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AB88560

Anti-PICH antibody

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(3 Publications)

Mouse Polyclonal PICH antibody. Carrier free. Suitable for WB and reacts with Human samples. Cited in 3 publications. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human ERCC6L.

View Alternative Names

PICH, ERCC6L, DNA excision repair protein ERCC-6-like, ATP-dependent helicase ERCC6-like, PLK1-interacting checkpoint helicase, Tumor antigen BJ-HCC-15

2 Images
Western blot - Anti-PICH antibody (AB88560)
  • WB

Unknown

Western blot - Anti-PICH antibody (AB88560)

All lanes:

Western blot - Anti-PICH antibody (ab88560) at 1 µg/mL

All lanes:

Hela cell lysate at 50 µg

Predicted band size: 141 kDa

Observed band size: 141 kDa,57 kDa

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Western blot - Anti-PICH antibody (AB88560)
  • WB

Unknown

Western blot - Anti-PICH antibody (AB88560)

All lanes:

Western blot - Anti-PICH antibody (ab88560) at 1 µg/mL

Lane 1:

PICH transfected 293T cell lysate at 25 µg

Lane 2:

Non transfected 293T cell lysate at 25 µg

Predicted band size: 141 kDa

Observed band size: 60 kDa

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Key facts

Host species

Mouse

Clonality

Polyclonal

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

WB

applications

Immunogen

Recombinant Full Length Protein corresponding to Human ERCC6L.

Q2NKX8

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Reactivity data

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The protein PICH also known as Plk1-interacting checkpoint helicase is an important factor in maintaining chromosome integrity during cell division. PICH is a helicase with a molecular mass of approximately 135 kDa. This protein is mainly expressed in tissues with a high rate of cell division. It possesses ATPase activity which allows it to unwind DNA structures playing a mechanical role in chromosome segregation. The helicase activity of PICH is necessary in mitosis where it interacts with centromeres during spindle assembly checkpoint activation.
Biological function summary

PICH contributes to the resolution of ultra-fine DNA bridges which occur during the late stages of mitosis. These DNA bridges can compromise genome stability leading to genetic disorders if not properly resolved. PICH works with other proteins like topoisomerase II and Bloom syndrome protein (BLM) to manage these structures. This activity suggests PICH is part of a complex necessary for protecting chromosomal stability during cell division ensuring accurate segregation of sister chromatids.

Pathways

PICH plays a role in the spindle assembly checkpoint (SAC) and mitotic exit pathway. Within these pathways PICH interacts with proteins such as Polo-like kinase 1 (Plk1) and Aurora B both important for the regulation of mitosis. PICH's interaction with these proteins highlights its significance in controlling the proper timing and execution of chromosome separation which is essential for correct mitotic progression and prevention of aneuploidy.

PICH is associated with conditions that involve genomic instability like cancer. Aberrations in PICH function can result in incorrect chromosome segregation contributing to tumorigenesis. Additionally PICH is linked with disorders involving defective DNA repair mechanisms such as those involving BRCA1 a protein important for repairing DNA double-strand breaks. Dysfunctional PICH leads to enhanced chromosomal aberrations highlighting its relevance in maintaining genomic integrity and linking it to significant pathological states.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

DNA helicase that acts as a tension sensor that associates with catenated DNA which is stretched under tension until it is resolved during anaphase (PubMed : 17218258, PubMed : 23973328). Functions as ATP-dependent DNA translocase (PubMed : 23973328, PubMed : 28977671). Can promote Holliday junction branch migration (in vitro) (PubMed : 23973328).
See full target information ERCC6L
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Publications (3)

Recent publications for all applications. Explore the full list and refine your search

Epigenomes 6: PubMed36278682

2022

PLK-1 Interacting Checkpoint Helicase, PICH, Mediates Cellular Oxidative Stress Response.

Applications

Unspecified application

Species

Unspecified reactive species

Anindita Dutta,Apurba Das,Deepa Bisht,Vijendra Arya,Rohini Muthuswami

Molecular cancer 18:151 PubMed31672157

2019

Circ-AKT3 inhibits clear cell renal cell carcinoma metastasis via altering miR-296-3p/E-cadherin signals.

Applications

Unspecified application

Species

Unspecified reactive species

Dingwei Xue,Huan Wang,Yuanlei Chen,Danyang Shen,Jieyang Lu,Mingchao Wang,Abudureheman Zebibula,Liwei Xu,Haiyang Wu,Gonghui Li,Liqun Xia

Molecular cell 76:27-43.e11 PubMed31447390

2019

SLX4IP Antagonizes Promiscuous BLM Activity during ALT Maintenance.

Applications

Unspecified application

Species

Unspecified reactive species

Stephanie Panier,Marija Maric,Graeme Hewitt,Emily Mason-Osann,Himabindu Gali,Anqi Dai,Adam Labadorf,Jean-Hugues Guervilly,Philip Ruis,Sandra Segura-Bayona,Ondrej Belan,Paulina Marzec,Pierre-Henri L Gaillard,Rachel L Flynn,Simon J Boulton
View all publications
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