoclonal PI 3 Kinase p85 alpha antibody. Carrier free. Suitable for WB, ICC/IF, IP, Flow Cyt (Intra) and reacts with Human, Mouse, Rat, African green monkey samples. Cited in 1 publication.
Images
![Western blot - Anti-PI 3 Kinase p85 alpha antibody [EPR18702] - BSA and Azide free (AB223792), expandable thumbnail](https://content.abcam.com/products/images/pi-3-kinase-p85-alpha-antibody-epr18702-bsa-and-azide-free-ab223792--western-blot-img136882.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-PI 3 Kinase p85 alpha antibody [EPR18702] - BSA and Azide free (AB223792)")
![Immunocytochemistry/ Immunofluorescence - Anti-PI 3 Kinase p85 alpha antibody [EPR18702] - BSA and Azide free (AB223792), expandable thumbnail](https://content.abcam.com/products/images/pi-3-kinase-p85-alpha-antibody-epr18702-bsa-and-azide-free-ab223792--immunocytochemistry-immunofluorescence-img66131.jpg/_jcr_content/renditions/webp-475.webp "Immunocytochemistry/ Immunofluorescence - Anti-PI 3 Kinase p85 alpha antibody [EPR18702] - BSA and Azide free (AB223792)")
![Western blot - Anti-PI 3 Kinase p85 alpha antibody [EPR18702] - BSA and Azide free (AB223792), expandable thumbnail](https://content.abcam.com/products/images/pi-3-kinase-p85-alpha-antibody-epr18702-bsa-and-azide-free-ab223792--western-blot-img54820.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-PI 3 Kinase p85 alpha antibody [EPR18702] - BSA and Azide free (AB223792)")
![Immunocytochemistry/ Immunofluorescence - Anti-PI 3 Kinase p85 alpha antibody [EPR18702] - BSA and Azide free (AB223792), expandable thumbnail](https://content.abcam.com/products/images/pi-3-kinase-p85-alpha-antibody-epr18702-bsa-and-azide-free-ab223792--immunocytochemistry-immunofluorescence-img71196.jpg/_jcr_content/renditions/webp-475.webp "Immunocytochemistry/ Immunofluorescence - Anti-PI 3 Kinase p85 alpha antibody [EPR18702] - BSA and Azide free (AB223792)")
![Flow Cytometry (Intracellular) - Anti-PI 3 Kinase p85 alpha antibody [EPR18702] - BSA and Azide free (AB223792), expandable thumbnail](https://content.abcam.com/products/images/pi-3-kinase-p85-alpha-antibody-epr18702-bsa-and-azide-free-ab223792--flow-cytometry-intracellular-img26647.jpg/_jcr_content/renditions/webp-475.webp "Flow Cytometry (Intracellular) - Anti-PI 3 Kinase p85 alpha antibody [EPR18702] - BSA and Azide free (AB223792)")
Publications
Filter by
- Cancers 15:2023Flexible Cyclic Immunofluorescence (cyCIF) Using Oligonucleotide Barcoded Antibodies.Applications:Unspecified applicationReactive species:Unspecified reactive speciesNathan P McMahon,Jocelyn A Jones,Ashley N Anderson,Matthew S Dietz,Melissa H Wong,Summer L GibbsPubMed 36765785
Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
Liquid
- Clonality
Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| WB | ICC/IF | IP | Flow Cyt (Intra) | |
|---|---|---|---|---|
| Human | Tested | Tested | Tested | Expected |
| Mouse | Tested | Tested | Expected | Tested |
| Rat | Tested | Expected | Expected | Expected |
| African green monkey | Expected | Expected | Expected | Tested |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Mouse, Rat | Dilution info - | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species African green monkey | Dilution info Use at an assay dependent concentration. | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Mouse | Dilution info - | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Rat, African green monkey | Dilution info Use at an assay dependent concentration. | Notes - |
IP
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat, African green monkey | Dilution info Use at an assay dependent concentration. | Notes - |
Flow Cyt (Intra)
TestedTested
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Rat | Dilution info Use at an assay dependent concentration. | Notes - |
Associated Products
Select an associated product type
8 products for Alternative Product
3 products for Alternative Version
Target data
Function
Binds to activated (phosphorylated) protein-Tyr kinases, through its SH2 domain, and acts as an adapter, mediating the association of the p110 catalytic unit to the plasma membrane. Necessary for the insulin-stimulated increase in glucose uptake and glycogen synthesis in insulin-sensitive tissues. Plays an important role in signaling in response to FGFR1, FGFR2, FGFR3, FGFR4, KITLG/SCF, KIT, PDGFRA and PDGFRB. Likewise, plays a role in ITGB2 signaling (PubMed:17626883, PubMed:19805105, PubMed:7518429). Modulates the cellular response to ER stress by promoting nuclear translocation of XBP1 isoform 2 in a ER stress- and/or insulin-dependent manner during metabolic overloading in the liver and hence plays a role in glucose tolerance improvement (PubMed:20348923).
