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AB151549

Anti-PI3 Kinase p110 beta antibody [EPR5515(2)]

5

(2 Reviews)

|

(103 Publications)

Rabbit Recombinant Monoclonal PI3 Kinase p110 beta antibody. Suitable for WB, ICC/IF, Flow Cyt (Intra) and reacts with Rat, Mouse, Human samples. Cited in 103 publications.

View Alternative Names

PIK3C1, PIK3CB, PI3-kinase subunit beta, PI3K-beta, PI3Kbeta, PtdIns-3-kinase subunit beta, Serine/threonine protein kinase PIK3CB, PtdIns-3-kinase subunit p110-beta, p110beta

7 Images
Flow Cytometry (Intracellular) - Anti-PI3 Kinase p110 beta antibody [EPR5515(2)] (AB151549)
  • Flow Cyt (Intra)

Supplier Data

Flow Cytometry (Intracellular) - Anti-PI3 Kinase p110 beta antibody [EPR5515(2)] (AB151549)

Flow cytometry analysis of 80% methanol-fixed 0.1% Tween-20-permeabilized MCF7 cells stained with ab151549 at 1/50 dilution (red) and secondary Goat anti-Rabbit IgG (Alexa Fluor® 647, ab150083) at 1/5000 dilution. The isotype control was Rabbit monoclonal IgG (ab172730) / Black and the control used was cells without incubation with primary antibody and secondary antibody / Blue.  This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab151549).

Western blot - Anti-PI3 Kinase p110 beta antibody [EPR5515(2)] (AB151549)
  • WB

Unknown

Western blot - Anti-PI3 Kinase p110 beta antibody [EPR5515(2)] (AB151549)

All lanes:

Western blot - Anti-PI3 Kinase p110 beta antibody [EPR5515(2)] (ab151549) at 1/1000 dilution

Lane 1:

MCF7 cell lysate at 10 µg

Lane 2:

Jurkat cell lysate at 10 µg

Lane 3:

K562 cell lysate at 10 µg

Lane 4:

293T cell lysate at 10 µg

Secondary

All lanes:

HRP labelled goat anti-rabbit at 1/2000 dilution

Predicted band size: 123 kDa

false

Western blot - Anti-PI3 Kinase p110 beta antibody [EPR5515(2)] (AB151549)
  • WB

Supplier Data

Western blot - Anti-PI3 Kinase p110 beta antibody [EPR5515(2)] (AB151549)

All lanes:

Western blot - Anti-PI3 Kinase p110 beta antibody [EPR5515(2)] (ab151549) at 1/1000 dilution

Lane 1:

293 Cell Lysate at 10 µg

Lane 2:

Jurkat Cell Lysate at 10 µg

Lane 3:

MCF-7 Cell Lysate at 10 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), HRP- conjugated at 1/1000 dilution

Predicted band size: 123 kDa

Observed band size: 110 kDa

false

Western blot - Anti-PI3 Kinase p110 beta antibody [EPR5515(2)] (AB151549)
  • WB

Supplier Data

Western blot - Anti-PI3 Kinase p110 beta antibody [EPR5515(2)] (AB151549)

All lanes:

Western blot - Anti-PI3 Kinase p110 beta antibody [EPR5515(2)] (ab151549) at 1/5000 dilution

All lanes:

K562 Cell Lysate at 10 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), HRP- conjugated at 1/1000 dilution

Predicted band size: 123 kDa

Observed band size: 110 kDa

false

Immunocytochemistry/ Immunofluorescence - Anti-PI3 Kinase p110 beta antibody [EPR5515(2)] (AB151549)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-PI3 Kinase p110 beta antibody [EPR5515(2)] (AB151549)

ab151549 staining PI3 Kinase p110 in the HeLa cell line by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with 4% Paraformaldehyde permeabilized with 0.1% Triton X-100. Samples were incubated with primary antibody (1/150).ab150082 an Alexa Fluor®555-conjugated Goat anti-rabbit IgG(1/500) was used as the secondary antibody. Nuclei were counterstained with DAPI.

