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AB221901

Anti-PIAS3 antibody

4

(1 Review)

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(1 Publication)

Rabbit Polyclonal PIAS3 antibody. Suitable for ICC/IF and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human PIAS3 aa 500 to C-terminus.

View Alternative Names

E3 SUMO-protein ligase PIAS3, E3 SUMO-protein transferase PIAS3, Protein inhibitor of activated STAT protein 3, PIAS3

1 Images
Immunocytochemistry/ Immunofluorescence - Anti-PIAS3 antibody (AB221901)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-PIAS3 antibody (AB221901)

PFA-fixed, Triton X-100 permeabilized MCF7 (human breast adenocarcinoma cell line) cells stained for PIAS3 (green) using ab221901 at 4 μg/ml in ICC/IF.

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

ICC/IF

applications

Immunogen

Recombinant Fragment Protein within Human PIAS3 aa 500 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

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Primary Antibodies

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "0.25-2 µg/mL", "ICCIF-species-notes": "<p>Fixation/Permeabilization: PFA/Triton X-100.</p>" }, "Mouse": { "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7.2 Preservative: 0.02% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine)
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

PIAS3 also known as Protein Inhibitor of Activated STAT3 plays a role in regulating transcription by modulating the activity of signal transducer and activator of transcription (STAT) proteins particularly STAT3. PIAS3 exerts its function through SUMOylation a post-translational modification that alters the function of target proteins. This protein has a mass of approximately 67 kDa and is widely expressed in various tissues including the brain lung and spleen.
Biological function summary

PIAS3 influences cellular processes such as cell growth survival and differentiation through its interaction with STAT3. It is part of a multiprotein complex involving several transcription factors that influence gene expression. By modifying STAT3 activity PIAS3 plays an essential role in maintaining cellular homeostasis and responding to extracellular stimuli.

Pathways

PIAS3 directly affects the JAK-STAT signaling cascade a pathway important in transmitting information from chemical signals outside the cell to the cell nucleus resulting in DNA transcription. It interacts predominantly with the STAT family of proteins including STAT1 impacting processes like immune regulation. PIAS3's role in this pathway positions it as a significant modulator of cytokine signaling and immune responses.

Abnormal PIAS3 function has links to cancer and autoimmune diseases. In cancer overactive STAT3 signaling often resulting from impaired PIAS3 function contributes to tumorigenesis. In autoimmune disorders PIAS3 dysregulation may exacerbate conditions due to its role in immune signaling. Connections between PIAS3 and proteins such as STAT3 highlight its disease relevance and offer potential therapeutic targets for managing these conditions.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Functions as an E3-type small ubiquitin-like modifier (SUMO) ligase, stabilizing the interaction between UBE2I and the substrate, and as a SUMO-tethering factor. Plays a crucial role as a transcriptional coregulation in various cellular pathways, including the STAT pathway and the steroid hormone signaling pathway. Involved in regulating STAT3 signaling via inhibiting STAT3 DNA-binding and suppressing cell growth. Enhances the sumoylation of MTA1 and may participate in its paralog-selective sumoylation (PubMed : 21965678, PubMed : 9388184). Sumoylates CCAR2 which promotes its interaction with SIRT1 (PubMed : 25406032). Diminishes the sumoylation of ZFHX3 by preventing the colocalization of ZFHX3 with SUMO1 in the nucleus (PubMed : 24651376).
See full target information PIAS3
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Journal of oncology 2022:2378253 PubMed36090903

2022

CKAP2L Promotes Esophageal Squamous Cell Carcinoma Progression and Drug-Resistance by Modulating Cell Cycle.

Applications

Unspecified application

Species

Unspecified reactive species

Wenhu Chen,Yu Wang,Lifang Wang,Hongguang Zhao,Xiaoyan Li
View all publications
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Product promise

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