Rabbit Polyclonal PI4KA antibody. Suitable for WB, ICC/IF and reacts with Human samples. Cited in 2 publications. Immunogen corresponding to Recombinant Fragment Protein within Human PI4KA aa 1-350.
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Publications
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- International journal of biological sciences 18:4963-49832022Human promotes growth and metastasis via sphingolipid metabolite ceramide and PI4KA/AKT signaling in pancreatic cancer cells.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYanqun Zhang,Shenying Ji,Xiangyu Zhang,Mengyun Lu,Yihong Hu,Yucheng Han,Guanghou Shui,Sin Man Lam,Xianqiong ZouPubMed 35982909
- BioMed research international 2020:96925032020Phosphatidylinositol-4,5-Biphosphate (PI(4,5)P) Is Required for Rapid Endocytosis in Chromaffin Cells.Applications:Unspecified applicationReactive species:Unspecified reactive speciesFouad AziziPubMed 32964048
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7
Preservative: 0.025% Proclin 300
Constituents: 79% PBS, 20% Glycerol (glycerin, glycerine)- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Recombinant Fragment Protein within Human PI4KA aa 1-350. The exact immunogen used to generate this antibody is proprietary information. Database link P42356
Reactivity data
Select an application| WB | ICC/IF | |
|---|---|---|
| Human | Tested | Tested |
| Mouse | Predicted | Predicted |
| Rat | Predicted | Predicted |
| Cow | Predicted | Predicted |
| Xenopus laevis | Predicted | Predicted |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/500.00000 - 1/3000.00000 | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat, Cow, Xenopus laevis | Dilution info - | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/100.00000 - 1/500.00000 | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat, Cow, Xenopus laevis | Dilution info - | Notes - |
Target data
Function
Acts on phosphatidylinositol (PtdIns) in the first committed step in the production of the second messenger inositol-1,4,5,-trisphosphate.
Alternative names
PIK4, PIK4CA, PI4KA, Phosphatidylinositol 4-kinase alpha, PI4-kinase alpha, PI4K-alpha, PtdIns-4-kinase alpha, Phosphatidylinositol 4-Kinase III alpha
Recommended products
Rabbit Polyclonal PI4KA antibody. Suitable for WB, ICC/IF and reacts with Human samples. Cited in 2 publications. Immunogen corresponding to Recombinant Fragment Protein within Human PI4KA aa 1-350.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7
Preservative: 0.025% Proclin 300
Constituents: 79% PBS, 20% Glycerol (glycerin, glycerine)- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Recombinant Fragment Protein within Human PI4KA aa 1-350. The exact immunogen used to generate this antibody is proprietary information. Database link P42356
- Purification technique
- Affinity purification Immunogen
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.
Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
PI4KA also known as phosphatidylinositol 4-kinase alpha is an enzyme responsible for phosphorylating phosphatidylinositol to produce phosphatidylinositol 4-phosphate (PI4P). The protein has a mass of approximately 230 kDa. PI4KA is expressed broadly with significant levels in the brain heart and other tissues. It plays an important role as a regulator of cellular lipid metabolism and intracellular signaling.
- Biological function summary
PI4KA functions in the context of membrane dynamics and acts as part of a multi-subunit complex. This protein is important in maintaining the proper lipid composition of the Golgi apparatus and other organelles which is essential for vesicle trafficking. By generating PI4P PI4KA serves as an important mediator in the distribution and dynamics of cellular membranes aiding in various cellular processes including endocytosis and autophagy. Its activity impacts several downstream signaling pathways and cellular activities.
- Pathways
PI4KA is a significant contributor to the phosphoinositide signaling pathway which plays an essential role in regulating intracellular processes. It interacts with phosphatidylinositol-45-bisphosphate (PIP2) and phospholipase C supporting the synthesis of further derivatives that modulate calcium influx and other cellular responses. Additionally PI4KA is involved in the maintenance and function of the secretory pathway where it coordinates with proteins such as Osh1 and CERT influencing lipid exchange and protein trafficking.
- Associated diseases and disorders
PI4KA's dysfunction has been linked to neurological disorders and cancer. Alterations in its activity can contribute to conditions like neurodegenerative diseases due to disrupted membrane composition in neural tissues. Furthermore PI4KA's role in cancer is illuminated through its interaction with proteins like AKT where aberrant PI4P levels disrupt normal cell growth signals leading to oncogenesis. Understanding PI4KA's pathways and interactions is critical as they offer potential therapeutic targets for these diseases.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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2 product images
Western blot - Anti-PI4KA antibody (ab111565)
5% SDS-PAGE.
All lanes: Western blot - Anti-PI4KA antibody (ab111565) at 1/1000 dilution
All lanes: 293T whole cell lysate at 30 µg
Predicted band size: 123 kDa, 134 kDa, 231 kDa
Immunocytochemistry/ Immunofluorescence - Anti-PI4KA antibody (ab111565)
ab111565 at 1/200 dilution staining PI4KA in methanol-fixed A431 cells by Immunofluorescence. Lower image shows cells co-stained with Hoechst 33342.
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