Alternative names
Phosphatidylinositol 3-kinase regulatory subunit alpha, PI3-kinase regulatory subunit alpha, PI3K regulatory subunit alpha, PtdIns-3-kinase regulatory subunit alpha, Phosphatidylinositol 3-kinase 85 kDa regulatory subunit alpha, PI3-kinase subunit p85-alpha, PtdIns-3-kinase regulatory subunit p85-alpha, PIK3R1, GRB1
Recommended products
Rabbit Recombinant Monoclonal PI 3 Kinase p85 alpha antibody. Carrier free. Suitable for WB, ICC/IF, IP, Flow Cyt (Intra) and reacts with Human, Mouse, Rat, African green monkey samples. Cited in 1 publication.
Alternative names
Phosphatidylinositol 3-kinase regulatory subunit alpha, PI3-kinase regulatory subunit alpha, PI3K regulatory subunit alpha, PtdIns-3-kinase regulatory subunit alpha, Phosphatidylinositol 3-kinase 85 kDa regulatory subunit alpha, PI3-kinase subunit p85-alpha, PtdIns-3-kinase regulatory subunit p85-alpha, PIK3R1, GRB1
Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
Liquid
- Clonality
Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Carrier free
Yes
- Clone number
EPR18702
- Purification technique
Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
Blue Ice
- Appropriate long-term storage conditions
+4°C
- Storage information
Do Not Freeze
Notes
ab223792 is the carrier-free version of Anti-PI 3 Kinase p85 alpha antibody [EPR18702] ab191606.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
Supplementary info
This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
PI 3 Kinase p85 alpha also known as PI3KR1 or phospho PI3K is a regulatory subunit of phosphoinositide 3-kinase (PI3K) that plays a fundamental role in various signaling pathways. The p85 protein has a molecular weight of approximately 85 kDa. This protein is widely expressed in many cell types facilitating essential cellular functions by forming a heterodimer with the class I PI3K catalytic subunit often implicated in regulating lipid signaling pathways.
- Biological function summary
P85 alpha modulates intracellular signaling by interacting with other proteins in a complex formation with catalytic subunit p110. This interaction enables the initiation of downstream signaling cascades controlling cell growth proliferation and survival. PI 3 kinase activity mediated by p85 alpha is integral to cellular homeostasis and metabolism helping to execute cellular responses to a variety of growth factors and hormones.
- Pathways
PI 3 Kinase p85 alpha integrates into two principal signaling networks: the PI3K-Akt and mTOR pathways. These pathways are instrumental for regulating anabolic processes and cell survival. The p85 form of PI3K interacts with additional proteins such as Akt and mTOR coordinating cellular energy balance promoting protein synthesis and inhibiting apoptosis. Through these interactions the protein ensures that cells respond accurately to nutritional and stress signals.
- Associated diseases and disorders
Alterations in PI 3 Kinase p85 alpha function have been linked to cancer and type 2 diabetes. Aberrant signaling involving PI3K-Akt-mTOR pathways due to mutations in PI3KR1 can contribute to tumor progression and chemoresistance. Moreover dysfunction of this pathway affects insulin signaling connecting p85 alpha to diabetic pathologies. Proteins such as IRS-1 (insulin receptor substrate 1) act alongside p85 alpha in mediating these disease-associated processes emphasizing its importance in maintaining physiological balance.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
7 product images
![Western blot - Anti-PI 3 Kinase p85 alpha antibody [EPR18702] - BSA and Azide free (ab223792), expandable thumbnail](https://content.abcam.com/products/images/pi-3-kinase-p85-alpha-antibody-epr18702-bsa-and-azide-free-ab223792--western-blot-img136882.jpg "Western blot - Anti-PI 3 Kinase p85 alpha antibody [EPR18702] - BSA and Azide free (ab223792)")
Western blot - Anti-PI 3 Kinase p85 alpha antibody [EPR18702] - BSA and Azide free (ab223792)
This WB data was generated using the same anti-PI 3 Kinase p85 alpha antibody clone [EPR18702] in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (cat# Anti-PI 3 Kinase p85 alpha antibody [EPR18702] ab191606).