Immunocytochemistry/ Immunofluorescence - Anti-PI3 Kinase p110 beta antibody [EPR5515(2)] (AB151549)
  • ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-PI3 Kinase p110 beta antibody [EPR5515(2)] (AB151549)

Immunofluorescent analysis of 293 cells labelling PI3 Kinase p110 beta with unpurified ab151549 at 1/10 dilution.

Western blot - Anti-PI3 Kinase p110 beta antibody [EPR5515(2)] (AB151549)
  • WB

Supplier Data

Western blot - Anti-PI3 Kinase p110 beta antibody [EPR5515(2)] (AB151549)

All lanes:

Western blot - Anti-PI3 Kinase p110 beta antibody [EPR5515(2)] (ab151549) at 1/1000 dilution

Lane 1:

Rat Brain Lysate at 10 µg

Lane 2:

Mouse Brain Lysate at 10 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), HRP- conjugated at 1/1000 dilution

Predicted band size: 123 kDa

false

  • 617 Alexa Fluor® 594

    Alexa Fluor® 594 Anti-PI3 Kinase p110 beta antibody [EPR5515(2)]

  • 665 Alexa Fluor® 647

    Alexa Fluor® 647 Anti-PI3 Kinase p110 beta antibody [EPR5515(2)]

  • Carrier free

    Anti-PI3 Kinase p110 beta antibody [EPR5515(2)] - BSA and Azide free

  • 519 Alexa Fluor® 488

    Alexa Fluor® 488 Anti-PI3 Kinase p110 beta antibody [EPR5515(2)]

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR5515(2)

Isotype

IgG

Carrier free

No

Reacts with

Mouse, Rat, Human

Applications

WB, Flow Cyt (Intra), ICC/IF

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "<p><strong>For unpurified use at 1/1000 - 1/10000.</strong></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/150", "ICCIF-species-notes": "<p><strong>For unpurified use at 1/250 - 1/500.</strong></p>", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "1/20", "FlowCytIntra-species-notes": "<p>For unpurified use at 1/100 - 1/1000. <a href='/en-us/products/primary-antibodies/rabbit-igg-monoclonal-epr25a-isotype-control-ab172730'>ab172730</a> - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.</p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "<p><strong>For unpurified use at 1/1000 - 1/10000.</strong></p>", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "guaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>" }, "Rat": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "<p><strong>For unpurified use at 1/1000 - 1/10000.</strong></p>", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "guaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>" } } }
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Product details

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
Preservative: 0.01% Sodium azide Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Phosphatidylinositol 3-kinase p110 beta (PI3 kinase p110β) also known as PI3Kβ is an enzyme that belongs to the class I PI3-kinases. It functions as a catalytic subunit in the PI3K complex. The PI3 kinase p110β has a molecular mass of approximately 122 kDa. This protein is expressed in various tissues with notable presence in heart and skeletal muscles. The enzyme is responsible for phosphorylating PIP2 to generate PIP3 an essential step in signaling pathways that regulate cell growth and survival.
Biological function summary

PI3 kinase p110β plays a role in the regulation of cellular processes such as proliferation migration and metabolism. It is a component of the PI3K complex comprised of regulatory and catalytic subunits which form functional homodimers or heterodimers. This complex is activated by various receptors including G-protein coupled receptors (GPCRs) and tyrosine kinase receptors. By converting PIP2 to PIP3 it aids in the recruitment and activation of downstream signaling proteins like AKT.

Pathways

PI3 kinase p110β is integral in the PI3K/AKT signaling pathway and also contributes to the regulation of the mTOR pathway. These pathways are critical for controlling cellular responses to growth signals and maintaining homeostasis. Association with proteins like PTEN which negatively regulates the PI3K/AKT pathway highlights its importance in maintaining cellular balance. PI3 kinase p110β also interacts with other PI3 proteins like p110α showing the intricate network of signaling it participates in.

