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Rabbit Recombinant Monoclonal PICK1 antibody. Carrier free. Suitable for WB and reacts with Human samples.

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Images

Western blot - Anti-PICK1 antibody [EPR4130(3)] - BSA and Azide free (AB248652), expandable thumbnail
  • OI-RD Scanning - Anti-PICK1 antibody [EPR4130(3)] - BSA and Azide free (AB248652), expandable thumbnail
  • Western blot - Anti-PICK1 antibody [EPR4130(3)] - BSA and Azide free (AB248652), expandable thumbnail

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7.2 - 7.4
Constituents: PBS

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
ICC/IFFlow CytWBIHC-P
Human
Not recommended
Not recommended
Tested
Not recommended

Not recommended
Not recommended

Species
Human
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Human
Dilution info
-
Notes

-

Tested
Tested

Species
Human
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Human
Dilution info
-
Notes

-

Associated Products

Select an associated product type

2 products for Alternative Product

Target data

Function

Probable adapter protein that bind to and organize the subcellular localization of a variety of membrane proteins containing some PDZ recognition sequence. Involved in the clustering of various receptors, possibly by acting at the receptor internalization level. Plays a role in synaptic plasticity by regulating the trafficking and internalization of AMPA receptors. May be regulated upon PRKCA activation. May regulate ASIC1/ASIC3 channel. Regulates actin polymerization by inhibiting the actin-nucleating activity of the Arp2/3 complex; the function is competitive with nucleation promoting factors and is linked to neuronal morphology regulation and AMPA receptor (AMPAR) endocytosis. Via interaction with the Arp2/3 complex involved in regulation of synaptic plasicity of excitatory synapses and required for spine shrinkage during long-term depression (LTD). Involved in regulation of astrocyte morphology, antagonistic to Arp2/3 complex activator WASL/N-WASP function.

Alternative names

PRKCABP, PICK1, PRKCA-binding protein, Protein interacting with C kinase 1, Protein kinase C-alpha-binding protein

Recommended products

Rabbit Recombinant Monoclonal PICK1 antibody. Carrier free. Suitable for WB and reacts with Human samples.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Carrier free
Yes
Clone number
EPR4130(3)
Purification technique
Affinity purification
Dissociation constant
5.3 x 10-11 M
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze

Notes

ab248652 is the carrier-free version of Anti-PICK1 antibody [EPR4130(3)] ab133773.

Species reactivity
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species.
Please contact us for more information.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

PICK1 or Protein Interacting with C Kinase 1 is a protein that plays a significant role in cellular processes. With a molecular mass of approximately 47 kDa PICK1 is well known for its ability to bind to proteins through its PDZ domain. You can find PICK1 expressed highly in the brain but it is also present in other tissues like the pancreas and testes. This makes it an important target for understanding various cellular interactions and functions related to protein trafficking.

Biological function summary

PICK1 is known for its role in modulating the trafficking and synaptic targeting of AMPA receptors which are essential for synaptic plasticity and memory formation. It operates as part of a protein complex that influences the receptor's presence at the synaptic membrane. PICK1 binds with proteins like GluA2 subunit of AMPA receptors which allows it to regulate receptor internalization and surface expression impacting synaptic signaling and plasticity.

Pathways

PICK1 interacts within the glutamatergic signaling and insulin secretion pathways. In the glutamatergic signaling pathway it contributes to the regulation of synaptic strength by linking with other proteins such as protein kinase C (PKC) to facilitate synapse dynamics. In insulin secretion PICK1 partners with proteins like the insulin granule docking machinery to regulate insulin release in response to glucose.

Associated diseases and disorders

Research has linked PICK1 function with conditions like schizophrenia and diabetes. PICK1's regulatory influence on AMPA receptors involves interactions with proteins such as protein kinase C which researchers have associated with synaptic abnormalities seen in schizophrenia. Regarding diabetes PICK1's role in insulin secretion connects it to the disease through the regulation of proteins involved in insulin granule dynamics. Understanding these connections aids in developing potential therapeutic strategies for these disorders.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

3 product images

  • Western blot - Anti-PICK1 antibody [EPR4130(3)] - BSA and Azide free (ab248652), expandable thumbnail

    Western blot - Anti-PICK1 antibody [EPR4130(3)] - BSA and Azide free (ab248652)

    This data was developed using Anti-PICK1 antibody [EPR4130(3)] ab133773, the same antibody clone in a different buffer formulation.

    All lanes: Western blot - Anti-PICK1 antibody [EPR4130(3)] (Anti-PICK1 antibody [EPR4130(3)] ab133773) at 1/1000 dilution

    Lane 1: Human fetal brain lysate at 10 µg

    Lane 2: LnCaP lysate at 10 µg

    Secondary

    All lanes: HRP labelled Goat anti Rabbit IgG at 1/2000 dilution

    Predicted band size: 46 kDa

    Observed band size: 50 kDa

  • OI-RD Scanning - Anti-PICK1 antibody [EPR4130(3)] - BSA and Azide free (ab248652), expandable thumbnail

    OI-RD Scanning - Anti-PICK1 antibody [EPR4130(3)] - BSA and Azide free (ab248652)

    We have systematically measured KD (the equilibrium dissociation constant between the antibody and its antigen), of more than 840 recombinant antibodies to assess not only their individual KD values but also to see the average affinity of antibody.
    Based on the comparison with published literature values for mouse monoclonal antibodies, Recombinant antibodies appear to be on average 1-2 order of magnitude higher affinity.

  • Western blot - Anti-PICK1 antibody [EPR4130(3)] - BSA and Azide free (ab248652), expandable thumbnail

    Western blot - Anti-PICK1 antibody [EPR4130(3)] - BSA and Azide free (ab248652)

    PICK1 Western blot staining using rabbit Anti-PICK1 antibody

    This data was developed using Anti-PICK1 antibody [EPR4130(3)] ab133773, the same antibody clone in a different buffer formulation.

    Western blot: Rabbit Monoclonal[EPR4130(3)] to PICK1 Anti-PICK1 antibody [EPR4130(3)] ab133773 staining at 1/1000 dilution, shown in green; Mouse anti GAPDH (Anti-GAPDH antibody [6C5] - Loading Control ab8245) loading control staining at 1/20,000 dilution, shown in magenta. A band was observed at 47 kDa in Wild-type U-87 MG cell lysates with no signal observed at this size in PICK1 knockout U-87 MG cell line. To generate this image, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 5pc Milk in TBS-0.1 % Tween® 20 (TBS-T) before incubation with primary antibodies overnight at 4°C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Goat anti-Rabbit 800CW & Goat anti-Mouse 680RD at 1/20,000 dilution.

    All lanes: Western blot - Anti-PICK1 antibody [EPR4130(3)] (Anti-PICK1 antibody [EPR4130(3)] ab133773) at 1/1000 dilution

    Lane 1: Wild-type U-87 MG at 20 µg

    Lane 2: PICK1 knockout U-87 MG at 20 µg

    Lane 3: T-47D at 20 µg

    Lane 4: MCF7 at 20 µg

    Secondary

    All lanes: Goat anti-Rabbit 800CW & Goat anti-Mouse 680RD at 1/20000 dilution

    Performed under reducing conditions.

    Predicted band size: 47 kDa

    Observed band size: 47 kDa

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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