AB138364

Anti-PI 3 Kinase p85 beta (phospho Y464) antibody

Name: Anti-PI 3 Kinase p85 beta (phospho Y464) antibody (ab138364) | Abcam
Brand: Abcam Limited
SKU: AB138364

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(43 Publications)

Rabbit Polyclonal PI 3 Kinase p85 beta phospho Y464 antibody. Suitable for WB and reacts with Human samples. Cited in 43 publications. Immunogen corresponding to Synthetic Peptide within Human PIK3R2 phospho Y464.

View Alternative Names

Phosphatidylinositol 3-kinase regulatory subunit beta, PI3-kinase regulatory subunit beta, PI3K regulatory subunit beta, PtdIns-3-kinase regulatory subunit beta, Phosphatidylinositol 3-kinase 85 kDa regulatory subunit beta, PI3-kinase subunit p85-beta, PtdIns-3-kinase regulatory subunit p85-beta, PIK3R2

1 Images

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Western blot - Anti-PI 3 Kinase p85 beta (phospho Y464) antibody (AB138364)

All lanes:

Western blot - Anti-PI 3 Kinase p85 beta (phospho Y464) antibody (ab138364) at 1/500 dilution

All lanes:

Jurkat cell extract at 30 µg

Predicted band size: 81 kDa

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Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

applications

Immunogen

Synthetic Peptide within Human PIK3R2 phospho Y464. The exact immunogen used to generate this antibody is proprietary information.

O00459

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Reactivity data

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Purification notes

ab138364 was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation.

Storage buffer

pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.88% Sodium chloride

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

PI 3-kinase p85 beta also known as PIK3R2 is a regulatory subunit of phosphoinositide 3-kinases (PI3K). This protein has a molecular weight of approximately 85 kilodaltons. It expresses widely in various tissues including brain liver and skeletal muscle. The p85 protein form interacts with the catalytic subunit p110 allowing for the proper localization and activation of PI3K in response to various extracellular signals.

Biological function summary

The regulatory subunit PI 3-kinase p85 beta is responsible for maintaining the balance of PI3K activity within the cellular environment. It forms a dynamic complex with the catalytic subunit influencing downstream signaling pathways. The presence of p85 beta ensures the stability and function of the PI3K complex essential for processes such as cell growth survival and metabolism.

Pathways

This protein plays a significant role in the PI3K/AKT/mTOR signaling pathway and also contributes to insulin signaling. It partners with p110 and mediates the transmission of signals from receptor tyrosine kinases (RTKs) like insulin receptors. The regulation by p85 beta affects protein synthesis and glucose uptake highlighting its function in cellular energy management.

Dysregulation of PI 3-kinase p85 beta associates frequently with types of cancer and type 2 diabetes mellitus. Aberrant signaling through the PI3K pathway involving interactions between p85 beta and p110 contributes to oncogenesis. Similarly inappropriate regulation of insulin signaling links p85 beta with insulin resistance an important factor in type 2 diabetes where it may interact with proteins such as IRS-1 (Insulin Receptor Substrate 1) to influence disease progression.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Regulatory subunit of phosphoinositide-3-kinase (PI3K), a kinase that phosphorylates PtdIns(4,5)P2 (Phosphatidylinositol 4,5-bisphosphate) to generate phosphatidylinositol 3,4,5-trisphosphate (PIP3). PIP3 plays a key role by recruiting PH domain-containing proteins to the membrane, including AKT1 and PDPK1, activating signaling cascades involved in cell growth, survival, proliferation, motility and morphology. Binds to activated (phosphorylated) protein-tyrosine kinases, through its SH2 domain, and acts as an adapter, mediating the association of the p110 catalytic unit to the plasma membrane. Indirectly regulates autophagy (PubMed : 23604317). Promotes nuclear translocation of XBP1 isoform 2 in a ER stress- and/or insulin-dependent manner during metabolic overloading in the liver and hence plays a role in glucose tolerance improvement (By similarity).

See full target information PIK3R2 phospho Y464

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Publications (43)

Recent publications for all applications. Explore the full list and refine your search

American journal of translational research 17:6103-6112 PubMed40950323

2025

TRAIL-mediated armA upregulation enhances the drug resistance of by activating the PI3K/AKT/mTOR signaling pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Bu Wang,Wei Zhang,Jianhua Liu,Hongxia Zhang,Maochen Li,Xiaocui Peng,Zhihua Zhang,Ning Song

Biochemical genetics : PubMed40736616

2025

MRPL15 as a Key Gene in the Malignant Proliferation of LUAD.

