AB226396

Anti-PICOT antibody

Name: Anti-PICOT antibody (ab226396) | Abcam
Brand: Abcam Limited
SKU: AB226396
Price: 525 USD
Availability: InStock

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(2 Publications)

Rabbit Polyclonal PICOT antibody. Suitable for IP, WB and reacts with Human, Mouse samples. Cited in 2 publications. Immunogen corresponding to Synthetic Peptide within Human GLRX3 aa 250 to C-terminus.

View Alternative Names

PICOT, TXNL2, HUSSY-22, GLRX3, Glutaredoxin-3, PKC-interacting cousin of thioredoxin, PKC-theta-interacting protein, Thioredoxin-like protein 2, PKCq-interacting protein

2 Images

Supplier Data

Immunoprecipitation - Anti-PICOT antibody (AB226396)

PICOT was immunoprecipitated from HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate (prepared using NETN lysis buffer; 1 mg for IP, 20% of IP loaded) with ab226396 at 6 μg/mg lysate. Western blot was performed from the immunoprecipitate using ab226396 at 0.4 μg/ml.

Lane 1 : ab226396 IP in HEK-293T whole cell lysate.

Lane 2 : Control IgG IP in HEK-293T whole cell lysate.

Detection : Chemiluminescence with exposure time of 10 seconds.

All lanes:

Immunoprecipitation - Anti-PICOT antibody (ab226396)

Predicted band size: 37 kDa

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Supplier Data

Western blot - Anti-PICOT antibody (AB226396)

Lysates prepared using NETN lysis buffer.

All lanes:

Western blot - Anti-PICOT antibody (ab226396) at 0.1 µg/mL

Lane 1:

HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 50 µg

Lane 2:

HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate at 50 µg

Lane 3:

Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate at 50 µg

Lane 4:

TCMK-1 (mouse kidney epithelial cell line) whole cell lysate at 50 µg

Lane 5:

NIH/3T3 (mouse embryo fibroblast cell line) whole cell lysate at 50 µg

Predicted band size: 37 kDa

true

Exposure time: 10s

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Mouse, Human

Applications

IP, WB

applications

Immunogen

Synthetic Peptide within Human GLRX3 aa 250 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

O76003

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "2-10 µg/mg of lysate", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/2000 - 1/10000", "WB-species-notes": "" }, "Mouse": { "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/2000 - 1/10000", "WB-species-notes": "" }, "Rat": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Chimpanzee": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Cow": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Dog": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Orangutan": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Pig": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rabbit": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rhesus monkey": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Sheep": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Purification notes

ab226396 was affinity purified using an epitope specific to PICOT immobilized on solid support.

Storage buffer

pH: 7 - 8 Preservative: 0.09% Sodium azide Constituents: Tris citrate/phosphate

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

PICOT also known as thioredoxin-interacting protein (TXNIP) or Vitamin D3 up-regulated protein 1 (VDUP1) is a protein with a mass of approximately 39 kDa. It interacts with several cellular components including thioredoxin acting as a regulatory inhibitor. PICOT is expressed in a variety of tissues with notable presence in heart and muscle tissues. It influences the redox state of the cell playing a significant role in oxidative stress response.

Biological function summary

PICOT acts as a regulator of the thiol redox system in cells. It forms a complex with thioredoxin thereby inhibiting its activity which leads to an accumulation of oxidized proteins. This activity makes PICOT an important player in redox signaling affecting cellular responses to oxidative stress. Through its interactions PICOT contributes to cellular processes such as apoptosis differentiation and proliferation mediated by redox changes.

Pathways

PICOT is integral to the cellular oxidative stress response and redox regulation pathways. It interacts with proteins like thioredoxin and possibly influences NADPH oxidase activity. By modulating thioredoxin PICOT impacts the NF-kB signaling pathway which plays a role in inflammation and immune responses. These pathways highlight PICOT's involvement in maintaining cellular homeostasis especially in stress conditions.

PICOT has links to diabetes and cardiovascular disorders. It affects glucose metabolism and insulin signaling connecting to proteins such as insulin receptor substrates. PICOT also involves in heart disorders given its role in cardiac muscle stress responses and connection to proteins like troponin. Understanding these relationships can aid in developing therapeutic approaches for managing oxidative stress-related diseases.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Together with BOLA2, acts as a cytosolic iron-sulfur (Fe-S) cluster assembly factor that facilitates [2Fe-2S] cluster insertion into a subset of cytosolic proteins (PubMed : 26613676, PubMed : 27519415). Acts as a critical negative regulator of cardiac hypertrophy and a positive inotropic regulator (By similarity). Required for hemoglobin maturation (PubMed : 23615448). Does not possess any thyoredoxin activity since it lacks the conserved motif that is essential for catalytic activity.

See full target information GLRX3

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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Cells 12: PubMed37887353

2023

SLC38A5 Modulates Ferroptosis to Overcome Gemcitabine Resistance in Pancreatic Cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Myeong Jin Kim,Hyung Sun Kim,Hyeon Woong Kang,Da Eun Lee,Woosol Chris Hong,Ju Hyun Kim,Minsoo Kim,Jae-Ho Cheong,Hyo Jung Kim,Joon Seong Park

Scientific reports 11:21520 PubMed34728688

2021

Genetic variants in histone modification regions are associated with the prognosis of lung adenocarcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Hyo-Gyoung Kang,Yong Hoon Lee,Shin Yup Lee,Jin Eun Choi,Sook Kyung Do,Mi Jeong Hong,Jang Hyuck Lee,Ji Yun Jeong,Young Woo Do,Eung Bae Lee,Kyung Min Shin,Won Kee Lee,Sun Ha Choi,Hye Won Seo,Seung Soo Yoo,Jaehee Lee,Seung Ick Cha,Chang Ho Kim,Sukki Cho,Sanghoon Jheon,Jae Yong Park

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-PICOT antibody

Immunoprecipitation - Anti-PICOT antibody (AB226396)

Western blot - Anti-PICOT antibody (AB226396)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (2)

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