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Rabbit Recombinant Monoclonal PI3 Kinase p110 beta antibody. Suitable for WB, ICC/IF, Flow Cyt (Intra) and reacts with Rat, Mouse, Human samples. Cited in 103 publications.


Images

Western blot - Anti-PI3 Kinase p110 beta antibody [EPR5515(2)] (AB151549), expandable thumbnail
  • Immunocytochemistry/ Immunofluorescence - Anti-PI3 Kinase p110 beta antibody [EPR5515(2)] (AB151549), expandable thumbnail
  • Western blot - Anti-PI3 Kinase p110 beta antibody [EPR5515(2)] (AB151549), expandable thumbnail
  • Western blot - Anti-PI3 Kinase p110 beta antibody [EPR5515(2)] (AB151549), expandable thumbnail
  • Western blot - Anti-PI3 Kinase p110 beta antibody [EPR5515(2)] (AB151549), expandable thumbnail

Publications

  • Frontiers in oncology 13:11811762023
    immunotherapy combined with gemcitabine has a synergistic inhibitory effect on tumor growth and metastasis in murine Lewis lung cancer models.
    Applications:
    Unspecified application
    Reactive species:
    Unspecified reactive species
    Xiao Chen,Zhu Tao,Yun Liang,Meng Ma,Dickson Adah,Wenting Ding,Lili Chen,Xiaofen Li,Linglin Dai,Songwe Fanuel,Siting Zhao,Wen Hu,Donghai Wu,Ziyuan Duan,Fang Zhou,Li Qin,Xiaoping Chen,Zhaoqing Yang
    PubMed 37916167
  • Journal of cellular physiology 238:2827-28402023
    Mutual regulation between GDF11 and TET2 prevents senescence of mesenchymal stem cells.
    Applications:
    Unspecified application
    Reactive species:
    Unspecified reactive species
    Jiaming Gao,Hao Wang,Junyan Shen,Xiaojing Liu,Xiaoqi Zhu,Ce Huang,Gongchen Li,Yao Sun,Zhongmin Liu,Yi Eve Sun,Hailiang Liu
    PubMed 37801347

Key facts

Isotype

IgG

Host species

Rabbit

Storage buffer

Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Form

Liquid

Clonality

Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
WBICC/IFFlow Cyt (Intra)IHC-P
Human
Tested
Tested
Tested
Not recommended
Mouse
Tested
Expected
Expected
Not recommended
Rat
Tested
Expected
Expected
Not recommended

Tested
Tested

Species

Rat

Dilution info

1/1000

Notes

For unpurified use at 1/1000 - 1/10000.

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Species

Mouse

Dilution info

1/1000

Notes

For unpurified use at 1/1000 - 1/10000.

Species

Human

Dilution info

1/1000

Notes

For unpurified use at 1/1000 - 1/10000.

Tested
Tested

Species

Human

Dilution info

1/150

Notes

For unpurified use at 1/250 - 1/500.

Expected
Expected

Species

Rat, Mouse

Dilution info

Use at an assay dependent concentration.

Notes

-

Tested
Tested

Species

Human

Dilution info

1/20

Notes

For unpurified use at 1/100 - 1/1000. Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

Expected
Expected

Species

Rat, Mouse

Dilution info

Use at an assay dependent concentration.

