AB232690

Anti-PI3 Kinase p110 beta antibody [EPR5515(2)] - BSA and Azide free

RabMAb
Recombinant
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(1 Publication)

Rabbit Recombinant Monoclonal PI3 Kinase p110 beta antibody. Carrier free. Suitable for WB, ICC/IF, Flow Cyt (Intra) and reacts with Mouse, Rat, Human samples. Cited in 1 publication.

View Alternative Names

PIK3C1, PIK3CB, PI3-kinase subunit beta, PI3K-beta, PI3Kbeta, PtdIns-3-kinase subunit beta, Serine/threonine protein kinase PIK3CB, PtdIns-3-kinase subunit p110-beta, p110beta

3 Images

Flow Cytometry (Intracellular) - Anti-PI3 Kinase p110 beta antibody [EPR5515(2)] - BSA and Azide free (AB232690)

Flow Cyt (Intra)

Supplier Data

Flow Cytometry (Intracellular) - Anti-PI3 Kinase p110 beta antibody [EPR5515(2)] - BSA and Azide free (AB232690)

Flow cytometry analysis of 80% methanol-fixed 0.1% Tween-20-permeabilized MCF7 cells stained with ab151549 at 1/50 dilution (red) and secondary Goat anti-Rabbit IgG (Alexa Fluor® 647, ab150083) at 1/5000 dilution. The isotype control was Rabbit monoclonal IgG (ab172730) / Black and the control used was cells without incubation with primary antibody and secondary antibody / Blue. This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab151549).

Immunocytochemistry/ Immunofluorescence - Anti-PI3 Kinase p110 beta antibody [EPR5515(2)] - BSA and Azide free (AB232690)

ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-PI3 Kinase p110 beta antibody [EPR5515(2)] - BSA and Azide free (AB232690)

Immunofluorescent analysis of 293 cells labelling PI3 Kinase p110 beta with unpurified ab151549 at 1/10 dilution.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab151549).

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-PI3 Kinase p110 beta antibody [EPR5515(2)] - BSA and Azide free (AB232690)

ab151549 staining PI3 Kinase p110 in the HeLa cell line by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with 4% Paraformaldehyde permeabilized with 0.1% Triton X-100. Samples were incubated with primary antibody (1/150).ab150082 an Alexa Fluor®555-conjugated Goat anti-rabbit IgG(1/500) was used as the secondary antibody. Nuclei were counterstained with DAPI.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab151549).

Unconjugated

Anti-PI3 Kinase p110 beta antibody [EPR5515(2)]
617 Alexa Fluor® 594

Alexa Fluor® 594 Anti-PI3 Kinase p110 beta antibody [EPR5515(2)]
665 Alexa Fluor® 647

Alexa Fluor® 647 Anti-PI3 Kinase p110 beta antibody [EPR5515(2)]
519 Alexa Fluor® 488

Alexa Fluor® 488 Anti-PI3 Kinase p110 beta antibody [EPR5515(2)]

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR5515(2)

Isotype

IgG

Carrier free

Yes

Reacts with

Rat, Human, Mouse

Applications

WB, ICC/IF, Flow Cyt (Intra)

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "<a href='/en-us/products/primary-antibodies/rabbit-igg-monoclonal-epr25a-isotype-control-low-endotoxin-azide-free-ab199376'>ab199376</a> - Rabbit monoclonal IgG (Low endotoxin, Azide free), is suitable for use as an isotype control with this antibody.", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Mouse": { "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": " Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "predicted", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Rat": { "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "predicted", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }

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Product details

ab232690 is the carrier-free version of ab151549.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar^® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar^® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Storage information

Do Not Freeze

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Phosphatidylinositol 3-kinase p110 beta (PI3 kinase p110β) also known as PI3Kβ is an enzyme that belongs to the class I PI3-kinases. It functions as a catalytic subunit in the PI3K complex. The PI3 kinase p110β has a molecular mass of approximately 122 kDa. This protein is expressed in various tissues with notable presence in heart and skeletal muscles. The enzyme is responsible for phosphorylating PIP2 to generate PIP3 an essential step in signaling pathways that regulate cell growth and survival.

Biological function summary

PI3 kinase p110β plays a role in the regulation of cellular processes such as proliferation migration and metabolism. It is a component of the PI3K complex comprised of regulatory and catalytic subunits which form functional homodimers or heterodimers. This complex is activated by various receptors including G-protein coupled receptors (GPCRs) and tyrosine kinase receptors. By converting PIP2 to PIP3 it aids in the recruitment and activation of downstream signaling proteins like AKT.

