AB263970

Anti-PIAS1 antibody

Name: Anti-PIAS1 antibody (ab263970) | Abcam
Brand: Abcam Limited
SKU: AB263970
Price: 525 USD
Availability: InStock

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(1 Publication)

Rabbit Polyclonal PIAS1 antibody. Suitable for WB and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Synthetic Peptide within Human PIAS1.

View Alternative Names

DDXBP1, PIAS1, E3 SUMO-protein ligase PIAS1, DEAD/H box-binding protein 1, E3 SUMO-protein transferase PIAS1, Gu-binding protein, Protein inhibitor of activated STAT protein 1, RNA helicase II-binding protein, GBP

1 Images

Supplier Data

Western blot - Anti-PIAS1 antibody (AB263970)

Primary incubation overnight at 4°C.

Secondary incubation for 45 minutes at RT.

All lanes:

Western blot - Anti-PIAS1 antibody (ab263970) at 1 µg/mL

Lane 1:

HEK-293 (human epithelial cell line from embryonic kidney) whole cell lysate

Lane 2:

K562 (human chronic myelogenous leukemia cell line from bone marrow ) whole cell lysate

Secondary

All lanes:

Rabbit secondary HRP at 1/70000 dilution

Predicted band size: 71 kDa

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Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

applications

Immunogen

Synthetic Peptide within Human PIAS1. The exact immunogen used to generate this antibody is proprietary information.

O75925

Specificity

A BLAST analysis was used to suggest reactivity to mouse 92.9% homology for the immunogen sequence. Cross-reactivity from PIAS1 family and other sources has not been determined.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Purification notes

Anti-PIAS1 Antibody was affinity purified from monospecific antiserum by immunoaffinity chromatography.

Storage buffer

pH: 7.2 Preservative: 0.01% Sodium azide Constituents: 1.46% Sodium chloride, 0.164% Sodium phosphate

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

PIAS1 also known as Protein Inhibitor of Activated STAT 1 functions as a E3 SUMO-protein ligase which regulates protein function and localization through sumoylation. It has a molecular mass of approximately 70 kDa. PIAS1 is widely expressed in a variety of tissues indicating its involvement in diverse cellular functions. This protein binds to target proteins and modulates their activity impacting how cells respond to different stimuli.

Biological function summary

PIAS1 influences transcription regulation and gene expression. It acts as a transcriptional co-regulator by interacting with various transcription factors and modulating their activity. PIAS1 is part of multiprotein complexes that mediate these regulatory processes. Its activity is important for maintaining a balanced gene expression which if uncontrolled may result in abnormal cell behavior.

Pathways

PIAS1 plays an important role in signal transduction pathways such as the JAK/STAT pathway and the NF-kB signaling pathway. It interacts with proteins like STAT an important component in these pathways to regulate immune responses and cell growth. PIAS1 modulates the activity of these pathways in part by the covalent attachment of the small ubiquitin-like modifier (SUMO) proteins demonstrating its importance in cellular signaling networks.

PIAS1 relates closely to cancer progression and autoimmune diseases. Abnormal PIAS1 function or expression has links to certain cancers due to its role in controlling cell proliferation and differentiation. In autoimmune disorders PIAS1 affects immune regulation through its interaction with Signal Transducer and Activator of Transcription proteins showing altered activity in diseases like rheumatoid arthritis. Understanding PIAS1's contributions to these pathological states helps in developing targeted therapies.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Functions as an E3-type small ubiquitin-like modifier (SUMO) ligase, stabilizing the interaction between UBE2I and the substrate, and as a SUMO-tethering factor (PubMed : 11583632, PubMed : 11867732, PubMed : 14500712, PubMed : 21965678, PubMed : 36050397). Catalyzes sumoylation of various proteins, such as CEBPB, MRE11, MTA1, PTK2 and PML (PubMed : 11583632, PubMed : 11867732, PubMed : 14500712, PubMed : 21965678, PubMed : 36050397). Plays a crucial role as a transcriptional coregulation in various cellular pathways, including the STAT pathway, the p53 pathway and the steroid hormone signaling pathway (PubMed : 11583632, PubMed : 11867732). In vitro, binds A/T-rich DNA (PubMed : 15133049). The effects of this transcriptional coregulation, transactivation or silencing, may vary depending upon the biological context (PubMed : 11583632, PubMed : 11867732, PubMed : 14500712, PubMed : 21965678, PubMed : 36050397). Mediates sumoylation of MRE11, stabilizing MRE11 on chromatin during end resection (PubMed : 36050397). Sumoylates PML (at 'Lys-65' and 'Lys-160') and PML-RAR and promotes their ubiquitin-mediated degradation (By similarity). PIAS1-mediated sumoylation of PML promotes its interaction with CSNK2A1/CK2 which in turn promotes PML phosphorylation and degradation (By similarity). Enhances the sumoylation of MTA1 and may participate in its paralog-selective sumoylation (PubMed : 21965678). Plays a dynamic role in adipogenesis by promoting the SUMOylation and degradation of CEBPB (By similarity). Mediates the nuclear mobility and localization of MSX1 to the nuclear periphery, whereby MSX1 is brought into the proximity of target myoblast differentiation factor genes (By similarity). Also required for the binding of MSX1 to the core enhancer region in target gene promoter regions, independent of its sumoylation activity (By similarity). Capable of binding to the core enhancer region TAAT box in the MYOD1 gene promoter (By similarity).. (Microbial infection) Restricts Epstein-Barr virus (EBV) lytic replication by acting as an inhibitor for transcription factors involved in lytic gene expression (PubMed : 29262325). The virus can use apoptotic caspases to antagonize PIAS1-mediated restriction and express its lytic genes (PubMed : 29262325).

See full target information PIAS1

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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Human genetics 143:683-694 PubMed38592547

2024

Loss-of-function variants affecting the STAGA complex component SUPT7L cause a developmental disorder with generalized lipodystrophy.

Applications

Unspecified application

Species

Unspecified reactive species

Johannes Kopp,Leonard A Koch,Hristiana Lyubenova,Oliver Küchler,Manuel Holtgrewe,Andranik Ivanov,Christele Dubourg,Erika Launay,Sebastian Brachs,Stefan Mundlos,Nadja Ehmke,Dominik Seelow,Mélanie Fradin,Uwe Kornak,Björn Fischer-Zirnsak

View all publications

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Product promise

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Anti-PIAS1 antibody

Western blot - Anti-PIAS1 antibody (AB263970)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Specificity

Reactivity data

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (1)

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