PI3 kinase p110β has been implicated in cancer and cardiovascular diseases. Its overactivation leads to unchecked cellular proliferation contributing significantly to oncogenesis. Alterations in the PI3K/AKT/mTOR pathway are common in breast cancer highlighting its interaction with proteins such as ERBB2. In cardiovascular disorders PI3 kinase p110β influences heart development and function where it can impact the action of related proteins like PI3 kinase p110α. The study of PI3 kinase p110β in these diseases offers potential for targeted therapies and improved understanding of its pathological roles.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Phosphoinositide-3-kinase (PI3K) phosphorylates phosphatidylinositol derivatives at position 3 of the inositol ring to produce 3-phosphoinositides (PubMed : 15135396). Uses ATP and PtdIns(4,5)P2 (phosphatidylinositol 4,5-bisphosphate) to generate phosphatidylinositol 3,4,5-trisphosphate (PIP3) (PubMed : 15135396). PIP3 plays a key role by recruiting PH domain-containing proteins to the membrane, including AKT1 and PDPK1, activating signaling cascades involved in cell growth, survival, proliferation, motility and morphology. Involved in the activation of AKT1 upon stimulation by G-protein coupled receptors (GPCRs) ligands such as CXCL12, sphingosine 1-phosphate, and lysophosphatidic acid. May also act downstream receptor tyrosine kinases. Required in different signaling pathways for stable platelet adhesion and aggregation. Plays a role in platelet activation signaling triggered by GPCRs, alpha-IIb/beta-3 integrins (ITGA2B/ ITGB3) and ITAM (immunoreceptor tyrosine-based activation motif)-bearing receptors such as GP6. Regulates the strength of adhesion of ITGA2B/ ITGB3 activated receptors necessary for the cellular transmission of contractile forces. Required for platelet aggregation induced by F2 (thrombin) and thromboxane A2 (TXA2). Has a role in cell survival. May have a role in cell migration. Involved in the early stage of autophagosome formation. Modulates the intracellular level of PtdIns3P (phosphatidylinositol 3-phosphate) and activates PIK3C3 kinase activity. May act as a scaffold, independently of its lipid kinase activity to positively regulate autophagy. May have a role in insulin signaling as scaffolding protein in which the lipid kinase activity is not required. May have a kinase-independent function in regulating cell proliferation and in clathrin-mediated endocytosis. Mediator of oncogenic signal in cell lines lacking PTEN. The lipid kinase activity is necessary for its role in oncogenic transformation. Required for the growth of ERBB2 and RAS driven tumors. Has also a protein kinase activity showing autophosphorylation (PubMed : 12502714).
See full target information PIK3CB
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Publications (103)

Recent publications for all applications. Explore the full list and refine your search

Frontiers in oncology 13:1181176 PubMed37916167

2023

immunotherapy combined with gemcitabine has a synergistic inhibitory effect on tumor growth and metastasis in murine Lewis lung cancer models.

Applications

Unspecified application

Species

Unspecified reactive species

Xiao Chen,Zhu Tao,Yun Liang,Meng Ma,Dickson Adah,Wenting Ding,Lili Chen,Xiaofen Li,Linglin Dai,Songwe Fanuel,Siting Zhao,Wen Hu,Donghai Wu,Ziyuan Duan,Fang Zhou,Li Qin,Xiaoping Chen,Zhaoqing Yang

Journal of cellular physiology 238:2827-2840 PubMed37801347

2023

Mutual regulation between GDF11 and TET2 prevents senescence of mesenchymal stem cells.

