Rabbit Polyclonal PICH antibody. Suitable for IHC-P and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human ERCC6L.
Images
Publications
Filter by
- Scientific reports 11:62552021Adipose most abundant 2 protein is a predictive marker for cisplatin sensitivity in cancers.Applications:Unspecified applicationReactive species:Unspecified reactive speciesKenya Kamimura,Takeshi Suda,Yasuo Fukuhara,Shujiro Okuda,Yu Watanabe,Takeshi Yokoo,Akihiko Osaki,Nobuo Waguri,Toru Ishikawa,Toshihiro Sato,Yutaka Aoyagi,Masaaki Takamura,Toshifumi Wakai,Shuji TeraiPubMed 33737617
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.4
Preservative: 0.05% Sodium azide
Constituents: 50% Glycerol (glycerin, glycerine), 49% PBS- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Recombinant Fragment Protein within Human ERCC6L. The exact immunogen used to generate this antibody is proprietary information. Database link Q2NKX8
Reactivity data
Select an application| IHC-P | |
|---|---|
| Human | Tested |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/50.00000 - 1/200.00000 | Notes - |
Associated Products
Select an associated product type
1 product for Alternative Product
Target data
Function
DNA helicase that acts as a tension sensor that associates with catenated DNA which is stretched under tension until it is resolved during anaphase (PubMed:17218258, PubMed:23973328). Functions as ATP-dependent DNA translocase (PubMed:23973328, PubMed:28977671). Can promote Holliday junction branch migration (in vitro) (PubMed:23973328).
Alternative names
PICH, ERCC6L, DNA excision repair protein ERCC-6-like, ATP-dependent helicase ERCC6-like, PLK1-interacting checkpoint helicase, Tumor antigen BJ-HCC-15
Recommended products
Rabbit Polyclonal PICH antibody. Suitable for IHC-P and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human ERCC6L.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.4
Preservative: 0.05% Sodium azide
Constituents: 50% Glycerol (glycerin, glycerine), 49% PBS- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Recombinant Fragment Protein within Human ERCC6L. The exact immunogen used to generate this antibody is proprietary information. Database link Q2NKX8
- Purification technique
- Affinity purification Immunogen
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.
Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
The protein PICH also known as Plk1-interacting checkpoint helicase is an important factor in maintaining chromosome integrity during cell division. PICH is a helicase with a molecular mass of approximately 135 kDa. This protein is mainly expressed in tissues with a high rate of cell division. It possesses ATPase activity which allows it to unwind DNA structures playing a mechanical role in chromosome segregation. The helicase activity of PICH is necessary in mitosis where it interacts with centromeres during spindle assembly checkpoint activation.
- Biological function summary
PICH contributes to the resolution of ultra-fine DNA bridges which occur during the late stages of mitosis. These DNA bridges can compromise genome stability leading to genetic disorders if not properly resolved. PICH works with other proteins like topoisomerase II and Bloom syndrome protein (BLM) to manage these structures. This activity suggests PICH is part of a complex necessary for protecting chromosomal stability during cell division ensuring accurate segregation of sister chromatids.
- Pathways
PICH plays a role in the spindle assembly checkpoint (SAC) and mitotic exit pathway. Within these pathways PICH interacts with proteins such as Polo-like kinase 1 (Plk1) and Aurora B both important for the regulation of mitosis. PICH's interaction with these proteins highlights its significance in controlling the proper timing and execution of chromosome separation which is essential for correct mitotic progression and prevention of aneuploidy.
- Associated diseases and disorders
PICH is associated with conditions that involve genomic instability like cancer. Aberrations in PICH function can result in incorrect chromosome segregation contributing to tumorigenesis. Additionally PICH is linked with disorders involving defective DNA repair mechanisms such as those involving BRCA1 a protein important for repairing DNA double-strand breaks. Dysfunctional PICH leads to enhanced chromosomal aberrations highlighting its relevance in maintaining genomic integrity and linking it to significant pathological states.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
2 product images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PICH antibody (ab197925)
Immunohistochemical analysis of paraffin-embedded Human colon cancer tissue labeling PICH using ab197925 at 1/30 dilution.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PICH antibody (ab197925)
Immunohistochemical analysis of paraffin-embedded Human cervical cancer tissue labeling PICH using ab197925 at 1/30 dilution.
Downloads
Product protocols
- Visit the general protocols
- Visit troubleshooting
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
For licensing inquiries, please contact partnerships@abcam.com