AB32569

Anti-PI3 Kinase p110 beta antibody [Y384]

Name: Anti-PI3 Kinase p110 beta antibody [Y384] (ab32569) | Abcam
Brand: Abcam Limited
SKU: AB32569
Price: 365 USD
Availability: InStock
Rating: 5 (1 reviews)

RabMAb
Recombinant
KO Validated
20ul selling size
What is this?

(1 Review)

(20 Publications)

Rabbit Recombinant Monoclonal PI3 Kinase p110 beta antibody. Suitable for IP, WB and reacts with Human samples. Cited in 20 publications.

View Alternative Names

PIK3C1, PIK3CB, PI3-kinase subunit beta, PI3K-beta, PI3Kbeta, PtdIns-3-kinase subunit beta, Serine/threonine protein kinase PIK3CB, PtdIns-3-kinase subunit p110-beta, p110beta

3 Images

Lab

Immunoprecipitation - Anti-PI3 Kinase p110 beta antibody [Y384] (AB32569)

PI3 Kinase p110 beta was immunoprecipitated from 0.35 mg Jurkat (Human T cell leukemia T lymphocyte) whole cell lysate 10 µg with ab32569 at 1/50 dilution (2µg). VeriBlot for IP Detection Reagent (HRP) (ab131366) was used at 1/5000 dilution.

Lane 1 : Jurkat (Human T cell leukemia T lymphocyte) whole cell lysate 10 µg

Lane 2 : ab32569 IP in Jurkat whole cell lysate

Lane 3 : Rabbit monoclonal IgG (ab172730) instead of ab32569 in Jurkat whole cell lysate

Blocking and dilution buffer and concentration : 5% NFDM/TBST.

All lanes:

Immunoprecipitation - Anti-PI3 Kinase p110 beta antibody [Y384] (ab32569)

Predicted band size: 123 kDa

Observed band size: 110 kDa

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Lab

Western blot - Anti-PI3 Kinase p110 beta antibody [Y384] (AB32569)

Lane 1 : Wild-type HAP1 cell lysate (40 μg)
Lane 2 : PI3 Kinase p110 beta knockout HAP1 cell lysate (40 μg)
Lane 3 : Jurkat cell lysate (40 μg)
Lane 4 : K562 cell lysate (40 μg)
Lanes 1 - 4 : Merged signal (red and green). Green - ab32569 observed at 125 kDa. Red - loading control, ab8245, observed at 37 kDa.

ab32569 was shown to recognize PI3 Kinase p110 beta when PI3 Kinase p110 beta knockout samples were used, along with additional cross-reactive bands. Wild-type and PI3 Kinase p110 beta knockout samples were subjected to SDS-PAGE. ab32569 and ab8245 (loading control to GAPDH) were diluted at 1/500 and 1/10,000 dilution respectively and incubated overnight at 4C. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye^® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye^® 680RD) preadsorbed (ab216776) secondary antibodies at 1/10,000 dilution for 1 hour at room temperature before imaging.

All lanes:

Western blot - Anti-PI3 Kinase p110 beta antibody [Y384] (ab32569)

Predicted band size: 123 kDa

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Western blot - Anti-PI3 Kinase p110 beta antibody [Y384] (AB32569)

All lanes:

Western blot - Anti-PI3 Kinase p110 beta antibody [Y384] (ab32569) at 1/500 dilution

All lanes:

Jurkat cell lysate

Predicted band size: 123 kDa

Observed band size: 110 kDa

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Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

Y384

Isotype

IgG

Carrier free

Reacts with

Human

Applications

IP, WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Specificity

ab32569 recognises PI3-Kinases (PI3-Ks).

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Reactivity data

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Product details

Species reactivity
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species.
Please contact us for more information.

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.05% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Phosphatidylinositol 3-kinase p110 beta (PI3 kinase p110β) also known as PI3Kβ is an enzyme that belongs to the class I PI3-kinases. It functions as a catalytic subunit in the PI3K complex. The PI3 kinase p110β has a molecular mass of approximately 122 kDa. This protein is expressed in various tissues with notable presence in heart and skeletal muscles. The enzyme is responsible for phosphorylating PIP2 to generate PIP3 an essential step in signaling pathways that regulate cell growth and survival.

