Rabbit Polyclonal PI 3 Kinase p85 alpha antibody. Suitable for WB, IHC-P, ICC/IF and reacts with African green monkey, Human samples. Cited in 12 publications. Immunogen corresponding to Synthetic Peptide within Human PIK3R1.
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- Evidence-based complementary and alternative medic 2020:14035722020In Vitro Evaluation of the Neuroprotective Effect of Saponins by Activating the EGFR/PI3K/AKT Pathway.Applications:Unspecified applicationReactive species:Unspecified reactive speciesShuang Wu et. al.PubMed 32802113
- Evidence-based complementary and alternative medic 2020:45272832020Shenzhiling Oral Liquid Protects STZ-Injured Oligodendrocyte through PI3K/Akt-mTOR Pathway.Applications:Unspecified applicationReactive species:Unspecified reactive speciesZhenhong Liu et. al.PubMed 32774416
- Bioscience reports 40:2020Dynamic characterization of intestinal metaplasia in the gastric corpus mucosa of Atp4a-deficient mice.Applications:Unspecified applicationReactive species:Unspecified reactive speciesWei Liu,Liang-Jun Yang,Yuan-Liang Liu,Dong-Sheng Yuan,Zi-Ming Zhao,Qi Wang,Yan Yan,Hua-Feng PanPubMed 31904088
- Food & function 10:2161-21752019Inhibiting the PI3K/AKT/NF-κB signal pathway with nobiletin for attenuating the development of osteoarthritis: in vitro and in vivo studies.Applications:Unspecified applicationReactive species:Unspecified reactive speciesLinzhen Xie,Huanguang Xie,Chunhui Chen,Zhenyu Tao,Chuanxu Zhang,Leyi CaiPubMed 30938722
- Rejuvenation research 22:218-2292018Aloe-Emodin Ameliorates Renal Fibrosis Via Inhibiting PI3K/Akt/mTOR Signaling Pathway and .Applications:Unspecified applicationReactive species:Unspecified reactive speciesFang Dou,YueTong Liu,Limin Liu,Jingwen Wang,Ting Sun,Fei Mu,Qiyan Guo,Chao Guo,Na Jia,Wenxin Liu,Yi Ding,Aidong WenPubMed 30215298
- Molecular medicine reports 16:7706-77142017Edoxaban improves venous thrombosis via increasing hydrogen sulfide and homocysteine in rat model.Applications:Unspecified applicationReactive species:Unspecified reactive speciesWei Song et. al.PubMed 28944880
- International journal of immunopathology and pharm 30:25-432017Curcumin regulates insulin pathways and glucose metabolism in the brains of APPswe/PS1dE9 mice.Applications:Unspecified applicationReactive species:Unspecified reactive speciesPengwen Wang et. al.PubMed 28124574
- International journal of immunopathology and pharm 29:734-7412016Curcumin ameliorates insulin signalling pathway in brain of Alzheimer's disease transgenic mice.Applications:Unspecified applicationReactive species:Unspecified reactive speciesHui-Li Feng et. al.PubMed 27466310
- Biochemistry and biophysics reports 5:313-3272016Mangiferin attenuates oxidative stress induced renal cell damage through activation of PI3K induced Akt and Nrf-2 mediated signaling pathways.Applications:Unspecified applicationReactive species:Unspecified reactive speciesSukanya Saha et. al.PubMed 28955838
- British journal of cancer 110:1968-762014Clinical significance of p95HER2 overexpression, PTEN loss and PI3K expression in p185HER2-positive metastatic breast cancer patients treated with trastuzumab-based therapies.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.4
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Synthetic Peptide within Human PIK3R1. The exact immunogen used to generate this antibody is proprietary information. Database link P27986
Reactivity data
Select an application| WB | IHC-P | ICC/IF | |
|---|---|---|---|
| Human | Expected | Tested | Tested |
| African green monkey | Tested | Expected | Expected |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species African green monkey | Dilution info 1/500.00000 - 1/1000.00000 | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info Use at an assay dependent concentration. | Notes - |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/50.00000 - 1/100.00000 | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species African green monkey | Dilution info Use at an assay dependent concentration. | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/500 | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species African green monkey | Dilution info Use at an assay dependent concentration. | Notes - |
Target data
Function
Binds to activated (phosphorylated) protein-Tyr kinases, through its SH2 domain, and acts as an adapter, mediating the association of the p110 catalytic unit to the plasma membrane. Necessary for the insulin-stimulated increase in glucose uptake and glycogen synthesis in insulin-sensitive tissues. Plays an important role in signaling in response to FGFR1, FGFR2, FGFR3, FGFR4, KITLG/SCF, KIT, PDGFRA and PDGFRB. Likewise, plays a role in ITGB2 signaling (PubMed:17626883, PubMed:19805105, PubMed:7518429). Modulates the cellular response to ER stress by promoting nuclear translocation of XBP1 isoform 2 in a ER stress- and/or insulin-dependent manner during metabolic overloading in the liver and hence plays a role in glucose tolerance improvement (PubMed:20348923).
