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AB278545

Anti- PI 3 Kinase p85 alpha (phospho Y467) + PI3 Kinase p55 (phospho Y199) antibody [PI3KY458-1A11]

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(1 Review)

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(152 Publications)

Anti-PI 3 Kinase p85 alpha (phospho Y467) + PI3 Kinase p55 (phospho Y199) antibody [PI3KY458-1A11] (ab278545) is a rabbit monoclonal antibody detecting PI 3 Kinase p85 alpha in Western Blot, Flow Cytometry. Suitable for Human, Mouse.

- Recombinant format for unrivalled batch-batch consistency
- Over 50 publications

View Alternative Names

GRB1, PIK3R1, Phosphatidylinositol 3-kinase regulatory subunit alpha, PI3-kinase regulatory subunit alpha, PI3K regulatory subunit alpha, PtdIns-3-kinase regulatory subunit alpha, Phosphatidylinositol 3-kinase 85 kDa regulatory subunit alpha, PI3-kinase subunit p85-alpha, PtdIns-3-kinase regulatory subunit p85-alpha

4 Images
Flow Cytometry - Anti- PI 3 Kinase p85 alpha (phospho Y467) + PI3 Kinase p55 (phospho Y199) antibody [PI3KY458-1A11] (AB278545)
  • Flow Cyt

Supplier Data

Flow Cytometry - Anti- PI 3 Kinase p85 alpha (phospho Y467) + PI3 Kinase p55 (phospho Y199) antibody [PI3KY458-1A11] (AB278545)

Flow cytometric analysis of Ramos (human Burkitt's lymphoma cell line) cells secondary antibody only negative control (blue) or untreated (red) or treated with pervanadate (green) using ab278545 at 0.1 µg/ml.

Flow Cytometry - Anti- PI 3 Kinase p85 alpha (phospho Y467) + PI3 Kinase p55 (phospho Y199) antibody [PI3KY458-1A11] (AB278545)
  • Flow Cyt

Supplier Data

Flow Cytometry - Anti- PI 3 Kinase p85 alpha (phospho Y467) + PI3 Kinase p55 (phospho Y199) antibody [PI3KY458-1A11] (AB278545)

Peptide blocking flow cytometric analysis of NIH/3T3 (mouse embryo fibroblast cell line) cells secondary antibody only negative control (light blue) or treated with imatinib (red) or with pervanadate (green) or imatinib and blocked with phospho-peptide (black) or pervanadate and blocked with phospho peptide (gold) or imatinib and blocked with non-phospho peptide (dark blue) or pervanadate and blocked with non-phospho peptide (purple) using ab278545 at 0.1 μg/ml.

Flow Cytometry - Anti- PI 3 Kinase p85 alpha (phospho Y467) + PI3 Kinase p55 (phospho Y199) antibody [PI3KY458-1A11] (AB278545)
  • Flow Cyt

Supplier Data

Flow Cytometry - Anti- PI 3 Kinase p85 alpha (phospho Y467) + PI3 Kinase p55 (phospho Y199) antibody [PI3KY458-1A11] (AB278545)

Flow cytometric analysis of NIH/3T3 (mouse embryo fibroblast cell line) cells secondary antibody only negative control (blue) or 0.1 µg/mL of isotype control (orange) or treated with imatinib (red) or with pervanadate (green) using ab278545 at 0.1 µg/ml.

Western blot - Anti- PI 3 Kinase p85 alpha (phospho Y467) + PI3 Kinase p55 (phospho Y199) antibody [PI3KY458-1A11] (AB278545)
  • WB

Supplier Data

Western blot - Anti- PI 3 Kinase p85 alpha (phospho Y467) + PI3 Kinase p55 (phospho Y199) antibody [PI3KY458-1A11] (AB278545)

Western blot analysis of NIH3T3 cell extract, untreated or treated with H2O2 with anti-Phospho-PI3Kinase p85 (Tyr458)/p55 (Tyr199) antibody ab278545. Loading control : AKT (Protein kinase B (PKB)).

