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AB77231

Anti-PIAS1 + PIAS2 antibody [EPR2581Y]

4

(4 Reviews)

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(21 Publications)

Rabbit Recombinant Monoclonal PIAS1 antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Human, Synthetic peptide - Human, Mouse, Rat samples. Cited in 21 publications.

View Alternative Names

DDXBP1, PIAS1, E3 SUMO-protein ligase PIAS1, DEAD/H box-binding protein 1, E3 SUMO-protein transferase PIAS1, Gu-binding protein, Protein inhibitor of activated STAT protein 1, RNA helicase II-binding protein, GBP

4 Images
Immunocytochemistry/ Immunofluorescence - Anti-PIAS1 + PIAS2 antibody [EPR2581Y] (AB77231)
  • ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-PIAS1 + PIAS2 antibody [EPR2581Y] (AB77231)

Immunocytochemistry/Immunofluorescence analysis of HeLa (human cervix adenocarcinoma) cells labelling PIAS1 + PIAS2 with purified ab77231 at 1/500. Cells were fixed with 4% Paraformaldehyde and permeabilized with 0.1% Triton X-100. ab150077 Alexa Fluor® 488-conjugated goat anti-rabbit IgG (1/1000) was used as the secondary antibody. Nuclei were counterstained with DAPI (blue).

Secondary Only Control : PBS was used instead of the primary antibody as the negative control.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PIAS1 + PIAS2 antibody [EPR2581Y] (AB77231)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PIAS1 + PIAS2 antibody [EPR2581Y] (AB77231)

ab77231 at a 1/100 dilution staining PIAS1 + PIAS2 in paraffin embedded human tonsil tissue by Immunohistochemistry.

Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol.

Western blot - Anti-PIAS1 + PIAS2 antibody [EPR2581Y] (AB77231)
  • WB

Unknown

Western blot - Anti-PIAS1 + PIAS2 antibody [EPR2581Y] (AB77231)

All lanes:

Western blot - Anti-PIAS1 + PIAS2 antibody [EPR2581Y] (ab77231) at 1/50000 dilution

Lane 1:

Daudi cell lysate at 10 µg

Lane 2:

293 cell lysate at 10 µg

Lane 3:

Jurkat cell lysate at 10 µg

Lane 4:

SW40 cell lysate at 10 µg

Lane 5:

HepG2 cell lysate at 10 µg

Secondary

All lanes:

HRP labelled goat anti-rabbit at 1/2000 dilution

Predicted band size: 72 kDa

Observed band size: 78 kDa

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Western blot - Anti-PIAS1 + PIAS2 antibody [EPR2581Y] (AB77231)
  • WB

Supplier Data

Western blot - Anti-PIAS1 + PIAS2 antibody [EPR2581Y] (AB77231)

Blocking and Diluting buffer and concentration : 5% NFDM /TBST

Exposure time : Lane1 : 10 seconds, Lane2 : 5 seconds, Lane3 : 3 minutes.

Human PIAS1 full-length recombinant protein (Cat# : ab152888)

All lanes:

Western blot - Anti-PIAS1 + PIAS2 antibody [EPR2581Y] (ab77231) at 1/50000 dilution

Lane 1:

Human PIAS1 full-length recombinant protein with GST-Tag at 0.015 µg

Lane 2:

Human PIAS2-beta recombinant protein fragment with GST-His-Tag at 0.015 µg

Lane 3:

Human PIAS3 recombinant protein fragment with SUMO-His-Tag at 0.015 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Predicted band size: 72 kDa

Observed band size: 42 kDa,98 kDa

false

  • Carrier free

    Anti-PIAS1+PIAS2 antibody [EPR2581Y] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR2581Y

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

WB, ICC/IF, IHC-P

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/250", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100 - 1/500", "ICCIF-species-notes": "<p></p>" }, "Mouse": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Synthetic peptide - Human": { "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }
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Product details