Biological function summary

PI3 kinase p110β plays a role in the regulation of cellular processes such as proliferation migration and metabolism. It is a component of the PI3K complex comprised of regulatory and catalytic subunits which form functional homodimers or heterodimers. This complex is activated by various receptors including G-protein coupled receptors (GPCRs) and tyrosine kinase receptors. By converting PIP2 to PIP3 it aids in the recruitment and activation of downstream signaling proteins like AKT.

Pathways

PI3 kinase p110β is integral in the PI3K/AKT signaling pathway and also contributes to the regulation of the mTOR pathway. These pathways are critical for controlling cellular responses to growth signals and maintaining homeostasis. Association with proteins like PTEN which negatively regulates the PI3K/AKT pathway highlights its importance in maintaining cellular balance. PI3 kinase p110β also interacts with other PI3 proteins like p110α showing the intricate network of signaling it participates in.

PI3 kinase p110β has been implicated in cancer and cardiovascular diseases. Its overactivation leads to unchecked cellular proliferation contributing significantly to oncogenesis. Alterations in the PI3K/AKT/mTOR pathway are common in breast cancer highlighting its interaction with proteins such as ERBB2. In cardiovascular disorders PI3 kinase p110β influences heart development and function where it can impact the action of related proteins like PI3 kinase p110α. The study of PI3 kinase p110β in these diseases offers potential for targeted therapies and improved understanding of its pathological roles.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Phosphoinositide-3-kinase (PI3K) phosphorylates phosphatidylinositol derivatives at position 3 of the inositol ring to produce 3-phosphoinositides (PubMed : 15135396). Uses ATP and PtdIns(4,5)P2 (phosphatidylinositol 4,5-bisphosphate) to generate phosphatidylinositol 3,4,5-trisphosphate (PIP3) (PubMed : 15135396). PIP3 plays a key role by recruiting PH domain-containing proteins to the membrane, including AKT1 and PDPK1, activating signaling cascades involved in cell growth, survival, proliferation, motility and morphology. Involved in the activation of AKT1 upon stimulation by G-protein coupled receptors (GPCRs) ligands such as CXCL12, sphingosine 1-phosphate, and lysophosphatidic acid. May also act downstream receptor tyrosine kinases. Required in different signaling pathways for stable platelet adhesion and aggregation. Plays a role in platelet activation signaling triggered by GPCRs, alpha-IIb/beta-3 integrins (ITGA2B/ ITGB3) and ITAM (immunoreceptor tyrosine-based activation motif)-bearing receptors such as GP6. Regulates the strength of adhesion of ITGA2B/ ITGB3 activated receptors necessary for the cellular transmission of contractile forces. Required for platelet aggregation induced by F2 (thrombin) and thromboxane A2 (TXA2). Has a role in cell survival. May have a role in cell migration. Involved in the early stage of autophagosome formation. Modulates the intracellular level of PtdIns3P (phosphatidylinositol 3-phosphate) and activates PIK3C3 kinase activity. May act as a scaffold, independently of its lipid kinase activity to positively regulate autophagy. May have a role in insulin signaling as scaffolding protein in which the lipid kinase activity is not required. May have a kinase-independent function in regulating cell proliferation and in clathrin-mediated endocytosis. Mediator of oncogenic signal in cell lines lacking PTEN. The lipid kinase activity is necessary for its role in oncogenic transformation. Required for the growth of ERBB2 and RAS driven tumors. Has also a protein kinase activity showing autophosphorylation (PubMed : 12502714).

See full target information PIK3CB

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Publications (20)

Recent publications for all applications. Explore the full list and refine your search

Thoracic cancer 16:e15485 PubMed39552193

2024

USP8-mediated PTK7 promotes PIK3CB-related pathway to accelerate the malignant progression of non-small cell lung cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Wencui Kong,Xuegang Feng,Zongyang Yu,Xingfeng Qi,Zhongquan Zhao

Tissue engineering and regenerative medicine 19:1251-1266 PubMed36042130

2022

Hsp20 Promotes Endothelial Progenitor Cell Angiogenesis via Activation of PI3K/Akt Signaling Pathway under Hypoxia.

