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Rabbit Recombinant Monoclonal PIAS1 antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Human, Synthetic peptide - Human, Mouse, Rat samples. Cited in 18 publications.


Images

Western blot - Anti-PIAS1 + PIAS2 antibody [EPR2581Y] (AB77231), expandable thumbnail
  • Western blot - Anti-PIAS1 + PIAS2 antibody [EPR2581Y] (AB77231), expandable thumbnail
  • Immunocytochemistry/ Immunofluorescence - Anti-PIAS1 + PIAS2 antibody [EPR2581Y] (AB77231), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PIAS1 + PIAS2 antibody [EPR2581Y] (AB77231), expandable thumbnail

Publications

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 9% PBS, 0.05% BSA

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IHC-PWBICC/IF
Human
Tested
Tested
Tested
Mouse
Predicted
Expected
Predicted
Rat
Predicted
Expected
Predicted
Synthetic peptide - Human
Not recommended
Tested
Not recommended

Tested
Tested

Species
Human
Dilution info
1/100 - 1/250
Notes

Perform heat-mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol.

Predicted
Predicted

Species
Mouse, Rat
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Synthetic peptide - Human
Dilution info
-
Notes

-

Tested
Tested

Species
Synthetic peptide - Human
Dilution info
1/1000 - 1/10000
Notes

-

Species
Human
Dilution info
1/1000 - 1/10000
Notes

-

Expected
Expected

Species
Mouse, Rat
Dilution info
Use at an assay dependent concentration.
Notes

-

Tested
Tested

Species
Human
Dilution info
1/100 - 1/500
Notes

-

Predicted
Predicted

Species
Mouse, Rat
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Synthetic peptide - Human
Dilution info
-
Notes

-

Target data

Function

Functions as an E3-type small ubiquitin-like modifier (SUMO) ligase, stabilizing the interaction between UBE2I and the substrate, and as a SUMO-tethering factor (PubMed:11583632, PubMed:11867732, PubMed:14500712, PubMed:21965678, PubMed:36050397). Catalyzes sumoylation of various proteins, such as CEBPB, MRE11, MTA1, PTK2 and PML (PubMed:11583632, PubMed:11867732, PubMed:14500712, PubMed:21965678, PubMed:36050397). Plays a crucial role as a transcriptional coregulation in various cellular pathways, including the STAT pathway, the p53 pathway and the steroid hormone signaling pathway (PubMed:11583632, PubMed:11867732). In vitro, binds A/T-rich DNA (PubMed:15133049). The effects of this transcriptional coregulation, transactivation or silencing, may vary depending upon the biological context (PubMed:11583632, PubMed:11867732, PubMed:14500712, PubMed:21965678, PubMed:36050397). Mediates sumoylation of MRE11, stabilizing MRE11 on chromatin during end resection (PubMed:36050397). Sumoylates PML (at 'Lys-65' and 'Lys-160') and PML-RAR and promotes their ubiquitin-mediated degradation (By similarity). PIAS1-mediated sumoylation of PML promotes its interaction with CSNK2A1/CK2 which in turn promotes PML phosphorylation and degradation (By similarity). Enhances the sumoylation of MTA1 and may participate in its paralog-selective sumoylation (PubMed:21965678). Plays a dynamic role in adipogenesis by promoting the SUMOylation and degradation of CEBPB (By similarity). Mediates the nuclear mobility and localization of MSX1 to the nuclear periphery, whereby MSX1 is brought into the proximity of target myoblast differentiation factor genes (By similarity). Also required for the binding of MSX1 to the core enhancer region in target gene promoter regions, independent of its sumoylation activity (By similarity). Capable of binding to the core enhancer region TAAT box in the MYOD1 gene promoter (By similarity). (Microbial infection) Restricts Epstein-Barr virus (EBV) lytic replication by acting as an inhibitor for transcription factors involved in lytic gene expression (PubMed:29262325). The virus can use apoptotic caspases to antagonize PIAS1-mediated restriction and express its lytic genes (PubMed:29262325).

Additional Targets

PIAS2

Alternative names

DDXBP1, PIAS1, E3 SUMO-protein ligase PIAS1, DEAD/H box-binding protein 1, E3 SUMO-protein transferase PIAS1, Gu-binding protein, Protein inhibitor of activated STAT protein 1, RNA helicase II-binding protein, GBP

Recommended products

Rabbit Recombinant Monoclonal PIAS1 antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Human, Synthetic peptide - Human, Mouse, Rat samples. Cited in 18 publications.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number
EPR2581Y
Purification technique
Affinity purification Protein A
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle

Notes

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

PIAS1 and PIAS2 which stand for Protein Inhibitor of Activated STAT1 and STAT2 respectively are members of the PIAS protein family. These proteins are known to function as E3 SUMO-protein ligases and play a significant role in the post-translational modification process called SUMOylation. PIAS1 has a mass of about 71 kDa and PIAS2 has a mass of approximately 73 kDa. PIAS1 and PIAS2 are expressed in various tissues with high expression observed in the brain and hematopoietic cells.

