Rabbit Recombinant Monoclonal PI3 Kinase p110 beta antibody. Suitable for IP, WB and reacts with Human samples. Cited in 17 publications.
pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 50% Glycerol (glycerin, glycerine), 49% PBS, 0.05% BSA
ICC/IF | ICC | IP | WB | Flow Cyt (Intra) | |
---|---|---|---|---|---|
Human | Not recommended | Not recommended | Tested | Tested | Not recommended |
Cat | Not recommended | Not recommended | Predicted | Predicted | Not recommended |
Dog | Not recommended | Not recommended | Predicted | Predicted | Not recommended |
Guinea pig | Not recommended | Not recommended | Predicted | Predicted | Not recommended |
Pig | Not recommended | Not recommended | Predicted | Predicted | Not recommended |
Species | Dilution info | Notes |
---|---|---|
Species Human, Guinea pig, Cat, Dog, Pig | Dilution info - | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Human, Guinea pig, Cat, Dog, Pig | Dilution info - | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Human | Dilution info 1/50 | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Guinea pig, Cat, Dog, Pig | Dilution info - | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Human | Dilution info 1/500 | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Guinea pig, Cat, Dog, Pig | Dilution info - | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Human, Guinea pig, Cat, Dog, Pig | Dilution info - | Notes - |
Select an associated product type
Phosphoinositide-3-kinase (PI3K) phosphorylates phosphatidylinositol derivatives at position 3 of the inositol ring to produce 3-phosphoinositides (PubMed:15135396). Uses ATP and PtdIns(4,5)P2 (phosphatidylinositol 4,5-bisphosphate) to generate phosphatidylinositol 3,4,5-trisphosphate (PIP3) (PubMed:15135396). PIP3 plays a key role by recruiting PH domain-containing proteins to the membrane, including AKT1 and PDPK1, activating signaling cascades involved in cell growth, survival, proliferation, motility and morphology. Involved in the activation of AKT1 upon stimulation by G-protein coupled receptors (GPCRs) ligands such as CXCL12, sphingosine 1-phosphate, and lysophosphatidic acid. May also act downstream receptor tyrosine kinases. Required in different signaling pathways for stable platelet adhesion and aggregation. Plays a role in platelet activation signaling triggered by GPCRs, alpha-IIb/beta-3 integrins (ITGA2B/ ITGB3) and ITAM (immunoreceptor tyrosine-based activation motif)-bearing receptors such as GP6. Regulates the strength of adhesion of ITGA2B/ ITGB3 activated receptors necessary for the cellular transmission of contractile forces. Required for platelet aggregation induced by F2 (thrombin) and thromboxane A2 (TXA2). Has a role in cell survival. May have a role in cell migration. Involved in the early stage of autophagosome formation. Modulates the intracellular level of PtdIns3P (phosphatidylinositol 3-phosphate) and activates PIK3C3 kinase activity. May act as a scaffold, independently of its lipid kinase activity to positively regulate autophagy. May have a role in insulin signaling as scaffolding protein in which the lipid kinase activity is not required. May have a kinase-independent function in regulating cell proliferation and in clathrin-mediated endocytosis. Mediator of oncogenic signal in cell lines lacking PTEN. The lipid kinase activity is necessary for its role in oncogenic transformation. Required for the growth of ERBB2 and RAS driven tumors. Has also a protein kinase activity showing autophosphorylation (PubMed:12502714).
PIK3C1, PIK3CB, PI3-kinase subunit beta, PI3K-beta, PI3Kbeta, PtdIns-3-kinase subunit beta, Serine/threonine protein kinase PIK3CB, PtdIns-3-kinase subunit p110-beta, p110beta
Rabbit Recombinant Monoclonal PI3 Kinase p110 beta antibody. Suitable for IP, WB and reacts with Human samples. Cited in 17 publications.
pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 50% Glycerol (glycerin, glycerine), 49% PBS, 0.05% BSA
ab32569 recognises PI3-Kinases (PI3-Ks).
