AB126601

Anti-PIAS2 antibody [EPR2582(2)]

RabMAb
Recombinant
What is this?

Be the first to review this product! Submit a review

(4 Publications)

Rabbit Recombinant Monoclonal PIAS2 antibody. Suitable for WB and reacts with Human samples. Cited in 4 publications.

View Alternative Names

PIASX, PIAS2, E3 SUMO-protein ligase PIAS2, Androgen receptor-interacting protein 3, DAB2-interacting protein, E3 SUMO-protein transferase PIAS2, Msx-interacting zinc finger protein, PIAS-NY protein, Protein inhibitor of activated STAT x, Protein inhibitor of activated STAT2, ARIP3, DIP, Miz1

1 Images

Unknown

Western blot - Anti-PIAS2 antibody [EPR2582(2)] (AB126601)

All lanes:

Western blot - Anti-PIAS2 antibody [EPR2582(2)] (ab126601) at 1/1000 dilution

Lane 1:

HepG2 cell lysate at 10 µg

Lane 2:

Human fetal kidney tissue lysate at 10 µg

Secondary

All lanes:

Goat anti-Rabbit HRP at 1/2000 dilution

Predicted band size: 68 kDa

false

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR2582(2)

Isotype

IgG

Carrier free

Reacts with

Human

Applications

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

style

left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Mouse": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Rat": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }

style

left-column

Product details

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

style

left-column

Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Storage information

Stable for 12 months at -20°C

style

left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The protein PIAS2 also known as Protein Inhibitor of Activated STAT2 plays an active role in regulating gene expression by modifying the activity of specific transcription factors. It is a member of the PIAS family and its molecular mass is approximately 71 kDa. Scientists have observed PIAS2 expression in various tissues including the brain heart and skeletal muscle suggesting its widespread importance across different cell types. This protein can bind to specific target proteins exerting its influence primarily through SUMOylation an essential post-translational modification process.

Biological function summary

PIAS2 regulates several cellular processes by modulating transcription factor activity. It acts as a co-regulator in various cellular contexts forming part of larger protein complexes. Through these interactions it manages immune responses cell cycle progression and apoptosis. PIAS2 is particularly active in controlling the balance of STAT signaling which affects numerous biological functions. By enhancing or repressing particular transcription factors PIAS2 can shape cellular outcomes important for normal development and function.

Pathways

PIAS2 plays an important role in controlling important signaling cascades including the JAK/STAT and TGF-beta pathways. Within these pathways it influences diverse biological processes from immune regulation to tissue repair. PIAS2 interacts with other proteins like STAT2 and SMADs that participate in these pathways integrating signals it receives and transmitting them to execute physiological responses. These interactions highlight PIAS2's multifaceted roles in mediating signal transduction and maintaining cellular homeostasis.

PIAS2 displays connections to cancer and autoimmune diseases. Altered PIAS2 function or expression can lead to aberrations in STAT signaling which may contribute to oncogenesis or impaired immune regulatory mechanisms. It has interactions with proteins such as p53 and STAT factors which link PIAS2 to pathways frequently disrupted in these conditions. Understanding PIAS2's involvement in these diseases highlights the potential for therapeutic targeting to mitigate disease progression or augment treatment strategies.

style

left-column

style

left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

style

left-column

Target data

Functions as an E3-type small ubiquitin-like modifier (SUMO) ligase, stabilizing the interaction between UBE2I and the substrate, and as a SUMO-tethering factor. Plays a crucial role as a transcriptional coregulator in various cellular pathways, including the STAT pathway, the p53 pathway and the steroid hormone signaling pathway. The effects of this transcriptional coregulation, transactivation or silencing may vary depending upon the biological context and the PIAS2 isoform studied. However, it seems to be mostly involved in gene silencing. Binds to sumoylated ELK1 and enhances its transcriptional activity by preventing recruitment of HDAC2 by ELK1, thus reversing SUMO-mediated repression of ELK1 transactivation activity. Isoform PIAS2-beta, but not isoform PIAS2-alpha, promotes MDM2 sumoylation. Isoform PIAS2-alpha promotes PARK7 sumoylation. Isoform PIAS2-beta promotes NCOA2 sumoylation more efficiently than isoform PIAS2-alpha. Isoform PIAS2-alpha sumoylates PML at'Lys-65' and 'Lys-160'.

See full target information PIAS2

style

left-column

Publications (4)

Recent publications for all applications. Explore the full list and refine your search

Aging cell 24:e14349 PubMed39380368

2024

CaMKIIα-TARPγ8 signaling mediates hippocampal synaptic impairment in aging.

Applications

Unspecified application

Species

Unspecified reactive species

Zhao JianHua,MingCan Li,Qilin Hu,Peter Donoghue,Sanwei Jiang,Junmei Li,Songji Li,Xinyi Ren,Ziyuan Zhang,Jingzhi Du,Yi Yu,Paul Chazot,Chengbiao Lu

Journal of proteome research 22:812-825 PubMed36723483

2023

SUMO Proteomics Analyses Identify Protein Inhibitor of Activated STAT-Mediated Regulatory Networks Involved in Cell Cycle and Cell Proliferation.

Applications

Unspecified application

Species

Unspecified reactive species

Chongyang Li,Alison Boutet,Cristina Mirela Pascariu,Trent Nelson,Mathieu Courcelles,Zhaoguan Wu,Simon Comtois-Marotte,Gregory Emery,Pierre Thibault

Nature communications 11:834 PubMed32047143

2020

Quantitative SUMO proteomics identifies PIAS1 substrates involved in cell migration and motility.

Applications

Unspecified application

Species

Unspecified reactive species

Chongyang Li,Francis P McManus,Cédric Plutoni,Cristina Mirela Pascariu,Trent Nelson,Lara Elis Alberici Delsin,Gregory Emery,Pierre Thibault

The Journal of biological chemistry 294:15218-15234 PubMed31285264

2019

Inhibiting ubiquitination causes an accumulation of SUMOylated newly synthesized nuclear proteins at PML bodies.

Applications

Unspecified application

Species

Unspecified reactive species

Zhe Sha,Tamara Blyszcz,Román González-Prieto,Alfred C O Vertegaal,Alfred L Goldberg

View all publications

style

left-column

style

left-column

style

right-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-PIAS2 antibody [EPR2582(2)]

Western blot - Anti-PIAS2 antibody [EPR2582(2)] (AB126601)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (4)

Alternative Products

Primary Antibodies

Complementary Products

Primary Antibodies

Assay Kits

Primary Antibodies

Primary Antibodies

Product promise