Anti-PICK1 antibody [EPR4130(3)] - BSA and Azide free

• WB

Lab

Western blot - Anti-PICK1 antibody [EPR4130(3)] - BSA and Azide free (AB248652)

This data was developed using ab133773, the same antibody clone in a different buffer formulation.

Western blot : Rabbit Monoclonal[EPR4130(3)] to PICK1 ab133773 staining at 1/1000 dilution, shown in green; Mouse anti GAPDH (ab8245) loading control staining at 1/20,000 dilution, shown in magenta. A band was observed at 47 kDa in Wild-type U-87 MG cell lysates with no signal observed at this size in PICK1 knockout U-87 MG cell line (ab326021). To generate this image, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 5pc Milk in TBS-0.1 % Tween^® 20 (TBS-T) before incubation with primary antibodies overnight at 4°C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Goat anti-Rabbit 800CW & Goat anti-Mouse 680RD at 1/20,000 dilution.

All lanes:

Western blot - Anti-PICK1 antibody [EPR4130(3)] (<a href='/en-us/products/primary-antibodies/pick1-antibody-epr41303-ab133773'>ab133773</a>) at 1/1000 dilution

Lane 1:

Wild-type U-87 MG at 20 µg

Lane 2:

Western blot - Human PICK1 knockout U-87 MG cell line (<a href='/en-us/products/cell-lines/human-pick1-knockout-u-87-mg-cell-line-ab326021'>ab326021</a>) at 20 µg

Lane 2:

PICK1 knockout U-87 MG at 20 µg

Lane 3:

T-47D at 20 µg

Lane 4:

MCF7 at 20 µg

Secondary

All lanes:

Goat anti-Rabbit 800CW & Goat anti-Mouse 680RD at 1/20000 dilution

Predicted band size: 47 kDa

Observed band size: 47 kDa

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• WB

Unknown

Western blot - Anti-PICK1 antibody [EPR4130(3)] - BSA and Azide free (AB248652)

This data was developed using ab133773, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-PICK1 antibody [EPR4130(3)] (<a href='/en-us/products/primary-antibodies/pick1-antibody-epr41303-ab133773'>ab133773</a>) at 1/1000 dilution

Lane 1:

Human fetal brain lysate at 10 µg

Lane 2:

LnCaP lysate at 10 µg

Secondary

All lanes:

HRP labelled Goat anti Rabbit IgG at 1/2000 dilution

Predicted band size: 46 kDa

Observed band size: 50 kDa

false

• OI-RD Scanning

Unknown

OI-RD Scanning - Anti-PICK1 antibody [EPR4130(3)] - BSA and Azide free (AB248652)

We have systematically measured KD (the equilibrium dissociation constant between the antibody and its antigen), of more than 840 recombinant antibodies to assess not only their individual KD values but also to see the average affinity of antibody. Based on the comparison with published literature values for mouse monoclonal antibodies, Recombinant antibodies appear to be on average 1-2 order of magnitude higher affinity.