Lane 1: Wild type HAP1 whole cell lysate (20 μg)
Lane 2: PIK3R1 knockout HAP1 whole cell lysate (20 μg)
Lane 3: Jurkat whole cell lysate (20 μg)Lanes 1 - 4: Merged signal (red and green). Green - Anti-PI 3 Kinase p85 alpha antibody [EPR18702] ab191606 observed at 90 kDa. Red - loading control, Anti-GAPDH antibody [mAbcam 9484] - Loading Control ab9484, observed at 37 kDa.
Anti-PI 3 Kinase p85 alpha antibody [EPR18702] ab191606 was shown to specifically react with PIK3R1 when PIK3R1 knockout samples were used. Wild-type and PIK3R1 knockout samples were subjected to SDS-PAGE. Anti-PI 3 Kinase p85 alpha antibody [EPR18702] ab191606 and Anti-GAPDH antibody [mAbcam 9484] - Loading Control ab9484 (Mouse anti GAPDH loading control) were incubated overnight at 4°C at 1000 dilution and 1/20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776 secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.
All lanes: Western blot - Anti-PI 3 Kinase p85 alpha antibody [EPR18702] (Anti-PI 3 Kinase p85 alpha antibody [EPR18702] ab191606)
Predicted band size: 83 kDa
![Immunocytochemistry/ Immunofluorescence - Anti-PI 3 Kinase p85 alpha antibody [EPR18702] - BSA and Azide free (ab223792), expandable thumbnail](https://content.abcam.com/products/images/pi-3-kinase-p85-alpha-antibody-epr18702-bsa-and-azide-free-ab223792--immunocytochemistry-immunofluorescence-img66131.jpg "Immunocytochemistry/ Immunofluorescence - Anti-PI 3 Kinase p85 alpha antibody [EPR18702] - BSA and Azide free (ab223792)")
Immunocytochemistry/ Immunofluorescence - Anti-PI 3 Kinase p85 alpha antibody [EPR18702] - BSA and Azide free (ab223792)
Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HepG2 (Human liver hepatocellular carcinoma cell line) cells labeling PI3K p85 with Anti-PI 3 Kinase p85 alpha antibody [EPR18702] ab191606 at 1/250 dilution, followed by Goat Anti-Rabbit IgG (Alexa Fluor® 488) (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) secondary antibody at 1/1000 dilution (green). Confocal image showing cytoplasmic staining on HepG2 cell line. The nuclear counter stain is DAPI (blue). Tubulin is detected with Anti-alpha Tubulin antibody - Loading Control (Anti-alpha Tubulin antibody [DM1A] - Loading Control ab7291) at 1/1000 dilution and Goat Anti-Mouse IgG (AlexaFluor®594) preadsorbed (Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) preadsorbed ab150120) at 1/1000 dilution (red).
The negative controls are as follows:
-ve control 1: Anti-PI 3 Kinase p85 alpha antibody [EPR18702] ab191606 at 1/500 dilution followed by Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) preadsorbed ab150120 at 1/1000 dilution.
-ve control 2: Anti-alpha Tubulin antibody [DM1A] - Loading Control ab7291 at 1/1000 dilution followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077 at 1/1000 dilution.This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-PI 3 Kinase p85 alpha antibody [EPR18702] ab191606).
![Western blot - Anti-PI 3 Kinase p85 alpha antibody [EPR18702] - BSA and Azide free (ab223792), expandable thumbnail](https://content.abcam.com/products/images/pi-3-kinase-p85-alpha-antibody-epr18702-bsa-and-azide-free-ab223792--western-blot-img54820.jpg "Western blot - Anti-PI 3 Kinase p85 alpha antibody [EPR18702] - BSA and Azide free (ab223792)")
Western blot - Anti-PI 3 Kinase p85 alpha antibody [EPR18702] - BSA and Azide free (ab223792)
This data was developed using the same antibody clone in a different buffer formulation (Anti-PI 3 Kinase p85 alpha antibody [EPR18702] ab191606).
Lanes 1- 2: Merged signal (red and green). Green - Anti-PI 3 Kinase p85 alpha antibody [EPR18702] ab191606 observed at 90 kDa. Red - Anti-GAPDH antibody [6C5] - Loading Control (Anti-GAPDH antibody [6C5] - Loading Control ab8245) observed at 37 kDa.