Applications

Unspecified application

Species

Unspecified reactive species

Zhiqi Han,Xuan He,Yuan Feng,Weidong Jiang,Nuo Zhou,Xuanping Huang

Cancers 13: PubMed34830882

2021

Effects of Aerobic Exercise Training on MyomiRs Expression in Cachectic and Non-Cachectic Cancer Mice.

Applications

Unspecified application

Species

Unspecified reactive species

João Lucas Penteado Gomes,Gabriel Cardial Tobias,Tiago Fernandes,André Casanova Silveira,Carlos Eduardo Negrão,Roger Chammas,Patrícia Chakur Brum,Edilamar Menezes Oliveira

Frontiers in cell and developmental biology 9:720925 PubMed34790657

2021

The RNA Methyltransferase METTL3 Promotes Endothelial Progenitor Cell Angiogenesis in Mandibular Distraction Osteogenesis via the PI3K/AKT Pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Weidong Jiang,Peiqi Zhu,Fangfang Huang,Zhenchen Zhao,Tao Zhang,Xiaoning An,Fengchun Liao,Lina Guo,Yan Liu,Nuo Zhou,Xuanping Huang

Scientific reports 11:15761 PubMed34344927

2021

The COL-4A1 polypeptide destroy endothelial cells through the TGF-β/PI3K/AKT pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Ting Li,Zhonghui Ling,Kaipeng Xie,Yixiao Wang,Zhijing Miao,Xiaohong Ji,Jingyun Li,Wenwen Hou,Qiuqin Tang,Xiaojie Yuan,Nan Li,Chanjuan Li,Hongjuan Ding

Oncology reports 44:1333-1342 PubMed32945504

2020

Ginsenoside Rg3 enhances the anticancer effect of 5‑FU in colon cancer cells via the PI3K/AKT pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Shunzhong Hong,Wenjie Cai,Zicheng Huang,Yubin Wang,Xifeng Mi,Yisen Huang,Zhijin Lin,Xiangbo Chen

Journal of immunology (Baltimore, Md. : 1950) 203:137-147 PubMed31092639

2019

Effects of PI3K Inhibition on Afucosylated Antibody-Driven FcγRIIIa Events and Phospho-S6 Activity in NK Cells.

Applications

Unspecified application

Species

Unspecified reactive species

Valentina Romeo,Sarah Gierke,Kyle A Edgar,Scot D Liu

Oncology letters 15:1829-1838 PubMed29399196

2018

Microarray pathway analysis indicated that mitogen-activated protein kinase/extracellular signal-regulated kinase and insulin growth factor 1 signaling pathways were inhibited by small interfering RNA against AT-rich interactive domain 1A in endometrial cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Ye Yang,Wei Bao,Zhengyu Sang,Yongbing Yang,Meng Lu,Xiaowei Xi

Medical science monitor : international medical jo 24:623-635 PubMed29384143

2018

Expression and Comparison of Cbl-b in Lung Squamous Cell Carcinoma and Adenocarcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Peng Li,Hongliang Liu,Zhiqiang Zhang,Xiaodong Lv,Huijuan Wang,Jie Ma,Zhiyong Ma,Xiujuan Qu,Yue-E Teng

Experimental and therapeutic medicine 14:1967-1974 PubMed28962111

2017

MicroRNA-138 suppresses cell proliferation in laryngeal squamous cell carcinoma via inhibiting EZH2 and PI3K/AKT signaling.

Applications

Unspecified application

Species

Unspecified reactive species

Fengzhi Si,Jie Sun,Chunli Wang

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-PI3 Kinase p110 beta antibody [Y384]

Immunoprecipitation - Anti-PI3 Kinase p110 beta antibody [Y384] (AB32569)

Western blot - Anti-PI3 Kinase p110 beta antibody [Y384] (AB32569)

Western blot - Anti-PI3 Kinase p110 beta antibody [Y384] (AB32569)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Specificity

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (20)

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