Additional Targets
PIK3R3
Alternative names
GRB1, PIK3R1, Phosphatidylinositol 3-kinase regulatory subunit alpha, PI3-kinase regulatory subunit alpha, PI3K regulatory subunit alpha, PtdIns-3-kinase regulatory subunit alpha, Phosphatidylinositol 3-kinase 85 kDa regulatory subunit alpha, PI3-kinase subunit p85-alpha, PtdIns-3-kinase regulatory subunit p85-alpha
Recommended products
Rabbit Polyclonal PI 3 Kinase p85 alpha antibody. Suitable for WB, IHC-P, ICC/IF and reacts with African green monkey, Human samples. Cited in 12 publications. Immunogen corresponding to Synthetic Peptide within Human PIK3R1.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.4
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Synthetic Peptide within Human PIK3R1. The exact immunogen used to generate this antibody is proprietary information. Database link P27986
- Purification technique
- Affinity purification Immunogen
- Specificity
PI3 kinase p85 alpha and gamma.
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Storage information
- Stable for 12 months at -20°C
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
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Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
The PI 3 Kinase p85 alpha + Gamma also known as PI3K is a regulatory subunit of the class IA phosphoinositide 3-kinases. PI3K is comprised of the p85 alpha (approximately 83 kDa) and p110 gamma subunits among others and plays an important role in cell signaling. The p85 alpha subunit is primarily expressed in immune cells liver and muscles while the p110 gamma is mostly found in leukocytes. This kinase is deeply involved in a variety of cellular processes like cell growth proliferation differentiation motility and survival.
- Biological function summary
The PI 3 Kinase p85 alpha + Gamma is an important regulator in signal transduction pathways and forms a heterodimer complex with p110 catalytic subunits. This kinase complex is activated by various growth factors and hormones through receptor tyrosine kinases. Upon activation PI3K phosphorylates phosphatidylinositol (45)-bisphosphate (PIP2) to produce phosphatidylinositol (345)-trisphosphate (PIP3) an essential second messenger that recruits downstream signaling proteins like AKT to the plasma membrane. This signal cascade regulates numerous cellular functions affecting homeostasis and adaptive responses.
- Pathways
The PI 3 Kinase p85 alpha + Gamma plays a significant role in the PI3K/AKT/mTOR and MAPK signaling pathways. These pathways are essential in controlling cell metabolism survival and growth. Within these cascades PI3K phosphorylates PIP2 to PIP3 creating a docking site for AKT which subsequently becomes activated and relays signals to downstream effectors including mTOR and GSK-3β. The proper functioning of these pathways is necessary for maintaining cellular equilibrium and their dysregulation might lead to pathological conditions.
- Associated diseases and disorders
Aberrant PI 3 Kinase p85 alpha + Gamma activity is implicated in cancer and immune disorders. In particular its malfunction is notable in various cancers including breast and prostate cancer where mutations can lead to constitutive activation of PI3K signaling promoting tumorigenic processes. Additionally mutations or altered expression of PI3K can contribute to immune-related conditions by affecting immune cell function and survival. Interactions between PI3K and other proteins such as PTEN a tumor suppressor represent key nodes in understanding these disease mechanisms and offer potential therapeutic targets.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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4 product images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PI 3 Kinase p85 alpha + Gamma antibody (ab74136)
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using ab74136, at 1/50 dilution, in the presence (right panel) or absence (left panel) of immunising peptide.
Immunocytochemistry/ Immunofluorescence - Anti-PI 3 Kinase p85 alpha + Gamma antibody (ab74136)
Immunofluorescence analysis of HeLa cells using ab74136, at 1/500 dilution, in the presence (right panel) or absence (left panel) of immunising peptide.
Western blot - Anti-PI 3 Kinase p85 alpha + Gamma antibody (ab74136)
All lanes: Western blot - Anti-PI 3 Kinase p85 alpha + Gamma antibody (ab74136) at 1/500 dilution
Lane 1: Extracts from COS7 cells, treated with H2O2 (100µM, 30mins) at 5 µg
Lane 2: Extracts from COS7 cells, treated with H2O2 (100µM, 30mins) at 5 µg with immunising peptide
Predicted band size: 84 kDa
Observed band size: 70 kDa, 84 kDa
Immunocytochemistry/ Immunofluorescence - Anti-PI 3 Kinase p85 alpha + Gamma antibody (ab74136)
ab74136 at 1/200 dilution staining PI 3 Kinase p85 alpha + Gamma in mouse embryonic stem cells by Immunocytochemistry/ Immunofluorescence. Cells were fixed in paraformaldehyde, permeabilized in 1:1 methanol, acetone and blocked in 1% serum for 30 minutes at 25°C. The primary antibody was used at 1/100 dilution in Goat block dillution buffer and incubated with sample at 25°C for 1 hours. An Alexa Fluor® 488 conjugated Goat polyclonal to rabbit IgG (H+L) was used as secondary at 1/500 dilution.
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