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

PIAS1 and PIAS2 which stand for Protein Inhibitor of Activated STAT1 and STAT2 respectively are members of the PIAS protein family. These proteins are known to function as E3 SUMO-protein ligases and play a significant role in the post-translational modification process called SUMOylation. PIAS1 has a mass of about 71 kDa and PIAS2 has a mass of approximately 73 kDa. PIAS1 and PIAS2 are expressed in various tissues with high expression observed in the brain and hematopoietic cells.
Biological function summary

PIAS1 and PIAS2 play essential roles in modulating key signaling pathways through SUMOylation. They often work as part of larger protein complexes modulating the activity of transcription factors such as STAT p53 and NF-kB. By affecting these transcription factors PIAS proteins can regulate gene expression thereby influencing cell proliferation differentiation and response to stress. This involvement in gene regulation underlines their importance in maintaining cellular homeostasis.

Pathways

PIAS1 and PIAS2 contribute to critical processes like the JAK-STAT signaling pathway and the NF-kB signaling pathway. These pathways are influential in controlling immune responses. Through phosphorylation and interaction with other transcription factors like STAT1 and NF-kB PIAS proteins alter the transcriptional activity of genes involved in inflammation and cellular stress responses. The modulation of these pathways links PIAS1 and PIAS2 with both immune and stress responses playing a role in both normal and pathological conditions.

PIAS1 and PIAS2 are associated with chronic inflammation and cancer. Their regulatory role in the JAK-STAT and NF-kB pathways connects them to inflammatory diseases where dysregulation of these pathways is common. Additionally PIAS1 and PIAS2 interact with proteins like p53 linking them to tumor suppression and anomalies in their expression or function can contribute to the development of cancers. Understanding these associations is important for developing therapies targeting these signaling proteins.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Functions as an E3-type small ubiquitin-like modifier (SUMO) ligase, stabilizing the interaction between UBE2I and the substrate, and as a SUMO-tethering factor (PubMed : 11583632, PubMed : 11867732, PubMed : 14500712, PubMed : 21965678, PubMed : 36050397). Catalyzes sumoylation of various proteins, such as CEBPB, MRE11, MTA1, PTK2 and PML (PubMed : 11583632, PubMed : 11867732, PubMed : 14500712, PubMed : 21965678, PubMed : 36050397). Plays a crucial role as a transcriptional coregulation in various cellular pathways, including the STAT pathway, the p53 pathway and the steroid hormone signaling pathway (PubMed : 11583632, PubMed : 11867732). In vitro, binds A/T-rich DNA (PubMed : 15133049). The effects of this transcriptional coregulation, transactivation or silencing, may vary depending upon the biological context (PubMed : 11583632, PubMed : 11867732, PubMed : 14500712, PubMed : 21965678, PubMed : 36050397). Mediates sumoylation of MRE11, stabilizing MRE11 on chromatin during end resection (PubMed : 36050397). Sumoylates PML (at 'Lys-65' and 'Lys-160') and PML-RAR and promotes their ubiquitin-mediated degradation (By similarity). PIAS1-mediated sumoylation of PML promotes its interaction with CSNK2A1/CK2 which in turn promotes PML phosphorylation and degradation (By similarity). Enhances the sumoylation of MTA1 and may participate in its paralog-selective sumoylation (PubMed : 21965678). Plays a dynamic role in adipogenesis by promoting the SUMOylation and degradation of CEBPB (By similarity). Mediates the nuclear mobility and localization of MSX1 to the nuclear periphery, whereby MSX1 is brought into the proximity of target myoblast differentiation factor genes (By similarity). Also required for the binding of MSX1 to the core enhancer region in target gene promoter regions, independent of its sumoylation activity (By similarity). Capable of binding to the core enhancer region TAAT box in the MYOD1 gene promoter (By similarity).. (Microbial infection) Restricts Epstein-Barr virus (EBV) lytic replication by acting as an inhibitor for transcription factors involved in lytic gene expression (PubMed : 29262325). The virus can use apoptotic caspases to antagonize PIAS1-mediated restriction and express its lytic genes (PubMed : 29262325).
See full target information PIAS1

Additional targets

PIAS2
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Publications (21)

Recent publications for all applications. Explore the full list and refine your search

Cell death & disease 15:674 PubMed39277582

2024

Impaired SUMOylation of FoxA1 promotes nonalcoholic fatty liver disease through down-regulation of Sirt6.

