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AB247490

Anti-PIAS1+PIAS2 antibody [EPR2581Y] - BSA and Azide free

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(1 Publication)

Rabbit Recombinant Monoclonal PIAS1 antibody. Carrier free. Suitable for IHC-P, ICC/IF, WB and reacts with Human, Recombinant full length protein - Human samples. Cited in 1 publication.

View Alternative Names

DDXBP1, PIAS1, E3 SUMO-protein ligase PIAS1, DEAD/H box-binding protein 1, E3 SUMO-protein transferase PIAS1, Gu-binding protein, Protein inhibitor of activated STAT protein 1, RNA helicase II-binding protein, GBP, PIASX, PIAS2, E3 SUMO-protein ligase PIAS2, Androgen receptor-interacting protein 3, DAB2-interacting protein, E3 SUMO-protein transferase PIAS2, Msx-interacting zinc finger protein, PIAS-NY protein, Protein inhibitor of activated STAT x, Protein inhibitor of activated STAT2, ARIP3, DIP, Miz1

4 Images
Immunocytochemistry/ Immunofluorescence - Anti-PIAS1+PIAS2 antibody [EPR2581Y] - BSA and Azide free (AB247490)
  • ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-PIAS1+PIAS2 antibody [EPR2581Y] - BSA and Azide free (AB247490)

This data was developed using ab77231, the same antibody clone in a different buffer formulation.

Immunocytochemistry/Immunofluorescence analysis of HeLa (human cervix adenocarcinoma) cells labelling PIAS1 + PIAS2 with purified ab77231 at 1/500. Cells were fixed with 4% Paraformaldehyde and permeabilized with 0.1% Triton X-100. ab150077, Alexa Fluor® 488-conjugated goat anti-rabbit IgG (1/1000) was used as the secondary antibody. Nuclei were counterstained with DAPI (blue).

Secondary Only Control : PBS was used instead of the primary antibody as the negative control.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PIAS1+PIAS2 antibody [EPR2581Y] - BSA and Azide free (AB247490)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PIAS1+PIAS2 antibody [EPR2581Y] - BSA and Azide free (AB247490)

This data was developed using ab77231, the same antibody clone in a different buffer formulation.

ab77231, at a 1/100 dilution, staining PIAS1 + PIAS2 in paraffin embedded human tonsil tissue by Immunohistochemistry.

Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol.

Western blot - Anti-PIAS1+PIAS2 antibody [EPR2581Y] - BSA and Azide free (AB247490)
  • WB

Unknown

Western blot - Anti-PIAS1+PIAS2 antibody [EPR2581Y] - BSA and Azide free (AB247490)

This data was developed using ab77231, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-PIAS1 + PIAS2 antibody [EPR2581Y] (<a href='/en-us/products/primary-antibodies/pias1-pias2-antibody-epr2581y-ab77231'>ab77231</a>) at 1/50000 dilution

Lane 1:

Daudi cell lysate at 10 µg

Lane 2:

293 cell lysate at 10 µg

Lane 3:

Jurkat cell lysate at 10 µg

Lane 4:

SW40 cell lysate at 10 µg

Lane 5:

HepG2 cell lysate at 10 µg

Secondary

All lanes:

HRP labelled goat anti-rabbit at 1/2000 dilution

Predicted band size: 72 kDa

Observed band size: 78 kDa

false

Western blot - Anti-PIAS1+PIAS2 antibody [EPR2581Y] - BSA and Azide free (AB247490)
  • WB

Supplier Data

Western blot - Anti-PIAS1+PIAS2 antibody [EPR2581Y] - BSA and Azide free (AB247490)

This data was developed using ab77231, the same antibody clone in a different buffer formulation.

Blocking and dilution buffer : 5% NFDM/TBST.

Exposure times : Lane 1 : 10 seconds, Lane 2 : 5 seconds, Lane 3 : 3 minutes.

Human PIAS1 full-length recombinant protein (ab152888)

All lanes:

Western blot - Anti-PIAS1 + PIAS2 antibody [EPR2581Y] (<a href='/en-us/products/primary-antibodies/pias1-pias2-antibody-epr2581y-ab77231'>ab77231</a>) at 1/50000 dilution

Lane 1:

Human PIAS1 full-length recombinant protein with GST-Tag at 0.015 µg

Lane 2:

Human PIAS2-beta recombinant protein fragment with GST-His-Tag at 0.015 µg

Lane 3:

Human PIAS3 recombinant protein fragment with SUMO-His-Tag at 0.015 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Predicted band size: 72 kDa

Observed band size: 42 kDa,98 kDa

false

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR2581Y

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

ICC/IF, WB, IHC-P

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SAE2 / UBA2 antibody [EPR14880] (AB185955)

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Secondary Antibodies

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Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488)

Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (AB150077)

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Primary Antibodies

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol.", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>" }, "Mouse": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rat": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Recombinant full length protein - Human": { "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>" } } }
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Product details

ab247490 is the carrier-free version of ab77231.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

