Mouse Monoclonal PI 3 Kinase Class 2A/Cpk antibody. Carrier free. Suitable for WB and reacts with Mouse, Rat samples.
Images
![Western blot - Anti-PI3-Kinase p170 antibody [17/PI3-Kinase p170] - BSA and Azide Free (AB307099), expandable thumbnail](https://content.abcam.com/products/images/pi3-kinase-p170-antibody-17-pi3-kinase-p170-bsa-and-azide-free-ab307099--western-blot-img404520.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-PI3-Kinase p170 antibody [17/PI3-Kinase p170] - BSA and Azide Free (AB307099)")
Key facts
- Isotype
- IgG1
- Host species
- Mouse
- Storage buffer
pH: 7.2 - 7.4
Constituents: 100% PBS- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| WB | IHC-P | ICC/IF | IP | |
|---|---|---|---|---|
| Human | Not recommended | Not recommended | Not recommended | Not recommended |
| Mouse | Tested | Not recommended | Not recommended | Not recommended |
| Rat | Tested | Not recommended | Not recommended | Not recommended |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info - | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
IHC-P
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Species Mouse | Dilution info - | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Species Rat | Dilution info - | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
ICC/IF
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Mouse, Rat | Dilution info - | Notes - |
IP
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat, Human | Dilution info - | Notes - |
Target data
Function
Generates phosphatidylinositol 3-phosphate (PtdIns3P) and phosphatidylinositol 3,4-bisphosphate (PtdIns(3,4)P2) that act as second messengers. Has a role in several intracellular trafficking events. Functions in insulin signaling and secretion. Required for translocation of the glucose transporter SLC2A4/GLUT4 to the plasma membrane and glucose uptake in response to insulin-mediated RHOQ activation. Regulates insulin secretion through two different mechanisms: involved in glucose-induced insulin secretion downstream of insulin receptor in a pathway that involves AKT1 activation and TBC1D4/AS160 phosphorylation, and participates in the late step of insulin granule exocytosis probably in insulin granule fusion. Synthesizes PtdIns3P in response to insulin signaling. Functions in clathrin-coated endocytic vesicle formation and distribution. Regulates dynamin-independent endocytosis, probably by recruiting EEA1 to internalizing vesicles. In neurosecretory cells synthesizes PtdIns3P on large dense core vesicles. Participates in calcium induced contraction of vascular smooth muscle by regulating myosin light chain (MLC) phosphorylation through a mechanism involving Rho kinase-dependent phosphorylation of the MLCP-regulatory subunit MYPT1. May play a role in the EGF signaling cascade. May be involved in mitosis and UV-induced damage response. Required for maintenance of normal renal structure and function by supporting normal podocyte function. Involved in the regulation of ciliogenesis and trafficking of ciliary components (By similarity).
Alternative names
Cpk, Pik3c2a, Phosphatidylinositol 4-phosphate 3-kinase C2 domain-containing subunit alpha, PI3K-C2-alpha, PtdIns-3-kinase C2 subunit alpha, Cpk-m, Phosphoinositide 3-kinase-C2-alpha, p170
Recommended products
Mouse Monoclonal PI 3 Kinase Class 2A/Cpk antibody. Carrier free. Suitable for WB and reacts with Mouse, Rat samples.
Key facts
- Isotype
- IgG1
- Host species
- Mouse
- Storage buffer
pH: 7.2 - 7.4
Constituents: 100% PBS- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Carrier free
- Yes
- Clone number
- 17/PI3-Kinase p170
- Purification technique
- Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
- Storage information
- Do Not Freeze
Notes
ab307099 is the carrier-free version of Anti-PI3-Kinase p170 antibody [17/PI3-Kinase p170] ab307098.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
PI 3 Kinase Class 2A also known as Cpk is a significant enzyme involved in the regulation of various cellular processes. The target weighing approximately 170 kDa acts as a lipid kinase that phosphorylates phosphatidylinositol converting PI to PI3P. Cpk expresses mainly in tissues like the liver muscle and heart where it contributes to cellular homeostasis and metabolic regulation.
- Biological function summary
This enzyme family modulates intracellular signaling cascades influencing processes such as cell growth survival and metabolism. Cpk forms part of a larger complex that interacts with adaptor proteins facilitating its recruitment to cellular membranes. This interaction is important for its signaling activity and function in maintaining cellular equilibrium.
- Pathways
PI 3 Kinase Class 2A plays an integral role in the insulin signaling pathway and the TOR signaling pathway. In the insulin pathway it collaborates with proteins like Akt to modulate glucose uptake and storage in cells. Similarly in the TOR pathway Cpk contributes to nutrient sensing and cell growth regulation interconnected with proteins such as mTOR affecting protein synthesis and autophagy.
- Associated diseases and disorders
Dysregulation of PI 3 Kinase Class 2A links to cancer and type 2 diabetes. Alterations in its activity may lead to abnormal cell proliferation and survival characteristics of cancerous transformations associated with proteins like PTEN. Meanwhile its role in glucose metabolism ties it to insulin resistance and diabetes pathogenesis where interactions with insulin receptor substrates become important for disease progression.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
1 product image
![Western blot - Anti-PI3-Kinase p170 antibody [17/PI3-Kinase p170] - BSA and Azide Free (ab307099), expandable thumbnail](https://content.abcam.com/products/images/pi3-kinase-p170-antibody-17-pi3-kinase-p170-bsa-and-azide-free-ab307099--western-blot-img404520.jpg "Western blot - Anti-PI3-Kinase p170 antibody [17/PI3-Kinase p170] - BSA and Azide Free (ab307099)")
Western blot - Anti-PI3-Kinase p170 antibody [17/PI3-Kinase p170] - BSA and Azide Free (ab307099)
This data was developed using Anti-PI3-Kinase p170 antibody [17/PI3-Kinase p170] ab307098, the same antibody clone in a different buffer formulation.
Blocking and diluting buffer and concentration: 5% NFDM/TBST.
Low expression:liver, brain (PMID: 9337861).
The expression molecular weight observed is consistent with what has been described in the literature (PMID: 8662856).
Exposure times:
Lane 1: 59 seconds
Lane 2-9: 3 minutesAll lanes: Western blot - Anti-PI3-Kinase p170 antibody [17/PI3-Kinase p170] (Anti-PI3-Kinase p170 antibody [17/PI3-Kinase p170] ab307098) at 1/1000 dilution
Lane 1: Mouse kidney tissue lysate
Lane 2: Mouse testis tissue lysate
Lane 3: Mouse pancreas tissue lysate
Lane 4: Mouse liver tissue lysate
Lane 5: Mouse brain tissue lysate
Lane 6: Rat testis tissue lysate
Lane 7: Rat brain tissue lysate
Lane 8: NIH/3T3 (mouse embryonic fibroblast), whole cell lysate
Lane 9: C6 (rat glial tumor glial cell), whole cell lysate
SecondaryAll lanes: Peroxidase-Conjugated Goat anti-Mouse IgG (H+L) (ZB-2305) at 1/10000 dilution
Observed band size: 210 kDa, 190 kDa
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