Applications

Unspecified application

Species

Unspecified reactive species

Dongmei Zou,Jinwen Liao,Min Xiao,Liang Liu,Dongling Dai,Mingguo Xu

Nature structural & molecular biology 31:1355-1367 PubMed38649616

2024

Concerted SUMO-targeted ubiquitin ligase activities of TOPORS and RNF4 are essential for stress management and cell proliferation.

Applications

Unspecified application

Species

Unspecified reactive species

Julio C Y Liu,Leena Ackermann,Saskia Hoffmann,Zita Gál,Ivo A Hendriks,Charu Jain,Louise Morlot,Michael H Tatham,Gian-Luca McLelland,Ronald T Hay,Michael Lund Nielsen,Thijn Brummelkamp,Peter Haahr,Niels Mailand

mBio 15:e0316823 PubMed38236021

2024

SUMOylation of the m6A reader YTHDF2 by PIAS1 promotes viral RNA decay to restrict EBV replication.

Applications

Unspecified application

Species

Unspecified reactive species

Febri Gunawan Sugiokto,Farjana Saiada,Kun Zhang,Renfeng Li

Cell reports 37:110127 PubMed34936874

2021

PAK5 promotes RNA helicase DDX5 sumoylation and miRNA-10b processing in a kinase-dependent manner in breast cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Yang Li,Yao Xing,Xu Wang,Bingtao Hu,Xin Zhao,Hongyan Zhang,Fuyi Han,Nanxi Geng,Fei Wang,Yanshu Li,Jiabin Li,Feng Jin,Feng Li

Nucleic acids research : PubMed33524141

2021

SUMOylation regulates the protein network and chromatin accessibility at glucocorticoid receptor-binding sites.

Applications

Unspecified application

Species

Unspecified reactive species

Ville Paakinaho,Joanna K Lempiäinen,Gianluca Sigismondo,Einari A Niskanen,Marjo Malinen,Tiina Jääskeläinen,Markku Varjosalo,Jeroen Krijgsveld,Jorma J Palvimo

The Journal of biological chemistry 295:8350-8362 PubMed32350109

2020

Multisite SUMOylation restrains DNA polymerase η interactions with DNA damage sites.

Applications

Unspecified application

Species

Unspecified reactive species

Claire Guérillon,Stine Smedegaard,Ivo A Hendriks,Michael L Nielsen,Niels Mailand

Virology 540:75-87 PubMed31743858

2019

Protein inhibitor of activated STAT1 (PIAS1) inhibits IRF8 activation of Epstein-Barr virus lytic gene expression.

Applications

Unspecified application

Species

Unspecified reactive species

Kun Zhang,Dong-Wen Lv,Renfeng Li

Oncoimmunology 8:e1663107 PubMed31741759

2019

T cell recognition of novel shared breast cancer antigens is frequently observed in peripheral blood of breast cancer patients.

Applications

Unspecified application

Species

Unspecified reactive species

Nadia Viborg,Sofie Ramskov,Rikke Sick Andersen,Theo Sturm,Tim Fugmann,Amalie Kai Bentzen,Vibeke Mindahl Rafa,Per Thor Straten,Inge Marie Svane,Özcan Met,Sine Reker Hadrup

PloS one 14:e0212857 PubMed30794696

2019

Developmental profiles of SUMOylation pathway proteins in rat cerebrum and cerebellum.

Applications

Unspecified application

Species

Unspecified reactive species

Fernando Josa-Prado,Jia Luo,Philip Rubin,Jeremy M Henley,Kevin A Wilkinson

BMC biology 15:112 PubMed29183317

2017

Smad4 SUMOylation is essential for memory formation through upregulation of the skeletal myopathy gene TPM2.

Applications

Unspecified application

Species

Unspecified reactive species

Wei L Hsu,Yun L Ma,Yen C Liu,Eminy H Y Lee
View all publications
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