All lanes:

Western blot - Anti- PI 3 Kinase p85 alpha (phospho Y467) + PI3 Kinase p55 (phospho Y199) antibody [PI3KY458-1A11] (ab278545) at 0.5 µg/mL

Lane 1:

Untreated NIH/3T3 (mouse embryo fibroblast cell line) cell extract

Lane 2:

H2O2-treated NIH/3T3 (mouse embryo fibroblast cell line) cell extract

Observed band size: 85 kDa,70 kDa

false

  • 660 APC

    APC Anti-PI3K p85 (phospho Y467) + PI3 Kinase p55 (phospho Y199) antibody [PI3KY458-1A11]

  • 519 FITC

    FITC Anti-PI 3 Kinase p85 alpha (phospho Y467) + PI3 Kinase p55 (phospho Y199) antibody [PI3KY458-1A11]

  • 578 PE

    PE Anti-PI 3 Kinase p85 alpha (phospho Y467) + PI3 Kinase p55 (phospho Y199) antibody [PI3KY458-1A11]

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

PI3KY458-1A11

Isotype

IgG

Light chain type

kappa

Carrier free

No

Reacts with

Mouse, Human

Applications

Flow Cyt, WB

applications

Immunogen

Synthetic Peptide within Human PIK3R1 phospho Y467. The exact immunogen used to generate this antibody is proprietary information.

P27986

Specificity

Recent data suggests this antibody can recognize PI 3 Kinase p85 and p55.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "0.1 µg/mL", "FlowCyt-species-notes": "<p></p>", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Mouse": { "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "0.1 µg/mL", "FlowCyt-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.5 µg/mL", "WB-species-notes": "<p></p>" } } }
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Product details

What is this antibody validated in?
Anti- PI 3 Kinase p85 alpha (phospho Y467) + PI3 Kinase p55 (phospho Y199) antibody [PI3KY458-1A11] (ab278545) is a rabbit recombinant monoclonal antibody and is validated for use in Western Blot (WB), Flow Cytometry (Flow Cyt) in Human, Mouse samples.

What is the molecular weight of PI 3 Kinase p85 alpha?
Anti- PI 3 Kinase p85 alpha (phospho Y467) + PI3 Kinase p55 (phospho Y199) [PI3KY458-1A11] (ab278545) specifically detects a band for PI 3 Kinase p85 alpha (UniProt: Q92569) at a molecular weight of 84kDa.

Trusted by the scientific community
Anti- PI 3 Kinase p85 alpha (phospho Y467) + PI3 Kinase p55 (phospho Y199) [PI3KY458-1A11] (ab278545) was first used in a scientific publication in 2021 and has been cited over 50 times in peer-reviewed journals.

Trial sizes available!
Test your antibody or perform pre-screening before committing to a larger quantity. Sold in 20µl. Discover our selection of trial-size antibodies.

Other related products
We have a range of other formats of antibody clone [PI3KY458-1A11] also available for your convenience: ab278545, PE - ab278691, FITC - ab278692, APC - ab278733

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A/G
Storage buffer
Preservative: 0.02% Sodium azide Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.1% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The PI 3 Kinase p85 alpha also known as PI3K p85 or PI3-kinase p85 is a regulatory subunit part of the phosphoinositide 3-kinases (PI3Ks) complex which is integral to cellular functions. The p85 alpha form partners with the catalytic subunit to form PI3K p55 known for its significant role in signaling. This subunit exhibits a molecular weight of approximately 85 kDa. It shows expression in various tissues including liver muscle and brain where it aids in cellular communication responding to extracellular signals.
Biological function summary

PI 3 Kinase p85 alpha plays an essential role in intracellular signaling where it functions as part of the PI3K complex. It functions to regulate metabolic processes by interacting with phosphatidylinositol lipids in the cell membrane. PI3K p55 form contributes to cell growth survival and proliferation often collaborating with the catalytic subunit p110 to execute these functions. The phospho PI3K form becomes active upon receiving external growth factors facilitating downstream signaling.

Pathways

The involvement of PI 3 Kinase p85 alpha extends to critical cellular pathways like the PI3K/AKT pathway and the insulin signaling pathway. It engages with proteins such as AKT (protein kinase B) and IRS-1 linking to various cellular functions including metabolism protein synthesis and cellular proliferation. The activity of PI3K p55 form regulates these pathways by influencing enzyme activity and pathways associated with cell survival and apoptosis.