Notes

-

Not recommended
Not recommended

Species

Human, Mouse, Rat

Dilution info

-

Notes

-

Associated Products

Select an associated product type

7 products for Alternative Product

    4 products for Alternative Version

      Target data

      Function

      Phosphoinositide-3-kinase (PI3K) phosphorylates phosphatidylinositol derivatives at position 3 of the inositol ring to produce 3-phosphoinositides (PubMed:15135396). Uses ATP and PtdIns(4,5)P2 (phosphatidylinositol 4,5-bisphosphate) to generate phosphatidylinositol 3,4,5-trisphosphate (PIP3) (PubMed:15135396). PIP3 plays a key role by recruiting PH domain-containing proteins to the membrane, including AKT1 and PDPK1, activating signaling cascades involved in cell growth, survival, proliferation, motility and morphology. Involved in the activation of AKT1 upon stimulation by G-protein coupled receptors (GPCRs) ligands such as CXCL12, sphingosine 1-phosphate, and lysophosphatidic acid. May also act downstream receptor tyrosine kinases. Required in different signaling pathways for stable platelet adhesion and aggregation. Plays a role in platelet activation signaling triggered by GPCRs, alpha-IIb/beta-3 integrins (ITGA2B/ ITGB3) and ITAM (immunoreceptor tyrosine-based activation motif)-bearing receptors such as GP6. Regulates the strength of adhesion of ITGA2B/ ITGB3 activated receptors necessary for the cellular transmission of contractile forces. Required for platelet aggregation induced by F2 (thrombin) and thromboxane A2 (TXA2). Has a role in cell survival. May have a role in cell migration. Involved in the early stage of autophagosome formation. Modulates the intracellular level of PtdIns3P (phosphatidylinositol 3-phosphate) and activates PIK3C3 kinase activity. May act as a scaffold, independently of its lipid kinase activity to positively regulate autophagy. May have a role in insulin signaling as scaffolding protein in which the lipid kinase activity is not required. May have a kinase-independent function in regulating cell proliferation and in clathrin-mediated endocytosis. Mediator of oncogenic signal in cell lines lacking PTEN. The lipid kinase activity is necessary for its role in oncogenic transformation. Required for the growth of ERBB2 and RAS driven tumors.

      Alternative names

      PI3-kinase subunit beta, PI3K-beta, PI3Kbeta, PtdIns-3-kinase subunit beta, PtdIns-3-kinase subunit p110-beta, p110beta, PIK3C1, PIK3CB

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      Rabbit Recombinant Monoclonal PI3 Kinase p110 beta antibody. Suitable for WB, ICC/IF, Flow Cyt (Intra) and reacts with Rat, Mouse, Human samples. Cited in 103 publications.

      Alternative names

      PI3-kinase subunit beta, PI3K-beta, PI3Kbeta, PtdIns-3-kinase subunit beta, PtdIns-3-kinase subunit p110-beta, p110beta, PIK3C1, PIK3CB

      Key facts

      Isotype

      IgG

      Form

      Liquid

      Clonality

      Monoclonal

      Immunogen
      • The exact immunogen used to generate this antibody is proprietary information.
      Clone number

      EPR5515(2)

      Purification technique

      Affinity purification Protein A

      Concentration
      Loading...

      Storage

      Shipped at conditions

      Blue Ice

      Appropriate long-term storage conditions

      -20°C

      Notes

      Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

      This product is a recombinant monoclonal antibody, which offers several advantages including:

      • - High batch-to-batch consistency and reproducibility
      • - Improved sensitivity and specificity
      • - Long-term security of supply
      • - Animal-free batch production

      For more information, read more on recombinant antibodies.

      Product promise

      We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

      In the unlikely event of one of our products not working as expected, you are covered by our product promise.

      Full details and terms and conditions can be found here:
      Terms & Conditions.

      7 product images

      • Western blot - Anti-PI3 Kinase p110 beta antibody [EPR5515(2)] (ab151549), expandable thumbnail

        Western blot - Anti-PI3 Kinase p110 beta antibody [EPR5515(2)] (ab151549)

        All lanes: Western blot - Anti-PI3 Kinase p110 beta antibody [EPR5515(2)] (ab151549) at 1/1000 dilution

        Lane 1: Rat Brain Lysate at 10 µg

        Lane 2: Mouse Brain Lysate at 10 µg

        Secondary

        All lanes: Goat Anti-Rabbit IgG, (H+L), HRP- conjugated at 1/1000 dilution

        Predicted band size: 123 kDa

      • Immunocytochemistry/ Immunofluorescence - Anti-PI3 Kinase p110 beta antibody [EPR5515(2)] (ab151549), expandable thumbnail

        Immunocytochemistry/ Immunofluorescence - Anti-PI3 Kinase p110 beta antibody [EPR5515(2)] (ab151549)

        ab151549 staining PI3 Kinase p110 in the HeLa cell line by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with 4% Paraformaldehyde permeabilized with 0.1% Triton X-100. Samples were incubated with primary antibody (1/150).Goat Anti-Rabbit IgG H&L (Alexa Fluor® 555) preadsorbed ab150082 an Alexa Fluor®555-conjugated Goat anti-rabbit IgG(1/500) was used as the secondary antibody. Nuclei were counterstained with DAPI.

