AB109418

Anti-PI4 kinase beta/PI4KB antibody [EPR3758(2)]

RabMAb
Recombinant
What is this?

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(3 Publications)

Rabbit Recombinant Monoclonal PI4 kinase beta/PI4KB antibody. Suitable for WB and reacts with Human samples. Cited in 3 publications.

View Alternative Names

PIK4CB, PI4KB, Phosphatidylinositol 4-kinase beta, PI4K-beta, PI4Kbeta, PtdIns 4-kinase beta, NPIK, PI4K92, PI4KIII

1 Images

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Western blot - Anti-PI4 kinase beta/PI4KB antibody [EPR3758(2)] (AB109418)

All lanes:

Western blot - Anti-PI4 kinase beta/PI4KB antibody [EPR3758(2)] (ab109418) at 1/1000 dilution

Lane 1:

HeLa cell lysate at 10 µg

Lane 2:

SH-SY5Y cell lysate at 10 µg

Lane 3:

BxPC-3 cell lysate at 10 µg

Lane 4:

Jurkat cell lysate at 10 µg

Predicted band size: 91 kDa

false

Carrier free

Anti-PI4 kinase beta/PI4KB antibody [EPR3758(2)] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR3758(2)

Isotype

IgG

Carrier free

Reacts with

Human

Applications

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

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Product details

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Preservative: 0.05% Sodium azide Constituents: 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 0.1% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Storage information

Stable for 12 months at -20°C

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

PI4 kinase beta also known as PI4KB is an important enzyme involved in lipid signaling. It catalyzes the phosphorylation of phosphatidylinositol (PI) to produce phosphatidylinositol 4-phosphate (PI4P) an essential lipid for various cellular processes. PI4KB has a molecular mass of approximately 92 kDa. This enzyme shows expression in various tissues including the brain liver and pancreas. In cells PI4KB predominantly localizes at the Golgi apparatus and some plasma membrane sites.

Biological function summary

The enzyme plays a role in maintaining proper intracellular membrane structure and function. It operates as part of a larger complex involving additional elements such as the small GTPase ARF1 and the scaffold protein ACBD3 which are necessary for its recruitment and activity at Golgi membranes. PI4KB facilitates vesicular trafficking and supplies substrates for the production of other phosphoinositides impacting diverse cellular events like cell growth and signal transduction.

Pathways

PI4KB is an important participant in the phosphoinositide signaling pathway. This pathway regulates membrane dynamics and trafficking influencing cell signaling and membrane identity. PI4KB interacts with proteins such as PI4K2A and PI4K2B in the synthesis of different phosphoinositides which are components of the signaling cascade that include PI3K/AKT pathway having implications in cell growth and survival.

PI4KB has associations with certain infectious diseases and neurological disorders. The enzyme is linked to hepatitis C virus (HCV) replication where it interacts with viral proteins like NS5A facilitating the formation of replication complexes. Furthermore PI4KB has links to neurodegenerative disorders like Alzheimer's disease possibly through its influence on vesicular trafficking and lipid metabolism. These associations highlight its importance in disease mechanisms and as a potential therapeutic target.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Phosphorylates phosphatidylinositol (PI) in the first committed step in the production of the second messenger inositol-1,4,5,-trisphosphate (PIP). May regulate Golgi disintegration/reorganization during mitosis, possibly via its phosphorylation. Involved in Golgi-to-plasma membrane trafficking (By similarity) (PubMed : 10559940, PubMed : 11277933, PubMed : 12749687, PubMed : 9405935). May play an important role in the inner ear development.. (Microbial infection) Plays an essential role in Aichi virus RNA replication (PubMed : 22124328, PubMed : 22258260, PubMed : 27989622). Recruited by ACBD3 at the viral replication sites (PubMed : 22124328, PubMed : 27989622).. (Microbial infection) Required for cellular spike-mediated entry of human coronavirus SARS-CoV.

See full target information PI4KB

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Publications (3)

Recent publications for all applications. Explore the full list and refine your search

The EMBO journal 40:e105990 PubMed34019311

2021

NPC1 regulates the distribution of phosphatidylinositol 4-kinases at Golgi and lysosomal membranes.

Applications

Unspecified application

Species

Unspecified reactive species

Candice Kutchukian,Oscar Vivas,Maria Casas,Julia G Jones,Scott A Tiscione,Sergi Simó,Daniel S Ory,Rose E Dixon,Eamonn J Dickson

Molecular medicine reports 12:6738-44 PubMed26328522

2015

STAT3‑regulated long non‑coding RNAs lnc‑7SK and lnc‑IGF2‑AS promote hepatitis C virus replication.

Applications

Species

Unspecified reactive species

Yulin Xiong,Ming Jia,Jing Yuan,Changjiang Zhang,Yan Zhu,Xuemei Kuang,Lin Lan,Xiaohong Wang

Journal of virology 87:11031-46 PubMed23926333

2013

The Golgi protein ACBD3, an interactor for poliovirus protein 3A, modulates poliovirus replication.

Applications

Unspecified application

Species

Unspecified reactive species

François Téoulé,Cynthia Brisac,Isabelle Pelletier,Pierre-Olivier Vidalain,Sophie Jégouic,Carmen Mirabelli,Maël Bessaud,Nicolas Combelas,Arnaud Autret,Frédéric Tangy,Francis Delpeyroux,Bruno Blondel

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-PI4 kinase beta/PI4KB antibody [EPR3758(2)]

Western blot - Anti-PI4 kinase beta/PI4KB antibody [EPR3758(2)] (AB109418)

Related conjugates and formulations (1)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (3)

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Product promise