AB134756

Anti-PI4 kinase beta/PI4KB antibody

Name: Anti-PI4 kinase beta/PI4KB antibody (ab134756) | Abcam
Brand: Abcam Limited
SKU: AB134756
Price: 545 USD
Availability: InStock

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(2 Publications)

Rabbit Polyclonal PI4 kinase beta/PI4KB antibody. Suitable for IP, WB and reacts with Human samples. Cited in 2 publications.

View Alternative Names

PIK4CB, PI4KB, Phosphatidylinositol 4-kinase beta, PI4K-beta, PI4Kbeta, PtdIns 4-kinase beta, NPIK, PI4K92, PI4KIII

2 Images

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Immunoprecipitation - Anti-PI4 kinase beta/PI4KB antibody (AB134756)

Lane 1 : Detection of Human PI4 kinase beta/PI4KB by Immunoprecipitation from HeLa whole cell lysate (1 mg for IP, 20% of IP loaded), using ab134756 at 6 μg/mg lysate for IP.
Lane 2 : Immunoprecipitation from HeLa whole cell lysate (1 mg for IP, 20% of IP loaded), using an antibody which recognized a downstream epitope of PI4 kinase beta/PI4KB.
Lane 3 : Immunoprecipitation from HeLa whole cell lysate (1 mg for IP, 20% of IP loaded), using Control IgG.

Subsequent Western blot detection used ab134756 at 1 μg/ml.
Western Blot Gel 4-8%

Developed using the ECL technique with an exposure time of 3 seconds.

All lanes:

Immunoprecipitation - Anti-PI4 kinase beta/PI4KB antibody (ab134756)

Predicted band size: 91 kDa

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Unknown

Western blot - Anti-PI4 kinase beta/PI4KB antibody (AB134756)

Western Blot Gel 4-8%

All lanes:

Western blot - Anti-PI4 kinase beta/PI4KB antibody (ab134756) at 0.1 µg/mL

Lane 1:

Whole cell lysate from HeLa cells at 50 µg

Lane 2:

Whole cell lysate from HeLa cells at 15 µg

Lane 3:

Whole cell lysate from 293T cells at 50 µg

Lane 4:

Whole cell lysate from Jurkat cells at 50 µg

Predicted band size: 91 kDa

true

Exposure time: 10s

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

WB, IP

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "2-10 µg for 1000 µg chromatin", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/2000 - 1/10000", "WB-species-notes": "<p></p>" }, "Baboon": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Gorilla": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Orangutan": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rabbit": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rhesus monkey": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7 - 8 Preservative: 0.09% Sodium azide Constituents: 99% Tris citrate/phosphate

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

PI4 kinase beta also known as PI4KB is an important enzyme involved in lipid signaling. It catalyzes the phosphorylation of phosphatidylinositol (PI) to produce phosphatidylinositol 4-phosphate (PI4P) an essential lipid for various cellular processes. PI4KB has a molecular mass of approximately 92 kDa. This enzyme shows expression in various tissues including the brain liver and pancreas. In cells PI4KB predominantly localizes at the Golgi apparatus and some plasma membrane sites.

Biological function summary

The enzyme plays a role in maintaining proper intracellular membrane structure and function. It operates as part of a larger complex involving additional elements such as the small GTPase ARF1 and the scaffold protein ACBD3 which are necessary for its recruitment and activity at Golgi membranes. PI4KB facilitates vesicular trafficking and supplies substrates for the production of other phosphoinositides impacting diverse cellular events like cell growth and signal transduction.

Pathways

PI4KB is an important participant in the phosphoinositide signaling pathway. This pathway regulates membrane dynamics and trafficking influencing cell signaling and membrane identity. PI4KB interacts with proteins such as PI4K2A and PI4K2B in the synthesis of different phosphoinositides which are components of the signaling cascade that include PI3K/AKT pathway having implications in cell growth and survival.

PI4KB has associations with certain infectious diseases and neurological disorders. The enzyme is linked to hepatitis C virus (HCV) replication where it interacts with viral proteins like NS5A facilitating the formation of replication complexes. Furthermore PI4KB has links to neurodegenerative disorders like Alzheimer's disease possibly through its influence on vesicular trafficking and lipid metabolism. These associations highlight its importance in disease mechanisms and as a potential therapeutic target.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Phosphorylates phosphatidylinositol (PI) in the first committed step in the production of the second messenger inositol-1,4,5,-trisphosphate (PIP). May regulate Golgi disintegration/reorganization during mitosis, possibly via its phosphorylation. Involved in Golgi-to-plasma membrane trafficking (By similarity) (PubMed : 10559940, PubMed : 11277933, PubMed : 12749687, PubMed : 9405935). May play an important role in the inner ear development.. (Microbial infection) Plays an essential role in Aichi virus RNA replication (PubMed : 22124328, PubMed : 22258260, PubMed : 27989622). Recruited by ACBD3 at the viral replication sites (PubMed : 22124328, PubMed : 27989622).. (Microbial infection) Required for cellular spike-mediated entry of human coronavirus SARS-CoV.

See full target information PI4KB

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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Cell reports 29:2229-2242.e4 PubMed31747597

2019

PI4KB on Inclusion Bodies Formed by ER Membrane Remodeling Facilitates Replication of Human Parainfluenza Virus Type 3.

Applications

Unspecified application

Species

Unspecified reactive species

Zhifei Li,Dong Guo,Yali Qin,Mingzhou Chen

Histochemistry and cell biology 150:255-269 PubMed29951776

2018

Histone deacetylase 1 expression is inversely correlated with age in the short-lived fish Nothobranchius furzeri.

Applications

Unspecified application

Species

Unspecified reactive species

Gordin Zupkovitz,Sabine Lagger,David Martin,Marianne Steiner,Astrid Hagelkruys,Christian Seiser,Christian Schöfer,Oliver Pusch

View all publications

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Product promise

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Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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Anti-PI4 kinase beta/PI4KB antibody

Immunoprecipitation - Anti-PI4 kinase beta/PI4KB antibody (AB134756)

Western blot - Anti-PI4 kinase beta/PI4KB antibody (AB134756)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Alternative Products

Primary Antibodies

Complementary Products

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (2)

Product promise