Biological function summary

PIAS1 and PIAS2 play essential roles in modulating key signaling pathways through SUMOylation. They often work as part of larger protein complexes modulating the activity of transcription factors such as STAT p53 and NF-kB. By affecting these transcription factors PIAS proteins can regulate gene expression thereby influencing cell proliferation differentiation and response to stress. This involvement in gene regulation underlines their importance in maintaining cellular homeostasis.

Pathways

PIAS1 and PIAS2 contribute to critical processes like the JAK-STAT signaling pathway and the NF-kB signaling pathway. These pathways are influential in controlling immune responses. Through phosphorylation and interaction with other transcription factors like STAT1 and NF-kB PIAS proteins alter the transcriptional activity of genes involved in inflammation and cellular stress responses. The modulation of these pathways links PIAS1 and PIAS2 with both immune and stress responses playing a role in both normal and pathological conditions.

Associated diseases and disorders

PIAS1 and PIAS2 are associated with chronic inflammation and cancer. Their regulatory role in the JAK-STAT and NF-kB pathways connects them to inflammatory diseases where dysregulation of these pathways is common. Additionally PIAS1 and PIAS2 interact with proteins like p53 linking them to tumor suppression and anomalies in their expression or function can contribute to the development of cancers. Understanding these associations is important for developing therapies targeting these signaling proteins.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

4 product images

  • Western blot - Anti-PIAS1 + PIAS2 antibody [EPR2581Y] (ab77231), expandable thumbnail

    Western blot - Anti-PIAS1 + PIAS2 antibody [EPR2581Y] (ab77231)

    Blocking and Diluting buffer and concentration: 5% NFDM /TBST

    Exposure time: Lane1:10 seconds, Lane2: 5 seconds, Lane3: 3 minutes.

    Human PIAS1 full-length recombinant protein (Cat#: ab152888)

    All lanes: Western blot - Anti-PIAS1 + PIAS2 antibody [EPR2581Y] (ab77231) at 1/50000 dilution

    Lane 1: Human PIAS1 full-length recombinant protein with GST-Tag at 0.015 µg

    Lane 2: Human PIAS2-beta recombinant protein fragment with GST-His-Tag at 0.015 µg

    Lane 3: Human PIAS3 recombinant protein fragment with SUMO-His-Tag at 0.015 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution

    Predicted band size: 72 kDa

    Observed band size: 42 kDa, 98 kDa

  • Western blot - Anti-PIAS1 + PIAS2 antibody [EPR2581Y] (ab77231), expandable thumbnail

    Western blot - Anti-PIAS1 + PIAS2 antibody [EPR2581Y] (ab77231)

    All lanes: Western blot - Anti-PIAS1 + PIAS2 antibody [EPR2581Y] (ab77231) at 1/50000 dilution

    Lane 1: Daudi cell lysate at 10 µg

    Lane 2: 293 cell lysate at 10 µg

    Lane 3: Jurkat cell lysate at 10 µg

    Lane 4: SW40 cell lysate at 10 µg

    Lane 5: HepG2 cell lysate at 10 µg

    Secondary

    All lanes: HRP labelled goat anti-rabbit at 1/2000 dilution

    Predicted band size: 72 kDa

    Observed band size: 78 kDa

  • Immunocytochemistry/ Immunofluorescence - Anti-PIAS1 + PIAS2 antibody [EPR2581Y] (ab77231), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-PIAS1 + PIAS2 antibody [EPR2581Y] (ab77231)

    Immunocytochemistry/Immunofluorescence analysis of HeLa (human cervix adenocarcinoma) cells labelling PIAS1 + PIAS2 with purified ab77231 at 1/500. Cells were fixed with 4% Paraformaldehyde and permeabilized with 0.1% Triton X-100. Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077, Alexa Fluor® 488-conjugated goat anti-rabbit IgG (1/1000) was used as the secondary antibody. Nuclei were counterstained with DAPI (blue).

    Secondary Only Control: PBS was used instead of the primary antibody as the negative control.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PIAS1 + PIAS2 antibody [EPR2581Y] (ab77231), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PIAS1 + PIAS2 antibody [EPR2581Y] (ab77231)

    ab77231, at a 1/100 dilution, staining PIAS1 + PIAS2 in paraffin embedded human tonsil tissue by Immunohistochemistry.

    Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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