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AB111565

Anti-PI4KA antibody

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(2 Publications)

Rabbit Polyclonal PI4KA antibody. Suitable for WB, ICC/IF, IHC-P and reacts with Human samples. Cited in 2 publications. Immunogen corresponding to Recombinant Fragment Protein within Human PI4KA aa 1-350.

View Alternative Names

PIK4, PIK4CA, PI4KA, Phosphatidylinositol 4-kinase alpha, PI4-kinase alpha, PI4K-alpha, PtdIns-4-kinase alpha, Phosphatidylinositol 4-Kinase III alpha

2 Images
Western blot - Anti-PI4KA antibody (AB111565)
  • WB

Supplier Data

Western blot - Anti-PI4KA antibody (AB111565)

Samples were separated by 5% SDS-PAGE.

All lanes:

Western blot - Anti-PI4KA antibody (ab111565) at 1/1000 dilution

Lane 1:

293T whole cell lysates at 30 µg

Lane 2:

A431 whole cell lysates at 30 µg

Lane 3:

HeLa whole cell lysates at 30 µg

Lane 4:

HepG2 whole cell lysates at 30 µg

Secondary

All lanes:

HRP conjugated anti rabbit IgG antibody

Predicted band size: 231 kDa

false

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PI4KA antibody (AB111565)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PI4KA antibody (AB111565)

Immunohistochemical analysis of paraffin embedded human gastric cancer tissue with ab111565 at a 1/500 dilution.

Antigen retrieval : Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

IHC-P, WB, ICC/IF

applications

Immunogen

Recombinant Fragment Protein within Human PI4KA aa 1-350. The exact immunogen used to generate this antibody is proprietary information.

P42356

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500.00000 - 1/3000.00000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100.00000 - 1/1000.00000", "ICCIF-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/1000", "IHCP-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Rat": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Cow": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Xenopus laevis": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7 Preservative: 0.025% Proclin 300 Constituents: 79% PBS, 20% Glycerol (glycerin, glycerine)
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

PI4KA also known as phosphatidylinositol 4-kinase alpha is an enzyme responsible for phosphorylating phosphatidylinositol to produce phosphatidylinositol 4-phosphate (PI4P). The protein has a mass of approximately 230 kDa. PI4KA is expressed broadly with significant levels in the brain heart and other tissues. It plays an important role as a regulator of cellular lipid metabolism and intracellular signaling.
Biological function summary

PI4KA functions in the context of membrane dynamics and acts as part of a multi-subunit complex. This protein is important in maintaining the proper lipid composition of the Golgi apparatus and other organelles which is essential for vesicle trafficking. By generating PI4P PI4KA serves as an important mediator in the distribution and dynamics of cellular membranes aiding in various cellular processes including endocytosis and autophagy. Its activity impacts several downstream signaling pathways and cellular activities.

Pathways

PI4KA is a significant contributor to the phosphoinositide signaling pathway which plays an essential role in regulating intracellular processes. It interacts with phosphatidylinositol-45-bisphosphate (PIP2) and phospholipase C supporting the synthesis of further derivatives that modulate calcium influx and other cellular responses. Additionally PI4KA is involved in the maintenance and function of the secretory pathway where it coordinates with proteins such as Osh1 and CERT influencing lipid exchange and protein trafficking.

PI4KA's dysfunction has been linked to neurological disorders and cancer. Alterations in its activity can contribute to conditions like neurodegenerative diseases due to disrupted membrane composition in neural tissues. Furthermore PI4KA's role in cancer is illuminated through its interaction with proteins like AKT where aberrant PI4P levels disrupt normal cell growth signals leading to oncogenesis. Understanding PI4KA's pathways and interactions is critical as they offer potential therapeutic targets for these diseases.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Acts on phosphatidylinositol (PtdIns) in the first committed step in the production of the second messenger inositol-1,4,5,-trisphosphate.
See full target information PI4KA
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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

International journal of biological sciences 18:4963-4983 PubMed35982909

2022

Human promotes growth and metastasis via sphingolipid metabolite ceramide and PI4KA/AKT signaling in pancreatic cancer cells.

Applications

Unspecified application

Species

Unspecified reactive species

Yanqun Zhang,Shenying Ji,Xiangyu Zhang,Mengyun Lu,Yihong Hu,Yucheng Han,Guanghou Shui,Sin Man Lam,Xianqiong Zou

BioMed research international 2020:9692503 PubMed32964048

2020

Phosphatidylinositol-4,5-Biphosphate (PI(4,5)P) Is Required for Rapid Endocytosis in Chromaffin Cells.

Applications

Unspecified application

Species

Unspecified reactive species

Fouad Azizi
View all publications
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions
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Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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