Anti-PI 3 Kinase p85 alpha antibody [EPR18702] ab191606 was shown to react with PI 3 Kinase p85 alpha in wild-type HeLa cells in western blot. Loss of signal was observed when knockout cell line Human PIK3R1 (PI 3 Kinase p85 alpha) knockout HeLa cell line ab265116 (knockout cell lysate Human PIK3R1 (PI 3 Kinase p85 alpha) knockout HeLa cell lysate ab257029) was used. Wild-type HeLa and PIK3R1 knockout HeLa cell lysates were subjected to SDS-PAGE. Membrane was blocked for 1 hour at room temperature in 0.1% TBST with 3% non-fat dried milk. Anti-PI 3 Kinase p85 alpha antibody [EPR18702] ab191606 and Anti-GAPDH antibody [6C5] - Loading Control (Anti-GAPDH antibody [6C5] - Loading Control ab8245) overnight at 4°C at a 1 in 1000 dilution and a 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye®800CW) preadsorbed (Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773) and Goat anti-Mouse IgG H&L (IRDye®680RD) preadsorbed (Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
All lanes: Western blot - Anti-PI 3 Kinase p85 alpha antibody [EPR18702] (Anti-PI 3 Kinase p85 alpha antibody [EPR18702] ab191606) at 1/1000 dilution
Lane 1: Wild-type HeLa cell lysate at 20 µg
Lane 2: PIK3R1 knockout HeLa cell lysate at 20 µg
Performed under reducing conditions.
Predicted band size: 83 kDa
Observed band size: 90 kDa
![Immunocytochemistry/ Immunofluorescence - Anti-PI 3 Kinase p85 alpha antibody [EPR18702] - BSA and Azide free (ab223792), expandable thumbnail](https://content.abcam.com/products/images/pi-3-kinase-p85-alpha-antibody-epr18702-bsa-and-azide-free-ab223792--immunocytochemistry-immunofluorescence-img71196.jpg "Immunocytochemistry/ Immunofluorescence - Anti-PI 3 Kinase p85 alpha antibody [EPR18702] - BSA and Azide free (ab223792)")
Immunocytochemistry/ Immunofluorescence - Anti-PI 3 Kinase p85 alpha antibody [EPR18702] - BSA and Azide free (ab223792)
Immunofluorescent analysis of 4% paraformaldehyde-fixed, 100% Methanol permeabilized NIH/3T3 (Mouse embryonic fibroblast cell line) cells labeling PI3K p85 with Anti-PI 3 Kinase p85 alpha antibody [EPR18702] ab191606 at 1/250 dilution, followed by Goat anti-rabbit IgG (Alexa Fluor® 488) (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) secondary antibody at 1/1000 dilution (green). Confocal image showing cytoplasmic staining on NIH/3T3 cell line. The nuclear counter stain is DAPI (blue). Tubulin is detected with Anti-alpha Tubulin antibody - Loading Control (Anti-alpha Tubulin antibody [DM1A] - Loading Control ab7291) at 1/1000 dilution and Goat Anti-Mouse IgG (AlexaFluor®594) preadsorbed (Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) preadsorbed ab150120) at 1/1000 dilution (red).
The negative controls are as follows:
-ve control 1: Anti-PI 3 Kinase p85 alpha antibody [EPR18702] ab191606 at 1/500 dilution followed by Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) preadsorbed ab150120 at 1/1000 dilution.
-ve control 2: Anti-alpha Tubulin antibody [DM1A] - Loading Control ab7291 at 1/1000 dilution followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077 at 1/1000 dilution.This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-PI 3 Kinase p85 alpha antibody [EPR18702] ab191606).
![Flow Cytometry (Intracellular) - Anti-PI 3 Kinase p85 alpha antibody [EPR18702] - BSA and Azide free (ab223792), expandable thumbnail](https://content.abcam.com/products/images/pi-3-kinase-p85-alpha-antibody-epr18702-bsa-and-azide-free-ab223792--flow-cytometry-intracellular-img26647.jpg "Flow Cytometry (Intracellular) - Anti-PI 3 Kinase p85 alpha antibody [EPR18702] - BSA and Azide free (ab223792)")
Flow Cytometry (Intracellular) - Anti-PI 3 Kinase p85 alpha antibody [EPR18702] - BSA and Azide free (ab223792)
Intracellular flow cytometric analysis of 4% paraformaldehyde-fixed NIH/3T3 (Mouse embryonic fibroblast cell line) cells labeling PI3K p85 with Anti-PI 3 Kinase p85 alpha antibody [EPR18702] ab191606 at 1/150 dilution (red) compared with a Rabbit IgG,monoclonal - Isotype control (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) (black) and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (blue). Goat anti Rabbit IgG (Alexa Fluor® 488) at 1/500 dilution was used as the secondary antibody.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-PI 3 Kinase p85 alpha antibody [EPR18702] ab191606).