      • Western blot - Anti-PI3 Kinase p110 beta antibody [EPR5515(2)] (ab151549), expandable thumbnail

        Western blot - Anti-PI3 Kinase p110 beta antibody [EPR5515(2)] (ab151549)

        All lanes: Western blot - Anti-PI3 Kinase p110 beta antibody [EPR5515(2)] (ab151549) at 1/5000 dilution

        All lanes: K562 Cell Lysate at 10 µg

        Secondary

        All lanes: Goat Anti-Rabbit IgG, (H+L), HRP- conjugated at 1/1000 dilution

        Predicted band size: 123 kDa

        Observed band size: 110 kDa

      • Western blot - Anti-PI3 Kinase p110 beta antibody [EPR5515(2)] (ab151549), expandable thumbnail

        Western blot - Anti-PI3 Kinase p110 beta antibody [EPR5515(2)] (ab151549)

        All lanes: Western blot - Anti-PI3 Kinase p110 beta antibody [EPR5515(2)] (ab151549) at 1/1000 dilution

        Lane 1: 293 Cell Lysate at 10 µg

        Lane 2: Jurkat Cell Lysate at 10 µg

        Lane 3: MCF-7 Cell Lysate at 10 µg

        Secondary

        All lanes: Goat Anti-Rabbit IgG, (H+L), HRP- conjugated at 1/1000 dilution

        Predicted band size: 123 kDa

        Observed band size: 110 kDa

      • Western blot - Anti-PI3 Kinase p110 beta antibody [EPR5515(2)] (ab151549), expandable thumbnail

        Western blot - Anti-PI3 Kinase p110 beta antibody [EPR5515(2)] (ab151549)

        All lanes: Western blot - Anti-PI3 Kinase p110 beta antibody [EPR5515(2)] (ab151549) at 1/1000 dilution

        Lane 1: MCF7 cell lysate at 10 µg

        Lane 2: Jurkat cell lysate at 10 µg

        Lane 3: K562 cell lysate at 10 µg

        Lane 4: 293T cell lysate at 10 µg

        Secondary

        All lanes: HRP labelled goat anti-rabbit at 1/2000 dilution

        Predicted band size: 123 kDa

      • Immunocytochemistry/ Immunofluorescence - Anti-PI3 Kinase p110 beta antibody [EPR5515(2)] (ab151549), expandable thumbnail

        Immunocytochemistry/ Immunofluorescence - Anti-PI3 Kinase p110 beta antibody [EPR5515(2)] (ab151549)

        Immunofluorescent analysis of 293 cells labelling PI3 Kinase p110 beta with unpurified ab151549 at 1/10 dilution.

      • Flow Cytometry (Intracellular) - Anti-PI3 Kinase p110 beta antibody [EPR5515(2)] (ab151549), expandable thumbnail

        Flow Cytometry (Intracellular) - Anti-PI3 Kinase p110 beta antibody [EPR5515(2)] (ab151549)

        Flow cytometry analysis of 80% methanol-fixed 0.1% Tween-20-permeabilized MCF7 cells stained with ab151549 at 1/50 dilution (red) and secondary Goat anti-Rabbit IgG (Alexa Fluor® 647, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 647) preadsorbed ab150083) at 1/5000 dilution. The isotype control was Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) / Black and the control used was cells without incubation with primary antibody and secondary antibody / Blue. 

        This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab151549). 

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      Product protocols

      For this product, it's our understanding that no specific protocols are required. You can:

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