Anti-PICK1 antibody [L20/8] - BSA and Azide free

• WB

Lab

Western blot - Anti-PICK1 antibody [L20/8] - BSA and Azide free (AB255765)

This data was developed using ab252543, the same antibody clone in a different buffer formulation.

Western blot : Mouse Monoclonal[L20/8] to PICK1 ab252543 staining at 0.5 µg/mL, shown in green; Rabbit anti GAPDH (ab181602) loading control staining at 1/20,000 dilution, shown in magenta. A band was observed at 50 kDa in Wild-type U-87 MG cell lysates with no signal observed at this size in PICK1 knockout U-87 MG cell line. To generate this image, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 3pc Milk in TBS-0.1 % Tween^® 20 (TBS-T) before incubation with primary antibodies overnight at 4°C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Goat anti-Mouse 800CW & Goat anti-Rabbit 680RD at 1/20,000 dilution.

All lanes:

Western blot - Anti-PICK1 antibody [L20/8] (<a href='/en-us/products/primary-antibodies/pick1-antibody-l20-8-ab252543'>ab252543</a>) at 0.5 µg/mL

Lane 1:

Wild-type U-87 MG at 20 µg

Lane 2:

PICK1 knockout U-87 MG at 20 µg

Lane 3:

MCF7 at 20 µg

Secondary

All lanes:

Goat anti-Mouse 800CW & Goat anti-Rabbit 680RD at 1/20000 dilution

Predicted band size: 47 kDa

Observed band size: 50 kDa

false

• WB

Supplier Data

Western blot - Anti-PICK1 antibody [L20/8] - BSA and Azide free (AB255765)

Blocking/Dilution buffer : 5% NFDM/TBST.

This image was produced using the same antibody clone but in a different formulation containing PBS, sodium azide, glycerol and BSA (ab252543).

All lanes:

Western blot - Anti-PICK1 antibody [L20/8] (<a href='/en-us/products/primary-antibodies/pick1-antibody-l20-8-ab252543'>ab252543</a>) at 0.509 µg/mL

Lane 1:

Mouse brain tissue lysate at 20 µg

Lane 2:

Rat brain tissue lysate at 20 µg

Secondary

All lanes:

Peroxidase-Conjugated Goat anti-Mouse IgG (H+L) at 1/10000 dilution

Predicted band size: 46 kDa

false

Exposure time: 15s

• WB

Supplier Data

Western blot - Anti-PICK1 antibody [L20/8] - BSA and Azide free (AB255765)

Blocking/Dilution buffer : 5% NFDM/TBST.

This blot was developed using a higher sensitivity ECL substrate.

This image was produced using the same antibody clone but in a different formulation containing PBS, sodium azide, glycerol and BSA (ab252543).

All lanes:

Western blot - Anti-PICK1 antibody [L20/8] (<a href='/en-us/products/primary-antibodies/pick1-antibody-l20-8-ab252543'>ab252543</a>) at 0.509 µg/mL

All lanes:

HeLa (human cervix adenocarcinoma epithelial cell) whole cell lysate at 20 µg

Secondary

All lanes:

Peroxidase-Conjugated Goat anti-Mouse IgG (H+L) at 1/10000 dilution

Predicted band size: 46 kDa

false

Exposure time: 3min