![Immunoprecipitation - Anti-PI 3 Kinase p85 alpha antibody [EPR18702] - BSA and Azide free (ab223792), expandable thumbnail](https://content.abcam.com/products/images/pi-3-kinase-p85-alpha-antibody-epr18702-bsa-and-azide-free-ab223792--immunoprecipitation-img2095.jpg "Immunoprecipitation - Anti-PI 3 Kinase p85 alpha antibody [EPR18702] - BSA and Azide free (ab223792)")
Immunoprecipitation - Anti-PI 3 Kinase p85 alpha antibody [EPR18702] - BSA and Azide free (ab223792)
PI3K p85 was immunoprecipitated from 1mg of MCF7 (Human breast adenocarcinoma cell line) whole cell lysate with Anti-PI 3 Kinase p85 alpha antibody [EPR18702] ab191606 at 1/50 dilution. Western blot was performed from the immunoprecipitate using Anti-PI 3 Kinase p85 alpha antibody [EPR18702] ab191606 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (VeriBlot for IP Detection Reagent (HRP) ab131366), was used for detection at 1/10000 dilution.
Lane 1: MCF7 whole cell lysate, 10μg (Input).
Lane 2: Anti-PI 3 Kinase p85 alpha antibody [EPR18702] ab191606 IP in MCF7 whole cell lysate.
Lane 3: Rabbit IgG, monoclonal - Isotype Control (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of Anti-PI 3 Kinase p85 alpha antibody [EPR18702] ab191606 in MCF7 whole cell lysate.
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 5 seconds.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-PI 3 Kinase p85 alpha antibody [EPR18702] ab191606).
All lanes: Immunoprecipitation - Anti-PI 3 Kinase p85 alpha antibody [EPR18702] (Anti-PI 3 Kinase p85 alpha antibody [EPR18702] ab191606)
Predicted band size: 83 kDa
![Flow Cytometry (Intracellular) - Anti-PI 3 Kinase p85 alpha antibody [EPR18702] - BSA and Azide free (ab223792), expandable thumbnail](https://content.abcam.com/products/images/pi-3-kinase-p85-alpha-antibody-epr18702-bsa-and-azide-free-ab223792--flow-cytometry-intracellular-img68501.jpg "Flow Cytometry (Intracellular) - Anti-PI 3 Kinase p85 alpha antibody [EPR18702] - BSA and Azide free (ab223792)")
Flow Cytometry (Intracellular) - Anti-PI 3 Kinase p85 alpha antibody [EPR18702] - BSA and Azide free (ab223792)
Overlay histogram showing HepG2 cells fixed in 4% PFA and stained with Anti-PI 3 Kinase p85 alpha antibody [EPR18702] ab191606 at a dilution of 1/80 (red line). The secondary antibody used was Alexa Fluor® 488 goat anti-rabbit at a dilution of 1/500. Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) was used as an isotype control (black line) and cells incubated in the absence of both primary and secondary antibody were used as a negative control (blue line).
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-PI 3 Kinase p85 alpha antibody [EPR18702] ab191606).
![Immunoprecipitation - Anti-PI 3 Kinase p85 alpha antibody [EPR18702] - BSA and Azide free (ab223792), expandable thumbnail](https://content.abcam.com/products/images/pi-3-kinase-p85-alpha-antibody-epr18702-bsa-and-azide-free-ab223792--immunoprecipitation-img2095.jpg/_jcr_content/renditions/webp-475.webp "Immunoprecipitation - Anti-PI 3 Kinase p85 alpha antibody [EPR18702] - BSA and Azide free (ab223792)")
![Flow Cytometry (Intracellular) - Anti-PI 3 Kinase p85 alpha antibody [EPR18702] - BSA and Azide free (ab223792), expandable thumbnail](https://content.abcam.com/products/images/pi-3-kinase-p85-alpha-antibody-epr18702-bsa-and-azide-free-ab223792--flow-cytometry-intracellular-img68501.jpg/_jcr_content/renditions/webp-475.webp "Flow Cytometry (Intracellular) - Anti-PI 3 Kinase p85 alpha antibody [EPR18702] - BSA and Azide free (ab223792)")
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