Rabbit Polyclonal PICOT antibody. Suitable for IP, WB and reacts with Human, Mouse samples. Cited in 1 publication. Immunogen corresponding to Synthetic Peptide within Human GLRX3 aa 250 to C-terminus.
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Publications
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- Scientific reports 11:215202021Genetic variants in histone modification regions are associated with the prognosis of lung adenocarcinoma.Applications:Unspecified applicationReactive species:Unspecified reactive speciesHyo-Gyoung Kang,Yong Hoon Lee,Shin Yup Lee,Jin Eun Choi,Sook Kyung Do,Mi Jeong Hong,Jang Hyuck Lee,Ji Yun Jeong,Young Woo Do,Eung Bae Lee,Kyung Min Shin,Won Kee Lee,Sun Ha Choi,Hye Won Seo,Seung Soo Yoo,Jaehee Lee,Seung Ick Cha,Chang Ho Kim,Sukki Cho,Sanghoon Jheon,Jae Yong ParkPubMed 34728688
Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
pH: 7 - 8
Preservative: 0.09% Sodium azide
Constituents: Tris citrate/phosphate- Form
Liquid
- Clonality
Polyclonal
Immunogen
- Synthetic Peptide within Human GLRX3 aa 250 to C-terminus. The exact immunogen used to generate this antibody is proprietary information. Database link O76003
Reactivity data
Select an application| IP | WB | |
|---|---|---|
| Human | Tested | Tested |
| Mouse | Expected | Tested |
| Rat | Predicted | Predicted |
| Chimpanzee | Predicted | Predicted |
| Cow | Predicted | Predicted |
| Dog | Predicted | Predicted |
| Orangutan | Predicted | Predicted |
| Pig | Predicted | Predicted |
| Rabbit | Predicted | Predicted |
| Rhesus monkey | Predicted | Predicted |
| Sheep | Predicted | Predicted |
IP
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 2.00000-10.00000 µg/mg of lysate | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info Use at an assay dependent concentration. | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Rat, Sheep, Rabbit, Cow, Dog, Pig, Chimpanzee, Rhesus monkey, Orangutan | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 1/2000.00000 - 1/10000.00000 | Notes - |
Species Human | Dilution info 1/2000.00000 - 1/10000.00000 | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Rat, Sheep, Rabbit, Cow, Dog, Pig, Chimpanzee, Rhesus monkey, Orangutan | Dilution info - | Notes - |
Target data
Function
Together with BOLA2, acts as a cytosolic iron-sulfur (Fe-S) cluster assembly factor that facilitates [2Fe-2S] cluster insertion into a subset of cytosolic proteins (PubMed:26613676, PubMed:27519415). Acts as a critical negative regulator of cardiac hypertrophy and a positive inotropic regulator (By similarity). Required for hemoglobin maturation (PubMed:23615448). Does not possess any thyoredoxin activity since it lacks the conserved motif that is essential for catalytic activity.
Alternative names
Glutaredoxin-3, PKC-interacting cousin of thioredoxin, PKC-theta-interacting protein, Thioredoxin-like protein 2, PICOT, PKCq-interacting protein, GLRX3, PICOT, TXNL2, HUSSY-22
Recommended products
Rabbit Polyclonal PICOT antibody. Suitable for IP, WB and reacts with Human, Mouse samples. Cited in 1 publication. Immunogen corresponding to Synthetic Peptide within Human GLRX3 aa 250 to C-terminus.
Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
pH: 7 - 8
Preservative: 0.09% Sodium azide
Constituents: Tris citrate/phosphate- Form
Liquid
- Clonality
Polyclonal
- Immunogen
- Synthetic Peptide within Human GLRX3 aa 250 to C-terminus. The exact immunogen used to generate this antibody is proprietary information. Database link O76003
- Purification technique
Affinity purification Immunogen
- Concentration
- Purification notes
ab226396 was affinity purified using an epitope specific to PICOT immobilized on solid support.
Storage
- Shipped at conditions
Blue Ice
- Appropriate short-term storage duration
1-2 weeks
- Appropriate short-term storage conditions
+4°C
- Appropriate long-term storage conditions
-20°C
- Aliquoting information
Upon delivery aliquot
- Storage information
Avoid freeze / thaw cycle
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.
Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
Supplementary info
This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
PICOT also known as thioredoxin-interacting protein (TXNIP) or Vitamin D3 up-regulated protein 1 (VDUP1) is a protein with a mass of approximately 39 kDa. It interacts with several cellular components including thioredoxin acting as a regulatory inhibitor. PICOT is expressed in a variety of tissues with notable presence in heart and muscle tissues. It influences the redox state of the cell playing a significant role in oxidative stress response.
- Biological function summary
PICOT acts as a regulator of the thiol redox system in cells. It forms a complex with thioredoxin thereby inhibiting its activity which leads to an accumulation of oxidized proteins. This activity makes PICOT an important player in redox signaling affecting cellular responses to oxidative stress. Through its interactions PICOT contributes to cellular processes such as apoptosis differentiation and proliferation mediated by redox changes.
- Pathways
PICOT is integral to the cellular oxidative stress response and redox regulation pathways. It interacts with proteins like thioredoxin and possibly influences NADPH oxidase activity. By modulating thioredoxin PICOT impacts the NF-kB signaling pathway which plays a role in inflammation and immune responses. These pathways highlight PICOT's involvement in maintaining cellular homeostasis especially in stress conditions.
- Associated diseases and disorders
PICOT has links to diabetes and cardiovascular disorders. It affects glucose metabolism and insulin signaling connecting to proteins such as insulin receptor substrates. PICOT also involves in heart disorders given its role in cardiac muscle stress responses and connection to proteins like troponin. Understanding these relationships can aid in developing therapeutic approaches for managing oxidative stress-related diseases.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
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2 product images
Western blot - Anti-PICOT antibody (ab226396)
Lysates prepared using NETN lysis buffer.
All lanes: Western blot - Anti-PICOT antibody (ab226396) at 0.1 µg/mL
Lane 1: HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 50 µg
Lane 2: HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate at 50 µg
Lane 3: Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate at 50 µg
Lane 4: TCMK-1 (mouse kidney epithelial cell line) whole cell lysate at 50 µg
Lane 5: NIH/3T3 (mouse embryo fibroblast cell line) whole cell lysate at 50 µg
Developed using the ECL technique.
Predicted band size: 37 kDa
Exposure time: 10s
Immunoprecipitation - Anti-PICOT antibody (ab226396)
PICOT was immunoprecipitated from HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate (prepared using NETN lysis buffer; 1 mg for IP, 20% of IP loaded) with ab226396 at 6 μg/mg lysate. Western blot was performed from the immunoprecipitate using ab226396 at 0.4 μg/ml.
Lane 1: ab226396 IP in HEK-293T whole cell lysate.
Lane 2: Control IgG IP in HEK-293T whole cell lysate.
Detection: Chemiluminescence with exposure time of 10 seconds.
All lanes: Immunoprecipitation - Anti-PICOT antibody (ab226396)
Predicted band size: 37 kDa
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