AB151491

Anti-PIBF antibody

Name: Anti-PIBF antibody (ab151491) | Abcam
Brand: Abcam Limited
SKU: AB151491

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(1 Publication)

Rabbit Polyclonal PIBF antibody. Suitable for WB, ICC/IF and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human PIBF1 aa 450-700.

View Alternative Names

C13orf24, PIBF, PIBF1, Progesterone-induced-blocking factor 1, Centrosomal protein of 90 kDa, CEP90

2 Images

Immunocytochemistry/ Immunofluorescence - Anti-PIBF antibody (AB151491)

ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-PIBF antibody (AB151491)

Immunofluorescence analysis of methanol fixed HCT116 cell labeling PIBF using ab151491 at 1/500 dilution. The image in the lower panel was co-stained with Hoechst 33342.

Unknown

Western blot - Anti-PIBF antibody (AB151491)

7.5% SDS PAGE

All lanes:

Western blot - Anti-PIBF antibody (ab151491) at 1/1000 dilution

All lanes:

HCT116 whole cell lysate at 30 µg

Predicted band size: 55 kDa,72 kDa,76 kDa,82 kDa,90 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

WB, ICC/IF

applications

Immunogen

Recombinant Fragment Protein within Human PIBF1 aa 450-700. The exact immunogen used to generate this antibody is proprietary information.

Q8WXW3

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100 - 1/1000", "ICCIF-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7 Preservative: 0.01% Thimerosal (merthiolate) Constituents: PBS, 20% Glycerol (glycerin, glycerine), 1% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The progesterone-induced blocking factor (PIBF) is a protein with a molecular mass of approximately 90 kDa. It has important roles linked to cell division and immune response regulation. PIBF is produced in larger quantities in pregnancy tissues particularly by decidual and trophoblastic cells but normal lymphocytes also express it when stimulated by progesterone. Researchers often study its activity because of its potential relevance in pregnancy and immune system interactions.

Biological function summary

Researchers already documented that PIBF plays a role in modulating immune responses during pregnancy. It acts as part of a complex involving progesterone and cytokines working to prevent the mother's immune system from rejecting the fetus. PIBF achieves this by influencing the Th1/Th2 cytokine balance increasing anti-inflammatory cytokines and decreasing pro-inflammatory cytokines like IL-6 and IL-12. This helps to maintain a tolerogenic environment during pregnancy.

Pathways

The PIBF significantly affects pathways involving immune regulation and inflammation. It interacts with the hormonal pathway of progesterone linking it to the immune-modulatory functions controlled by cytokine action. The balance between these pathways ensures immune tolerance in the placenta. PIBF also shares a functional pathway with proteins such as interleukin-10 (IL-10) as they both modulate immune responses to support successful pregnancies.

PIBF has significant associations with pregnancy-related conditions and certain cancers. Abnormal PIBF levels may relate to pregnancy complications such as recurrent spontaneous abortion where immune tolerance gets disrupted. Additionally PIBF can impact tumor growth because it promotes an immunosuppressive environment similar to that observed in pregnancy potentially benefiting malignant cells. This connection suggests that PIBF along with related proteins like IL-10 could serve as a target for therapeutic interventions in these conditions.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
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Target data

Plays a role in ciliogenesis.. Isoform 1. Pericentriolar protein required to maintain mitotic spindle pole integrity (PubMed : 21224392). Required for the centrosomal accumulation of PCM1 and the recruitment of centriolar satellite proteins such as BBS4. Via association with PCM1 may be involved in primary cilia formation (PubMed : 23110211). Required for CEP63 centrosomal localization and its interaction with WDR62. Together with CEP63 promotes centriole duplication. Promotes the centrosomal localization of CDK2 (PubMed : 26297806).. Isoform 4. The secreted form is a mediator of progesterone that by acting on the phospholipase A2 enzyme interferes with arachidonic acid metabolism, induces a Th2 biased immune response, and by controlling decidual natural killer cells (NK) activity exerts an anti-abortive effect (PubMed : 12516630, PubMed : 14634107, PubMed : 3863495). Increases the production of Th2-type cytokines by signaling via the JAK/STAT pathway. Activates STAT6 and inhibits STAT4 phosphorylation. Signaling via a not identified receptor seems to implicate IL4R and a GPI-anchored protein (PubMed : 16393965, PubMed : 25218441).

See full target information PIBF1

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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Investigative ophthalmology & visual science 65:15 PubMed39504050

2024

Cysteine Leukotriene Receptor Antagonist-Montelukast Effects on Diabetic Retinal Microvascular Endothelial Cells Curtail Autophagy.

Applications

Unspecified application

Species

Unspecified reactive species

Ahmed M Awad,Amritha T M Seetharaman,Mohammad Shahadat Hossain,Sally L Elshaer,Rania R Abdelaziz,Manar A Nader,Rajashekhar Gangaraju

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-PIBF antibody

Immunocytochemistry/ Immunofluorescence - Anti-PIBF antibody (AB151491)

Western blot - Anti-PIBF antibody (AB151491)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (1)

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