Pathways

PI3 kinase p110β is integral in the PI3K/AKT signaling pathway and also contributes to the regulation of the mTOR pathway. These pathways are critical for controlling cellular responses to growth signals and maintaining homeostasis. Association with proteins like PTEN which negatively regulates the PI3K/AKT pathway highlights its importance in maintaining cellular balance. PI3 kinase p110β also interacts with other PI3 proteins like p110α showing the intricate network of signaling it participates in.

PI3 kinase p110β has been implicated in cancer and cardiovascular diseases. Its overactivation leads to unchecked cellular proliferation contributing significantly to oncogenesis. Alterations in the PI3K/AKT/mTOR pathway are common in breast cancer highlighting its interaction with proteins such as ERBB2. In cardiovascular disorders PI3 kinase p110β influences heart development and function where it can impact the action of related proteins like PI3 kinase p110α. The study of PI3 kinase p110β in these diseases offers potential for targeted therapies and improved understanding of its pathological roles.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Phosphoinositide-3-kinase (PI3K) phosphorylates phosphatidylinositol derivatives at position 3 of the inositol ring to produce 3-phosphoinositides (PubMed : 15135396). Uses ATP and PtdIns(4,5)P2 (phosphatidylinositol 4,5-bisphosphate) to generate phosphatidylinositol 3,4,5-trisphosphate (PIP3) (PubMed : 15135396). PIP3 plays a key role by recruiting PH domain-containing proteins to the membrane, including AKT1 and PDPK1, activating signaling cascades involved in cell growth, survival, proliferation, motility and morphology. Involved in the activation of AKT1 upon stimulation by G-protein coupled receptors (GPCRs) ligands such as CXCL12, sphingosine 1-phosphate, and lysophosphatidic acid. May also act downstream receptor tyrosine kinases. Required in different signaling pathways for stable platelet adhesion and aggregation. Plays a role in platelet activation signaling triggered by GPCRs, alpha-IIb/beta-3 integrins (ITGA2B/ ITGB3) and ITAM (immunoreceptor tyrosine-based activation motif)-bearing receptors such as GP6. Regulates the strength of adhesion of ITGA2B/ ITGB3 activated receptors necessary for the cellular transmission of contractile forces. Required for platelet aggregation induced by F2 (thrombin) and thromboxane A2 (TXA2). Has a role in cell survival. May have a role in cell migration. Involved in the early stage of autophagosome formation. Modulates the intracellular level of PtdIns3P (phosphatidylinositol 3-phosphate) and activates PIK3C3 kinase activity. May act as a scaffold, independently of its lipid kinase activity to positively regulate autophagy. May have a role in insulin signaling as scaffolding protein in which the lipid kinase activity is not required. May have a kinase-independent function in regulating cell proliferation and in clathrin-mediated endocytosis. Mediator of oncogenic signal in cell lines lacking PTEN. The lipid kinase activity is necessary for its role in oncogenic transformation. Required for the growth of ERBB2 and RAS driven tumors. Has also a protein kinase activity showing autophosphorylation (PubMed : 12502714).

See full target information PIK3CB

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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Journal of biological engineering 13:60 PubMed31303891

2019

Long non-coding RNA PVT1 knockdown suppresses fibroblast-like synoviocyte inflammation and induces apoptosis in rheumatoid arthritis through demethylation of .

Applications

Unspecified application

Species

Unspecified reactive species

Chun-Wang Zhang,Xia Wu,Dan Liu,Wei Zhou,Wei Tan,Yu-Xuan Fang,Yu Zhang,Yan-Qing Liu,Guo-Qing Li

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-PI3 Kinase p110 beta antibody [EPR5515(2)] - BSA and Azide free

Flow Cytometry (Intracellular) - Anti-PI3 Kinase p110 beta antibody [EPR5515(2)] - BSA and Azide free (AB232690)

Immunocytochemistry/ Immunofluorescence - Anti-PI3 Kinase p110 beta antibody [EPR5515(2)] - BSA and Azide free (AB232690)

Immunocytochemistry/ Immunofluorescence - Anti-PI3 Kinase p110 beta antibody [EPR5515(2)] - BSA and Azide free (AB232690)

Related conjugates and formulations (4)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (1)

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