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AB231315

Anti-PIDD1 antibody

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(2 Publications)

Rabbit Polyclonal PIDD1 antibody. Suitable for WB, IHC-P and reacts with Mouse samples. Cited in 2 publications. Immunogen corresponding to Recombinant Fragment Protein within Mouse Pidd1 aa 650 to C-terminus.

View Alternative Names

Lrdd, Pidd, Pidd1, p53-induced death domain-containing protein 1, Leucine-rich repeat and death domain-containing protein

3 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PIDD1 antibody (AB231315)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PIDD1 antibody (AB231315)

Formalin-fixed, paraffin-embedded mouse kidney tissue stained for PIDD1 using ab231315 at 20 μg/ml in immunohistochemical analysis. DAB staining.

Western blot - Anti-PIDD1 antibody (AB231315)
  • WB

Supplier Data

Western blot - Anti-PIDD1 antibody (AB231315)

All lanes:

Western blot - Anti-PIDD1 antibody (ab231315) at 2 µg/mL

All lanes:

Mouse brain lysate

Predicted band size: 83 kDa

false

Western blot - Anti-PIDD1 antibody (AB231315)
  • WB

Supplier Data

Western blot - Anti-PIDD1 antibody (AB231315)

All lanes:

Western blot - Anti-PIDD1 antibody (ab231315) at 2 µg/mL

All lanes:

Recombinant mouse PIDD1 protein

Predicted band size: 26 kDa,83 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Mouse

Applications

WB, IHC-P

applications

Immunogen

Recombinant Fragment Protein within Mouse Pidd1 aa 650 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

Q9ERV7

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.2-2 µg/mL", "WB-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "5-20 µg/mL", "IHCP-species-notes": "<p></p>" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Purification notes
ab231315 was purified by antigen-specific affinity chromatography followed by Protein A affinity chromatography.
Storage buffer
pH: 7.4 Preservative: 0.011% Proclin 300 Constituents: PBS, 55.77% Glycerol (glycerin, glycerine)
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

PIDD1 also known as p53-induced protein with a death domain has a significant role in the cellular response to damage. It is a 101 kDa protein that contains a leucine-rich repeat and a death domain. PIDD1 expresses in a variety of tissues but researchers detect it more in places like the liver and kidney. Its expression often increases in response to stress signals particularly those leading to DNA damage. PIDD1's structure enables it to interact with other proteins to execute its functions.
Biological function summary

PIDD1 plays a role in regulating apoptosis and cell survival. It can exist as part of a multiprotein complex called the PIDDosome where it partners with RAIDD and pro-caspase-2 to mediate apoptosis. In this context PIDD1 can trigger caspase-dependent apoptotic pathways highlighting its influence on cell fate decisions. Additionally it participates in the DNA damage response linking stress signals to apoptotic outcomes an essential function for maintaining cellular integrity.

Pathways

The protein interacts within the p53 signaling and apoptosis pathways. These pathways integrate cellular responses to stress and PIDD1 functions within these processes by facilitating connections between upstream DNA damage sensors and downstream effector proteins like caspase-2. PIDD1's involvement in signaling cascades helps synchronize responses critical for cell cycle regulation and repair operating alongside proteins such as p53 and RAIDD.

PIDD1 is linked to cancer and neurodegenerative diseases. The protein's role in apoptosis and the cell cycle establishes a connection with tumorigenesis particularly where altered apoptosis contributes to cancer development. Mutations or dysregulation of PIDD1 may impact its interaction with p53 a critical player in many cancers. Furthermore in neurodegenerative diseases the dysregulation of apoptotic processes involving PIDD1 could contribute to neuronal loss. These associations highlight the importance of PIDD1 in both promoting cellular homeostasis and contributing to disease pathology.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Component of the DNA damage/stress response pathway that functions downstream of p53/TP53 and can either promote cell survival or apoptosis (PubMed : 10973264). Associated with CRADD and the CASP2 caspase, it forms the PIDDosome a complex that activates CASP2 and triggers apoptosis. Associated with IKBKG and RIPK1, it enhances sumoylation and ubiquitination of IKBKG which is important for activation of the transcription factor NF-kappa-B (By similarity).
See full target information Pidd1
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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

iScience 28:113081 PubMed40740498

2025

Desmosomal cadherin tension loss in pemphigus vulgaris mediated by the inhibition of active RhoA at cell-cell adhesions.

Applications

Unspecified application

Species

Unspecified reactive species

Xiaowei Jin,Jordan Rosenbohm,Eunju Kim,Bahareh Tajvidi Safa,Amir Ostadi Moghaddam,Kristina Seiffert-Sinha,Merced Leiker,Elijah Jones,Haiwei Zhai,Sindora R Baddam,Grayson Minnick,Yucheng Huo,James K Wahl,Fanben Meng,Changjin Huang,Jung Yul Lim,Daniel E Conway,Animesh A Sinha,Ruiguo Yang

Cell metabolism 34:1548-1560.e6 PubMed36041455

2022

PIDDosome-SCAP crosstalk controls high-fructose-diet-dependent transition from simple steatosis to steatohepatitis.

Applications

Unspecified application

Species

Unspecified reactive species

Ju Youn Kim,Lily Q Wang,Valentina C Sladky,Tae Gyu Oh,Junlai Liu,Kaitlyn Trinh,Felix Eichin,Michael Downes,Mojgan Hosseini,Etienne D Jacotot,Ronald M Evans,Andreas Villunger,Michael Karin
View all publications
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Product promise

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