Applications

Unspecified application

Species

Unspecified reactive species

Jiaming Gao,Hao Wang,Junyan Shen,Xiaojing Liu,Xiaoqi Zhu,Ce Huang,Gongchen Li,Yao Sun,Zhongmin Liu,Yi Eve Sun,Hailiang Liu

Scientific reports 13:14092 PubMed37640741

2023

Orosomucoid 1 promotes colorectal cancer progression and liver metastasis by affecting PI3K/AKT pathway and inducing macrophage M2 polarization.

Applications

Unspecified application

Species

Unspecified reactive species

Lei Yue,Xiaozhang Xu,Shipeng Dai,Fan Xu,Wenhu Zhao,Jian Gu,Xinzheng Dai,Xiaofeng Qian

iScience 26:107534 PubMed37670789

2023

Inhibition of FNDC1 suppresses gastric cancer progression by interfering with Gβγ-VEGFR2 complex formation.

Applications

Unspecified application

Species

Unspecified reactive species

Yao Lu,Panpan Huang,Xueliang Zeng,Wenyu Liu,Rui Zhao,Jing Li,Gaolu Cao,Yaqiong Hu,Qiuxiang Xiao,Meng Wu,Weicai Huang,Xuerui Tang,Xiaojian Liu,Hulai Wei

iScience 26:107025 PubMed37389179

2023

Resistin targets TAZ to promote osteogenic differentiation through PI3K/AKT/mTOR pathway.

Applications

Unspecified application

Species

Unspecified reactive species

JingJing Shang,Zhentang Yu,Chengwei Xiong,Junjie Zhang,Jinhong Gong,Changlin Yu,Yong Huang,Xindie Zhou

Pharmaceutics 14: PubMed36559282

2022

Anti-Tumor Effects of Engineered VNP20009-Abvec-Igκ-mPD-1 Strain in Melanoma Mice via Combining the Oncolytic Therapy and Immunotherapy.

Applications

Unspecified application

Species

Unspecified reactive species

De-Xi Zhou,Xiao-He Wang,Xuan Xu,Wen-Jie Chen,Jing Wei,Ting-Tao Chen,Hong Wei

Science advances 8:eabq8109 PubMed36417513

2022

Functional specialization of different PI3K isoforms for the control of neuronal architecture, synaptic plasticity, and cognition.

Applications

Unspecified application

Species

Unspecified reactive species

Carla Sánchez-Castillo,María I Cuartero,Alba Fernández-Rodrigo,Víctor Briz,Sergio López-García,Raquel Jiménez-Sánchez,Juan A López,Mariona Graupera,José A Esteban

Frontiers in pharmacology 13:999851 PubMed36438802

2022

3,3'-diindolylmethane inhibits LPS-induced human chondrocytes apoptosis and extracellular matrix degradation by activating PI3K-Akt-mTOR-mediated autophagy.

Applications

Unspecified application

Species

Unspecified reactive species

Hao Tang,Kunpeng Qin,Anquan Wang,Shuang Li,Sheng Fang,Weilu Gao,Ming Lu,Wei Huang,Hui Zhang,Zongsheng Yin

Cardiovascular drugs and therapy 38:237-252 PubMed36350487

2022

Calsyntenin-1 Promotes Doxorubicin-induced Dilated Cardiomyopathy in Rats.

Applications

Unspecified application

Species

Unspecified reactive species

Mingxiang Zhu,Yibing Chen,Liting Cheng,Xin Li,Yanying Shen,Ge Guo,Xiang Xu,Hanlu Li,Hao Yang,Chunlei Liu,Kunlun He

American journal of reproductive immunology (New York, N.Y. : 1989) 89:e13634 PubMed36327113

2022

Erbu Zhuyu decoction improves endometrial angiogenesis via uterine natural killer cells and the PI3K/Akt/eNOS pathway a mouse model of embryo implantation dysfunction.

Applications

Unspecified application

Species

Unspecified reactive species

Mengmeng Shen,Yanfeng Liu,Xiaona Ma,Qingwen Zhu
View all publications
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