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AB318187

Anti-PIEZO2 antibody [EPR27152-48]

  • 20ul selling size
  • Recombinant
  • RabMAb
  • KO Validated
  • What is this?

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(1 Publication)

Rabbit Recombinant Monoclonal PIEZO2 antibody. Suitable for WB and reacts with Human samples. Cited in 1 publication.

View Alternative Names

C18orf30, C18orf58, FAM38B, Piezo-type mechanosensitive ion channel component 2, Protein FAM38B

2 Images
Western blot - Anti-PIEZO2 antibody [EPR27152-48] (AB318187)
  • WB

Supplier Data

Western blot - Anti-PIEZO2 antibody [EPR27152-48] (AB318187)

Negative control : T-47D, MCF7.

Samples are non-boiled as boiling may cause protein aggregation.

In Western blot, Anti-Vinculin antibody [EPR8185] (ab129002) staining at 1/10000 dilution.

All lanes:

Western blot - Anti-PIEZO2 antibody [EPR27152-48] (ab318187) at 1/1000 dilution

Lane 1:

MKL-1 (human Skin Loosely packed floati aggregates with irregular outline and no central necrosis) whole cell lysate at 48 µg

Lane 2:

T-47D (human ductal breast epithelial tumor epithelial cell) whole cell lysate at 48 µg

Lane 3:

MCF7 (human breast adenocarcinoma epithelial cell) whole cell lysate at 48 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Observed band size: 320 kDa,124 kDa

false

Exposure time: 92s

Western blot - Anti-PIEZO2 antibody [EPR27152-48] (AB318187)
  • WB

Supplier Data

Western blot - Anti-PIEZO2 antibody [EPR27152-48] (AB318187)

Samples are non-boiled as boiling may cause protein aggregation.

In Western blot, Anti-Vinculin antibody [EPR8185] (ab129002) staining at 1/10000 dilution.

All lanes:

Western blot - Anti-PIEZO2 antibody [EPR27152-48] (ab318187) at 1/1000 dilution

Lane 1:

HeLa (human cervical adenocarcinoma epithelial cell) transfected with scrambled siRNA control whole cell lysate at 20 µg

Lane 2:

HeLa transfected with siRNA specifically targeting PIEZO2 whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Observed band size: 320 kDa,124 kDa

false

Exposure time: 92s

  • Carrier free

    Anti-PIEZO2 antibody [EPR27152-48] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR27152-48

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Complementary Products

Primary Antibodies

AB259949

Anti-FAM38A/PIEZO1 antibody [EPR23826-103]

20ul selling size|KO Validated|RabMAb|Recombinant

Western blot - Anti-FAM38A/PIEZO1 antibody [EPR23826-103] (AB259949)

  • 1
  • 1
Secondary Antibodies

AB150077

Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488)

Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (AB150077)

  • 5
  • 20
Primary Antibodies

AB305299

Anti-TRPV1 antibody [EPR25057-67]

BOND RX™ Validated|20ul selling size|RabMAb|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TRPV1 antibody [EPR25057-67] (AB305299)

  • 0
  • 0
Primary Antibodies

AB7817

Anti-alpha smooth muscle Actin antibody [1A4]

KO Validated

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-alpha smooth muscle Actin antibody [1A4] (AB7817)

  • 5
  • 105
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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "IHCFr" : {"fullname" : "Immunohistochemistry (Frozen sections)", "shortname":"IHC-Fr"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "<p></p>", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>", "IHCFr-species-checked": "notRecommended", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>", "IHCFr-species-checked": "notRecommended", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "<p></p>", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>", "IHCFr-species-checked": "notRecommended", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "<p></p>", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }
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Product details

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

PIEZO2 also known as FAM38B is a mechanosensitive ion channel protein. It has a high molecular mass of approximately 287 kDa. This protein exists in mechanosensitive tissues such as the skin bladder and lungs. PIEZO2 detects mechanical stimuli by opening its ion-conducting pore allowing the influx of cations into the cell which sends a signal.
Biological function summary

PIEZO2 plays an important role in the sensory pathways of touch and proprioception. It is not a part of a larger protein complex but operates as a homotrimer. The channel's sensitivity to mechanical forces enables cells to transduce physical touch into electrical signals facilitating an organism's ability to perceive its environment.

Pathways

Sensory transduction and mechanotransduction heavily involve PIEZO2. This protein interacts within pathways that transmit information about mechanical loads to the nervous system. Although not directly part of larger pathways' cascades its role is well observed in touch-related signaling paths where it occasionally interacts with proteins like TRPV1.

Mutations in PIEZO2 relate to Gordon Syndrome and Marden-Walker Syndrome. Dysfunction in PIEZO2 can lead to defects in touch and balance implicating disorders with sensory processing. There is also a notable connection to the protein TRPA1 which participates in similar sensory pathways and can correlate with sensory-related dysfunctions.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Pore-forming subunit of the mechanosensitive non-specific cation Piezo channel required for rapidly adapting mechanically activated (MA) currents and has a key role in sensing touch and tactile pain (PubMed : 37590348). Piezo channels are homotrimeric three-blade propeller-shaped structures that utilize a cap-motion and plug-and-latch mechanism to gate their ion-conducting pathways (PubMed : 37590348). Expressed in sensory neurons, is essential for diverse physiological processes, including respiratory control, systemic metabolism, urinary function, and proprioception (By similarity). Mediates airway stretch sensing, enabling efficient respiration at birth and maintaining normal breathing in adults (By similarity). It regulates brown and beige adipose tissue morphology and function, preventing systemic hypermetabolism (By similarity). In the lower urinary tract, acts as a sensor in both the bladder urothelium and innervating sensory neurons being required for bladder-stretch sensing and urethral micturition reflexes, ensuring proper urinary function (PubMed : 33057202). Additionally, PIEZO2 serves as the principal mechanotransducer in proprioceptors, facilitating proprioception and coordinated body movements (By similarity). In inner ear hair cells, PIEZO1/2 subunits may constitute part of the mechanotransducer (MET) non-selective cation channel complex where they may act as pore-forming ion-conducting component in the complex (By similarity). Required for Merkel-cell mechanotransduction (By similarity). Plays a major role in light-touch mechanosensation (By similarity).
See full target information PIEZO2
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

BMC musculoskeletal disorders 26:238 PubMed40069682

2025

mpECs with high Piezo2 expression promote fracture healing by driving angiogenesis through the Notch signaling pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Lingfeng He,Fanyong Gong,Jiangyong Wang,Yi Huang,Haifeng Wang
View all publications
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Product promise

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