Applications

Unspecified application

Species

Unspecified reactive species

ZhuoCai Wang,YunFeng Qian,XuWen Lai,WenZhi Cui,JingXia Luo,WeiPeng Zeng,WenYuan He,Ming Liao

BMC cancer 25:804 PubMed40307753

2025

Comprehensive analysis illustrating the role of HOXB8 in head and neck squamous cell carcinoma: evidence from multi-omics analysis and experiments validation.

Applications

Unspecified application

Species

Unspecified reactive species

Jun-Wei Zhang,Xi-Lin Gao,Jing Wang,Xue-Li Fan,Qi-Wei Liang

Allergologia et immunopathologia 53:74-81 PubMed40088025

2025

Lysine hydroxylase 1 (PLOD1) regulates glucose metabolism and promotes Th17 cell differentiation in psoriasis.

Applications

Unspecified application

Species

Unspecified reactive species

Zhen Yue,Wanlu Zhang,Gege Zhu,Huiya Sun,Congjun Jiang

Stem cell research & therapy 16:70 PubMed39940043

2025

Pericyte-derived extracellular vesicles improve vascular barrier function in sepsis via the Angpt1/PI3K/AKT pathway and pericyte recruitment: an in vivo and in vitro study.

Applications

Unspecified application

Species

Unspecified reactive species

Zi-Sen Zhang,Ao Yang,Xi Luo,He-Nan Zhou,Yi-Yan Liu,Dai-Qin Bao,Jie Zhang,Jia-Tao Zang,Qing-Hui Li,Tao Li,Liang-Ming Liu

Drug development research 85:e70032 PubMed39670965

2024

AEBP1 Silencing Protects Against Cerebral Ischemia/Reperfusion Injury by Regulating Neuron Ferroptosis and Microglia M2 Polarization Through PRKCA-PI3K-Akt Axis.

Applications

Unspecified application

Species

Unspecified reactive species

Yafen Zhang,Yan Li,Fengli Liu

Histology and histopathology 40:1239-1251 PubMed39587911

2024

Hsa_circ_0088036 promotes tumorigenesis and chemotherapy resistance in hepatocellular carcinoma via the miR-140-3p/KIF2A axis.

Applications

Unspecified application

Species

Unspecified reactive species

Zhong Wang,Lei Wang,Guoqing Yin,Heng Li,Rong Zhang,Yuan Feng,Wen Chang

Acta histochemica 126:152190 PubMed39173233

2024

RGS1 targeted by miR-191-3p inhibited the stemness properties of esophageal cancer cells by suppressing CXCR4/PI3K/AKT signaling.

Applications

Unspecified application

Species

Unspecified reactive species

Jing Xun,Yuan Ma,Botao Wang,Xiaolin Jiang,Bin Liu,Ruifang Gao,Qiongli Zhai,Runfen Cheng,Xueliang Wu,Yu Wu,Qi Zhang

Open medicine (Warsaw, Poland) 19:20240991 PubMed39091610

2024

SHP-1 mediates cigarette smoke extract-induced epithelial-mesenchymal transformation and inflammation in 16HBE cells.

Applications

Unspecified application

Species

Unspecified reactive species

Quan He,Shuanglan Xu,Xiaomei Ma,Yuanxia Qian,Xuzhi Lu,Weiqi Feng,Zi Chen

Anais brasileiros de dermatologia 98:26-35 PubMed36272879

2022

Inhibition of ANGPT2 activates autophagy during hypertrophic scar formation via PI3K/AKT/mTOR pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Hongxin Chen,Kai Xu,Chao Sun,Si Gui,Juanjuan Wu,Song Wang

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

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Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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Anti-PI 3 Kinase p85 beta (phospho Y464) antibody

Western blot - Anti-PI 3 Kinase p85 beta (phospho Y464) antibody (AB138364)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Alternative Products

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Complementary Products

Primary Antibodies

Primary Antibodies

Secondary Antibodies

Primary Antibodies

Reactivity data

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (43)

Product promise