PIAS1 and PIAS2 are known as Protein Inhibitors of Activated STAT. PIAS1 has a mass of approximately 72 kDa while PIAS2 is around 66 kDa. These proteins are present in the nucleus and they regulate various transcription factors. They achieve this by attaching SUMO (Small Ubiquitin-like Modifier) proteins to specific substrates a process called SUMOylation. Both PIAS1 and PIAS2 express in various tissues but expression levels can vary with cell type and physiological conditions.
Biological function summary

PIAS1 and PIAS2 proteins play roles in the regulation of gene transcription. They can form complexes with several transcription factors and alter their activity by SUMOylating them. This modification is important because it changes how these transcription factors interact with DNA and other proteins. PIAS proteins sometimes act as co-regulators by either enhancing or repressing transcription factor function. This dual ability influences cellular processes like cytokine signaling and the immune response.

Pathways

PIAS1 and PIAS2 participate in the JAK-STAT signaling pathway which is critical for transmitting signals from cytokines to the cell nucleus. It involves the activation of STAT family proteins such as STAT1 and STAT3. PIAS proteins add a layer of regulation influencing cell growth and differentiation. Another pathway connected with these proteins is the p53 pathway where PIAS1 can interact with p53 to regulate apoptosis and cell cycle arrest. These interactions indicate the versatile roles of PIAS proteins in cellular homeostasis.

PIAS1 and PIAS2 have links to cancer and autoimmune diseases. For example abnormal sumoylation mediated by these proteins can contribute to oncogenesis by disrupting normal cell cycle control involving key players like p53. In autoimmune conditions PIAS proteins may alter immune response through changes in inflammation-related genes. Knowing the interaction of PIAS proteins with transcription factors such as STAT1 helps to understand the complex gene regulation changes in these diseases.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Functions as an E3-type small ubiquitin-like modifier (SUMO) ligase, stabilizing the interaction between UBE2I and the substrate, and as a SUMO-tethering factor (PubMed : 11583632, PubMed : 11867732, PubMed : 14500712, PubMed : 15280358, PubMed : 21965678, PubMed : 36050397). Catalyzes sumoylation of various proteins, such as CEBPB, MRE11, MTA1, PTK2, PML and ZNF76 (PubMed : 11583632, PubMed : 11867732, PubMed : 14500712, PubMed : 15280358, PubMed : 21965678, PubMed : 36050397). Plays a crucial role as a transcriptional coregulation in various cellular pathways, including the STAT pathway, the p53 pathway and the steroid hormone signaling pathway (PubMed : 11583632, PubMed : 11867732). In vitro, binds A/T-rich DNA (PubMed : 15133049). The effects of this transcriptional coregulation, transactivation or silencing, may vary depending upon the biological context (PubMed : 11583632, PubMed : 11867732, PubMed : 14500712, PubMed : 21965678, PubMed : 36050397). Mediates sumoylation of MRE11, stabilizing MRE11 on chromatin during end resection (PubMed : 36050397). Sumoylates PML (at 'Lys-65' and 'Lys-160') and PML-RAR and promotes their ubiquitin-mediated degradation (By similarity). PIAS1-mediated sumoylation of PML promotes its interaction with CSNK2A1/CK2 which in turn promotes PML phosphorylation and degradation (By similarity). Enhances the sumoylation of MTA1 and may participate in its paralog-selective sumoylation (PubMed : 21965678). Plays a dynamic role in adipogenesis by promoting the SUMOylation and degradation of CEBPB (By similarity). Mediates the nuclear mobility and localization of MSX1 to the nuclear periphery, whereby MSX1 is brought into the proximity of target myoblast differentiation factor genes (By similarity). Also required for the binding of MSX1 to the core enhancer region in target gene promoter regions, independent of its sumoylation activity (By similarity). Capable of binding to the core enhancer region TAAT box in the MYOD1 gene promoter (By similarity).. (Microbial infection) Restricts Epstein-Barr virus (EBV) lytic replication by acting as an inhibitor for transcription factors involved in lytic gene expression (PubMed : 29262325). The virus can use apoptotic caspases to antagonize PIAS1-mediated restriction and express its lytic genes (PubMed : 29262325).
See full target information PIAS1

Additional targets

PIAS2
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Nature genetics 57:1142-1154 PubMed40229600

2025

Longitudinal single-cell multiomic atlas of high-risk neuroblastoma reveals chemotherapy-induced tumor microenvironment rewiring.

Applications

Unspecified application

Species

Unspecified reactive species

Wenbao Yu,Rumeysa Biyik-Sit,Yasin Uzun,Chia-Hui Chen,Anusha Thadi,Jonathan H Sussman,Minxing Pang,Chi-Yun Wu,Liron D Grossmann,Peng Gao,David W Wu,Aliza Yousey,Mei Zhang,Christina S Turn,Zhan Zhang,Shovik Bandyopadhyay,Jeffrey Huang,Tasleema Patel,Changya Chen,Daniel Martinez,Lea F Surrey,Michael D Hogarty,Kathrin Bernt,Nancy R Zhang,John M Maris,Kai Tan
View all publications
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

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