AB252543

Anti-PICK1 antibody [L20/8]

Recombinant
KO Validated
What is this?

Be the first to review this product! Submit a review

(2 Publications)

Mouse Recombinant Monoclonal PICK1 antibody. Suitable for WB and reacts with Mouse, Rat, Human samples. Cited in 2 publications.

View Alternative Names

PRKCABP, PICK1, PRKCA-binding protein, Protein interacting with C kinase 1, Protein kinase C-alpha-binding protein

3 Images

Lab

Western blot - Anti-PICK1 antibody [L20/8] (AB252543)

Western blot : Mouse Monoclonal[L20/8] to PICK1 ab252543 staining at 0.5 µg/mL, shown in green; Rabbit anti GAPDH (ab181602) loading control staining at 1/20,000 dilution, shown in magenta. A band was observed at 50 kDa in Wild-type U-87 MG cell lysates with no signal observed at this size in PICK1 knockout U-87 MG cell line (ab326021). To generate this image, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 3pc Milk in TBS-0.1 % Tween^® 20 (TBS-T) before incubation with primary antibodies overnight at 4°C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Goat anti-Mouse 800CW & Goat anti-Rabbit 680RD at 1/20,000 dilution.

All lanes:

Western blot - Anti-PICK1 antibody [L20/8] (ab252543) at 0.5 µg/mL

Lane 1:

Wild-type U-87 MG at 20 µg

Lane 2:

Western blot - Human PICK1 knockout U-87 MG cell line (<a href='/en-us/products/cell-lines/human-pick1-knockout-u-87-mg-cell-line-ab326021'>ab326021</a>) at 20 µg

Lane 2:

PICK1 knockout U-87 MG at 20 µg

Lane 3:

MCF7 at 20 µg

Secondary

All lanes:

Goat anti-Mouse 800CW & Goat anti-Rabbit 680RD at 1/20000 dilution

Predicted band size: 47 kDa

Observed band size: 50 kDa

false

Supplier Data

Western blot - Anti-PICK1 antibody [L20/8] (AB252543)

Blocking/Dilution buffer : 5% NFDM/TBST.

This blot was developed using a higher sensitivity ECL substrate.

All lanes:

Western blot - Anti-PICK1 antibody [L20/8] (ab252543) at 0.509 µg/mL

All lanes:

HeLa (human cervix adenocarcinoma epithelial cell) whole cell lysate at 20 µg

Secondary

All lanes:

Peroxidase-Conjugated Goat anti-Mouse IgG (H+L) at 1/10000 dilution

Predicted band size: 46 kDa

false

Exposure time: 3min

Supplier Data

Western blot - Anti-PICK1 antibody [L20/8] (AB252543)

This image was produced using the same antibody clone but in a different formulation ab252543, PBS, sodium azide, glycerol and BSA.

Blocking/Dilution buffer : 5% NFDM/TBST.

All lanes:

Western blot - Anti-PICK1 antibody [L20/8] (ab252543) at 0.509 µg/mL

Lane 1:

Mouse brain tissue lysate at 20 µg

Lane 2:

Rat brain tissue lysate at 20 µg

Secondary

All lanes:

Peroxidase-Conjugated Goat anti-Mouse IgG (H+L) at 1/10000 dilution

Predicted band size: 46 kDa

false

Exposure time: 15s

Carrier free

Anti-PICK1 antibody [L20/8] - BSA and Azide free

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

L20/8

Isotype

IgG2a

Carrier free

Reacts with

Mouse, Rat, Human

Applications

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

style

left-column

style

left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.5 µg/mL", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Mouse": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.509 µg/mL", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.509 µg/mL", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

style

left-column

Product details

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Want a custom formulation?
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com

style

left-column

Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

style

left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

PICK1 or Protein Interacting with C Kinase 1 is a protein that plays a significant role in cellular processes. With a molecular mass of approximately 47 kDa PICK1 is well known for its ability to bind to proteins through its PDZ domain. You can find PICK1 expressed highly in the brain but it is also present in other tissues like the pancreas and testes. This makes it an important target for understanding various cellular interactions and functions related to protein trafficking.

Biological function summary

PICK1 is known for its role in modulating the trafficking and synaptic targeting of AMPA receptors which are essential for synaptic plasticity and memory formation. It operates as part of a protein complex that influences the receptor's presence at the synaptic membrane. PICK1 binds with proteins like GluA2 subunit of AMPA receptors which allows it to regulate receptor internalization and surface expression impacting synaptic signaling and plasticity.

Pathways

PICK1 interacts within the glutamatergic signaling and insulin secretion pathways. In the glutamatergic signaling pathway it contributes to the regulation of synaptic strength by linking with other proteins such as protein kinase C (PKC) to facilitate synapse dynamics. In insulin secretion PICK1 partners with proteins like the insulin granule docking machinery to regulate insulin release in response to glucose.

Research has linked PICK1 function with conditions like schizophrenia and diabetes. PICK1's regulatory influence on AMPA receptors involves interactions with proteins such as protein kinase C which researchers have associated with synaptic abnormalities seen in schizophrenia. Regarding diabetes PICK1's role in insulin secretion connects it to the disease through the regulation of proteins involved in insulin granule dynamics. Understanding these connections aids in developing potential therapeutic strategies for these disorders.

style

left-column

style

left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

style

left-column

Target data

Probable adapter protein that bind to and organize the subcellular localization of a variety of membrane proteins containing some PDZ recognition sequence. Involved in the clustering of various receptors, possibly by acting at the receptor internalization level. Plays a role in synaptic plasticity by regulating the trafficking and internalization of AMPA receptors. May be regulated upon PRKCA activation. May regulate ASIC1/ASIC3 channel. Regulates actin polymerization by inhibiting the actin-nucleating activity of the Arp2/3 complex; the function is competitive with nucleation promoting factors and is linked to neuronal morphology regulation and AMPA receptor (AMPAR) endocytosis. Via interaction with the Arp2/3 complex involved in regulation of synaptic plasicity of excitatory synapses and required for spine shrinkage during long-term depression (LTD). Involved in regulation of astrocyte morphology, antagonistic to Arp2/3 complex activator WASL/N-WASP function.

See full target information PICK1

style

left-column

Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Signal transduction and targeted therapy 8:219 PubMed37271769

2023

CNTNAP2 intracellular domain (CICD) generated by γ-secretase cleavage improves autism-related behaviors.

Applications

Unspecified application

Species

Unspecified reactive species

Jing Zhang,Fang Cai,Renbin Lu,Xiaoliang Xing,Lu Xu,Kunyang Wu,Zishan Gong,Qing Zhang,Yun Zhang,Mengen Xing,Weihong Song,Jia-Da Li

Brain and behavior 11:e2380 PubMed34582111

2021

FSC231 alleviates paclitaxel-induced neuralgia by inhibiting the interactions between PICK1 and GluA2 and activates GSK-3β and ERK1/2.

Applications

Unspecified application

Species

Unspecified reactive species

Xi Zhang,Jiagao Wang,Ran Ran,Yuchuan Peng,Yun Xiao

View all publications

style

left-column

style

left-column

style

right-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-PICK1 antibody [L20/8]

Western blot - Anti-PICK1 antibody [L20/8] (AB252543)

Western blot - Anti-PICK1 antibody [L20/8] (AB252543)

Western blot - Anti-PICK1 antibody [L20/8] (AB252543)

Related conjugates and formulations (1)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Alternative Products

Primary Antibodies

Primary Antibodies

Complementary Products

Primary Antibodies

Primary Antibodies

Secondary Antibodies

Primary Antibodies

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (2)

Product promise