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
For more information, read more on recombinant antibodies.
Phosphatidylinositol 3-kinase p110 beta (PI3 kinase p110β) also known as PI3Kβ is an enzyme that belongs to the class I PI3-kinases. It functions as a catalytic subunit in the PI3K complex. The PI3 kinase p110β has a molecular mass of approximately 122 kDa. This protein is expressed in various tissues with notable presence in heart and skeletal muscles. The enzyme is responsible for phosphorylating PIP2 to generate PIP3 an essential step in signaling pathways that regulate cell growth and survival.
PI3 kinase p110β plays a role in the regulation of cellular processes such as proliferation migration and metabolism. It is a component of the PI3K complex comprised of regulatory and catalytic subunits which form functional homodimers or heterodimers. This complex is activated by various receptors including G-protein coupled receptors (GPCRs) and tyrosine kinase receptors. By converting PIP2 to PIP3 it aids in the recruitment and activation of downstream signaling proteins like AKT.
PI3 kinase p110β is integral in the PI3K/AKT signaling pathway and also contributes to the regulation of the mTOR pathway. These pathways are critical for controlling cellular responses to growth signals and maintaining homeostasis. Association with proteins like PTEN which negatively regulates the PI3K/AKT pathway highlights its importance in maintaining cellular balance. PI3 kinase p110β also interacts with other PI3 proteins like p110α showing the intricate network of signaling it participates in.
PI3 kinase p110β has been implicated in cancer and cardiovascular diseases. Its overactivation leads to unchecked cellular proliferation contributing significantly to oncogenesis. Alterations in the PI3K/AKT/mTOR pathway are common in breast cancer highlighting its interaction with proteins such as ERBB2. In cardiovascular disorders PI3 kinase p110β influences heart development and function where it can impact the action of related proteins like PI3 kinase p110α. The study of PI3 kinase p110β in these diseases offers potential for targeted therapies and improved understanding of its pathological roles.
We have tested this species and application combination and it works. It is covered by our product promise.
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
ab32569 was shown to recognize PI3 Kinase p110 beta when PI3 Kinase p110 beta knockout samples were used, along with additional cross-reactive bands. Wild-type and PI3 Kinase p110 beta knockout samples were subjected to SDS-PAGE. ab32569 and Anti-GAPDH antibody [6C5] - Loading Control ab8245 (loading control to GAPDH) were diluted at 1/500 and 1/10,000 dilution respectively and incubated overnight at 4C. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776) secondary antibodies at 1/10,000 dilution for 1 hour at room temperature before imaging.
All lanes: Western blot - Anti-PI3 Kinase p110 beta antibody [Y384] (ab32569)
Predicted band size: 123 kDa
PI3 Kinase p110 beta was immunoprecipitated from 0.35 mg Jurkat (Human T cell leukemia T lymphocyte) whole cell lysate 10 μg with ab32569 at 1/50 dilution (2μg). VeriBlot for IP Detection Reagent (HRP)(VeriBlot for IP Detection Reagent (HRP) ab131366) was used at 1/5000 dilution.
Lane 1: Jurkat (Human T cell leukemia T lymphocyte) whole cell lysate 10 μg
Lane 2: ab32569 IP in Jurkat whole cell lysate
Lane 3: Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of ab32569 in Jurkat whole cell lysate
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
All lanes: Immunoprecipitation - Anti-PI3 Kinase p110 beta antibody [Y384] (ab32569)
Predicted band size: 123 kDa
Observed band size: 110 kDa
All lanes: Western blot - Anti-PI3 Kinase p110 beta antibody [Y384] (ab32569) at 1/500 dilution
All lanes: Jurkat cell lysate
Predicted band size: 123 kDa
Observed band size: 110 kDa
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
For licensing inquiries, please contact partnerships@abcam.com