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AB251842

Anti-PID1 antibody

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Rabbit Polyclonal PID1 antibody. Suitable for IHC-P, ICC/IF and reacts with Human samples. Immunogen corresponding to Recombinant Fragment Protein within Human PID1 aa 150 to C-terminus.

View Alternative Names

NYGGF4, PCLI1, HMFN2073, PID1, P-CLI1, Phosphotyrosine interaction domain-containing protein 1, Protein NYGGF4

2 Images
Immunocytochemistry/ Immunofluorescence - Anti-PID1 antibody (AB251842)
  • ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-PID1 antibody (AB251842)

PFA-fixed, Triton X-100 permeabilized U-251 MG (human brain glioma cell line) cells stained for PID1 (green) using ab251842 at 4 µg/ml in ICC/IF.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PID1 antibody (AB251842)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PID1 antibody (AB251842)

Paraffin-embedded human liver tissue stained for PID1 using ab251842 at 1/200 dilution in immunohistochemical analysis.

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

IHC-P, ICC/IF

applications

Immunogen

Recombinant Fragment Protein within Human PID1 aa 150 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

Q7Z2X4

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/200 - 1/500", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "0.25-2 µg/mL", "ICCIF-species-notes": "<p>Fixation/Permeabilization: PFA/Triton X-100.</p>" }, "Mouse": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7.2 Preservative: 0.02% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine)
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

PID1 also known as Phosphotyrosine Interaction Domain-Containing 1 is a protein with a molecular mass of approximately 124 kDa. This protein serves significant mechanical roles within the cell involved in various signaling pathways. PID1 expresses itself prominently in adipose tissue skeletal muscle and cardiac tissues marking its potential impact on metabolic processes. Researchers sometimes refer to PID1 as NYGGF4 especially in studies relating to energy metabolism and insulin sensitivity.
Biological function summary

This protein plays notable roles in cellular metabolism and energy management. Although not traditionally seen as part of a complex PID1's function influences mitochondrial activity and insulin signaling pathways. It modulates the activation of several metabolic enzymes and receptors impacting glucose uptake and lipid metabolism. Researchers have highlighted its role in regulating mitochondrial biogenesis hinting at broader implications for energy expenditure within cells.

Pathways

PID1 interacts importantly within the insulin receptor signaling and AMP-activated protein kinase (AMPK) pathways. Its involvement in insulin receptor signaling places PID1 at a nexus point with proteins like insulin receptor substrate 1 (IRS-1) and glucose transporter type 4 (GLUT4) contributing directly to glucose homeostasis. Through AMP-activated protein kinase pathways PID1 plays a role in cellular energy status sensing adjusting cellular energy outputs in response to changes in nutrient availability.

PID1 has links to metabolic syndrome and type 2 diabetes. Its influence on insulin signaling pathways implies that altered PID1 function or expression may contribute to insulin resistance a hallmark of type 2 diabetes. Furthermore studies suggest that in metabolic syndrome PID1 potentially interacts with proteins like peroxisome proliferator-activated receptor gamma (PPARγ) offering insights into adipocyte differentiation and lipid handling. This connection makes PID1 a potential target for therapeutic strategies addressing metabolic and insulin-related disorders.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Increases proliferation of preadipocytes without affecting adipocytic differentiation.
See full target information PID1
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions
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Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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