Anti-PI 3 Kinase p85 beta antibody [T15]

• WB

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Western blot - Anti-PI 3 Kinase p85 beta antibody [T15] (AB28356)

Lanes 1- 2 : Merged signal (red and green). Green - ab28356 observed at 85 kDa. Red - Anti-GAPDH antibody[EPR16891] - Loading Control (ab181602) observed at 37 kDa.

ab28356 was shown to react with PI 3 Kinase p85 beta in wild-type HEK-293T cells in western blot. Loss of signal was observed when knockout cell line ab266799 (knockout cell lysate ab257586) was used. Wild-type HEK-293T and PIK3R2 knockout HEK-293T cell lysates were subjected to SDS-PAGE. ab28356 and Anti-GAPDH antibody[EPR16891] - Loading Control (ab181602) overnight at 4°C at a 1 in 1000 dilution and a 1 in 20000 dilution respectively. Blots were developed with Goat anti-Mouse IgG H&L (IRDye^®800CW) preadsorbed (ab216772) and Goat Anti-Rabbit IgG H&L (IRDye^®680RD) preadsorbed (ab216777) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

All lanes:

Western blot - Anti-PI 3 Kinase p85 beta antibody [T15] (ab28356) at 1/1000 dilution

Lane 1:

Wild-type HEK-293T cell lysate at 20 µg

Lane 2:

PIK3R2 knockout HEK-293T cell lysate at 20 µg

Lane 2:

Western blot - Human PIK3R2 (PI 3 Kinase p85 beta) knockout HEK-293T cell line (<a href='/en-us/products/cell-lines/human-pik3r2-pi-3-kinase-p85-beta-knockout-hek-293t-cell-line-ab266799'>ab266799</a>)

Predicted band size: 81 kDa

Observed band size: 85 kDa

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• WB

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Western blot - Anti-PI 3 Kinase p85 beta antibody [T15] (AB28356)

Western Blotting of HEK293 and HepG2 cell lysates with Mouse anti Bovine PI-3 kinase p85 subunit beta (ab28356), recombinant p85ß is included as a positive control.

All lanes:

Western blot - Anti-PI 3 Kinase p85 beta antibody [T15] (ab28356)

Predicted band size: 81 kDa

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• WB

CiteAb

Western blot - Anti-PI 3 Kinase p85 beta antibody [T15] (AB28356)

PI 3 Kinase p85 beta western blot using anti-PI 3 Kinase p85 beta antibody [T15] ab28356. Publication image and figure legend from Du, C., Lv, Z., et al., 2014, J Transl Med, PubMed 25240815.

ab28356 was used in this publication in western blot. This may not be the same as the application(s) guaranteed by Abcam. For a full list of applications guaranteed by Abcam for ab28356 please see the product overview.

LRP6 and PIK3R2 are direct targets of miR-126-3p. (A) MiR-126-3p and its putative binding sequence in the 3'UTR of LRP6 and PIK3R2. The mutant miR-126-3p binding site was generated in the complementary site for the seed region of miR-126-3p. (B) Hep-G2 and BEL-7402 cells were co-transfected with miR-126-3p mimics or NC and wild-type or its mutant-type (either LRP6 or PIK3R2), and the luciferase activities were examined. The firefly luciferase activity of each sample was normalized to the Renilla luciferase activity. (C) Western blot results of endogenous LRP6, PIK3R2, beta-catenin and p-AKT proteins in HCC cells transfected with miR-126-3p mimics or anti-mir-126-3p and its NC. (D) Analysis of LRP6 and PIK3R2 expression in subcutaneous tumors by immunohistochemistry. NC indicated negative control, **p < 0.01.

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