Dysregulation of PI 3 Kinase p85 alpha associates closely with cancers and metabolic disorders like type 2 diabetes. Aberrations in the p55 form link to tumorigenesis due to its role in uncontrolled cell proliferation. PI3K pathway disorders also relate to insulin resistance where the malfunction of PI3K components disrupts normal glucose uptake and insulin signaling. This target when interacting with proteins like PTEN further influences the development of cancer highlighting its critical role in both oncogenic and metabolic pathologies.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Binds to activated (phosphorylated) protein-Tyr kinases, through its SH2 domain, and acts as an adapter, mediating the association of the p110 catalytic unit to the plasma membrane. Necessary for the insulin-stimulated increase in glucose uptake and glycogen synthesis in insulin-sensitive tissues. Plays an important role in signaling in response to FGFR1, FGFR2, FGFR3, FGFR4, KITLG/SCF, KIT, PDGFRA and PDGFRB. Likewise, plays a role in ITGB2 signaling (PubMed : 17626883, PubMed : 19805105, PubMed : 7518429). Modulates the cellular response to ER stress by promoting nuclear translocation of XBP1 isoform 2 in a ER stress- and/or insulin-dependent manner during metabolic overloading in the liver and hence plays a role in glucose tolerance improvement (PubMed : 20348923).
See full target information PIK3R1 phospho Y467
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Publications (152)

Recent publications for all applications. Explore the full list and refine your search

Physiological reports 13:e70553 PubMed40969143

2025

Midkine promotes PDGF-BB-induced proliferation, migration, and glycolysis of airway smooth muscle cells via the PI3K Akt pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Yongtian Xu,Wenli Li,Jun Shi,Yanfang Guo

Journal of clinical and translational hepatology 13:555-567 PubMed40937080

2025

CCNE1 Promotes the Progression of Hepatic Precancerous Lesion and the Malignant Phenotype of Hepatocellular Carcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Kai Zhang,Xue Hu,Lichao Yao,Wenzhi Guo

Journal of diabetes research 2025:9601959 PubMed40933728

2025

Myristoylated Cathelicidin-DM Fused With ANG1-7: A Novel Self-Assembling Antimicrobial Peptide for the Treatment and Mechanism of Diabetic Infected Wounds.

Applications

Unspecified application

Species

Unspecified reactive species

Rongqin Feng,Peng Wang,Li Fan,He Lu,Danna Yao,Panpan Sun,Zhonghua Liu,Fu Han,Xiaozhi Bai,Xuekang Yang,Juntao Han

European journal of histochemistry : EJH 69: PubMed40832995

2025

Aloe-emodin ameliorates chronic kidney disease fibrosis by inhibiting PI3K-mediated signaling pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Ming Chen,Wenhui Zhu,Yao Chen,Jingying Shang,Wenfan Wang,Xiaoming Yan,Peng Liu,Yabin Zhou

Scientific reports 15:29741 PubMed40804251

2025

HSF1 in macrophages suppressed the progression of asthma via modulating SIRPα/SHP2-Dectin-1/ SYK mediated ROS and inflammatory responses.

Applications

Unspecified application

Species

Unspecified reactive species

Xiaojuan Liu,Yingqian Zhang,Huifang Wu,Jingli Zhang,Yingxue Wang,Huifeng Zhang

Journal of orthopaedic surgery and research 20:745 PubMed40775331

2025

Yam polysaccharide promoted bone differentiation of MC3T3-E1 cells through targeted Inhibition of CASP3 activation.

Applications

Unspecified application

Species

Unspecified reactive species

Qiting Yu,LinHui Liu,TaiPing Jiang,Zhiyu Guan,ZhaoMing Liu

Bioactive materials 53:459-479 PubMed40747455

2025

Metformin carbon dots-based osteogenic and protein delivery system to promote bone regeneration in periodontitis.

Applications

Unspecified application

Species

Unspecified reactive species

Jingjing Wei,Kai Wang,Yongkai Li,Jiao Huang,Ping Deng,Xianbo Xia,Cong Yang,Ling Xu,Junji Xu

Scientific reports 15:27739 PubMed40730854

2025

CIAPIN1 promotes survival, proliferation, migration and glycolysis of endometrial cancer cells through PI3K/Akt pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Huanmei Sun,Juan Shao,Yingchun Duan,Jiashi Gu

Iranian journal of basic medical sciences 28:946-954 PubMed40703755

2025

Schisandrin B regulates the SIRT1/PI3K/Akt signaling pathway to ameliorate Ang II-infused cardiac fibrosis.

Applications

Unspecified application

Species

Unspecified reactive species

Xiaogang Zhang,Mengqing Shi,Zhongying Xing,Jie Su,Yuying Gu,Zhongping Ning

Chemical biology & drug design 105:e70135 PubMed40504801

2025

Anti-Osteoporosis Activity of Lycopene Through ESR1: Network Pharmacology, Molecular Docking, Imaging Technology, and Experimental Validation.

Applications

Unspecified application

Species

Unspecified reactive species

Benqian Zhao,Lulu Chen,Wei Wang,Weinan Xu,Bing Xu
View all publications
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